*3.2. Gene Structure, Conserved Motifs, and Cis-Acting Elements Analyses*

The gene structure analysis revealed that all of the sixteen *GhSAMS* genes are intronless and contain only one exon (Figure 2a). In total, we identified five (5) conserved motifs in the sequence of the *GhSAMS* genes. Both *GhSAMS* genes contained the five motifs, except for *Gh\_A07G1193*, which lacked motifs 3 and 5 (Figure 2b). Furthermore, the cis-acting regulatory elements analysis in the promoter region of the *GhSAMS* genes indicated that they might be primarily involved in the plant defense and stress responsiveness within the plant cells, considering the phytohormonal signals (Table S4).

**Figure 2.** *GhSAMS* genes' structure (**a**), and conserved motifs in their promoter region (**b**).
