*2.1. Wheat TUBBY Family Genes Identification*

We retrieved the protein sequences of TUBBY genes from the Arabidopsis [17] and wheat [18] using the Hidden Markov Model (HMM). The extracted *Triticum aestivum L.* protein sequences were analyzed using CD-search NCBI (http://www.ncbi.nlm.nih.gov/ Structure/cdd/wrpsb.cgi, http://www.ebi.ac.uk/interpro/search/sequence/) and SMART (http://smart.embl-heidelberg.de/) (accessed on 7 March 2022) databases. Proteins that do not exhibit the TUBBY domain were excluded. The chemical properties of TaTUBBY proteins were examined using the Expasy online server (http://web.expasy.org/protparam/) (accessed on 7 March 2022). The CELLO2GO [19] online server was used to predict the subcellular location of TaTUBBY genes.
