*3.11. Expression Analysis of TaTLP Genes in Different Wheat Tissues*

To further study the responses of *TaTLP* genes against biotic and abiotic stresses, we used qRT-PCR to analyze the expression patterns in various wheat tissues (Figure 10). The results showed that *TaTLP*s were expressed in different tissues. The *TaTLP1* transcript level showed high expression in the leaf compared to other tissues. *TaTLP6* and *TaTLP16* showed significantly high expression levels in the root, leaf, and spikelet. *TaTLP17* and *TaTLP6* showed significantly high expression in the stem and leaf, respectively.

Similarly, *TaTLP3* and *TaTLP4* showed significantly high expression levels in leaf, stem, and spikelet tissues. *TaTLP5* was highly expressed in the leaf and stem compared to other tissues, whereas *TaTLP8* expression level was higher in the leaf. *TaTLP10* was expressed in almost all tissues, and the *TaTLP12* expression level was much higher in spikelets than in other tissues. *TaTLP15* and *TaTLP32* were expressed in all tissues, and the expression level was significantly high in the stem and leaf, respectively. *TaTLP34* showed significantly high expression levels in spikelets. *TaTLP36* showed a high expression level in the root, stem, and spikelet. The various *TaTLPs* were expressed in different tissues at varying levels, indicating that they may play a significant role in wheat against various biotic and abiotic stresses.

**Figure 10.** Expression analysis of *TaTLP* genes in different tissues of wheat.
