*4.7. Cluster Heat Map Analysis*

Further, cluster heat map approach to hierarchical cluster analysis using the average linkage via Ward's method of agglomeration. According to the heatmap, seven jute varieties were divided into three main groups based on their varieties' potential (Figure 6) consistent with PCA. The distribution pattern showed that cluster-I contained Y49 and M33 had the highest mean STI (1.26) values based on physiological and biochemical parameters. Therefore, the varieties in Cluster-I can be considered as low temperature-tolerant potential varieties. On the other hand, three varieties viz. Y1, T8, and Y38 were clustered in Cluster-II represented that sensitive group with the lowest mean STI (0.93). The rest of two varieties (W57 and M18) were found in Cluster-III with moderate mean STI (0.98) value. Comparing

the score plots to the cluster heat map revealed that the PCA score plots corresponded to the HCA scores, suggesting tolerant, intermediate, and sensitive varieties.

**Figure 6.** Cluster heat map results were obtained based on biochemical parameters in lowtemperature stress conditions.
