*Article* **Genome-Wide Identification, Characterization, and Expression Analysis of TUBBY Gene Family in Wheat (***Triticum aestivum* **L.) under Biotic and Abiotic Stresses**

**Adil Altaf 1,†, Ahmad Zada 2,†, Shahid Hussain 3, Sadia Gull 4, Yonggang Ding 1, Rongrong Tao 1, Min Zhu 1,5,\* and Xinkai Zhu 1,5,6,\***


**Abstract:** The TUBBY gene family is a group of transcription factors found in animals and plants with many functions. *TLP* genes have a significant role in response to different abiotic stresses. However, there is limited knowledge regarding the TUBBY gene family in *T. aestivum*. Here we identified 40 *TaTLP* genes in wheat to reveal their potential function. This study found that TUBBY (*TaTLP*) genes are highly conserved in wheat. The GO analysis of *TaTLP* genes revealed their role in growth and stress responses. Promoter analysis revealed that most *TaTLPs* participate in hormone and abiotic stress responses. The heatmap analysis also showed that *TaTLP* genes showed expression under various hormonal and abiotic stress conditions. Several genes were upregulated under different hormonal and temperature stresses. The qRT-PCR analysis confirmed our hypotheses. The results clearly indicate that various *TaTLP* genes showed high expression under temperature stress conditions. Furthermore, the results showed that *TaTLP* genes are expressed in multiple tissues with different expression patterns. For the first time in wheat, we present a comprehensive *TaTLP* analysis. These findings provide valuable clues for future research about the role of *TLPs* in the abiotic stress process in plants. Overall, the research outcomes can serve as a model for improving wheat quality through genetic engineering.

**Keywords:** wheat; temperature stress; hormonal stress; genes; *TaTLPs*
