*3.6. Proteins Structure and Sub-Cellular Localization of Maize HSFs*

The protein structures of maize HSFs were predicted through Alphafold. The predicted models were downloaded to view their 3D structure (Figure 8). The highly conserved DBD is represented by α-helices and β-sheets. The OD can be seen to be linked with DBD through linker residues. Most maize HSFs were predicted to be localized inside the nucleus (Table 5). Exceptions were *Zm*HSF-10 and *Zm*HSF-23 (HSFA7a and HSFA7b). This indicates that class 7 HSFs might possess distinct properties.

**Figure 8.** Protein structure of maize HSFs. The prediction confidence level is presented at the bottom. For detailed information about proteins, see Table 5.
