*Article* **Estimation of Genetic Variances and Stability Components of Yield-Related Traits of Green Super Rice at Multi-Environmental Conditions in Pakistan**

**Imdad Ullah Zaid †, Nageen Zahra †, Madiha Habib, Muhammad Kashif Naeem, Umair Asghar, Muhammad Uzair, Anila Latif, Anum Rehman, Ghulam Muhammad Ali and Muhammad Ramzan Khan \***

> National Institute for Genomics and Advanced Biotechnology (NIGAB), National Agricultural Research Centre, Islamabad 45500, Pakistan; imdadcas@gmail.com (I.U.Z.); nageenzahra@hotmail.com (N.Z.); madihahabib217@gmail.com (M.H.); kashifuaar@gmail.com (M.K.N.); umairasghar308@gmail.com (U.A.); uzairbreeder@gmail.com (M.U.); anilalatif87@gmail.com (A.L.); anumrehman92@hotmail.com (A.R.); chairman@parc.gov.pk (G.M.A.)

**\*** Correspondence: mrkhan@parc.gov.pk; Tel.: +92-5190733808

† These authors contributed equally to this work.

**Abstract:** Identifying adopted Green Super Rice (GSR) under different agro-ecological locations in Pakistan is crucial to sustaining the high productivity of rice. For this purpose, the multi-location trials of GSR were conducted to evaluate the magnitude of genetic variability, heritability, and stability in eight different locations in Pakistan. The experimental trial was laid out in a randomized complete block (RCB) design with three replications at each location. The combined analysis of variance (ANOVA) manifested significant variations for tested genotypes (g), locations (L), years (Y), genotype × year (GY), and genotype × location (GL) interactions revealing the influence of environmental factors (L and Y) on yield traits. High broad-sense heritability estimates were observed for all the studied traits representing low environmental influence over the expression of traits. Noticeably, GSR 48 showed maximum stability than all other lines in the univariate model across the two years for grain yield and related traits data. Multivariate stability analysis characterized GSR 305 and GSR 252 as the highest yielding with optimum stability across the eight tested locations. Overall, Narowal, Muzaffargarh, and Swat were the most stable locations for GSR cultivation in Pakistan. In conclusion, this study revealed that G×E interactions were an important source of rice yield variation, and its AMMI and biplots analysis are efficient tools for visualizing the response of genotypes to different locations.

**Keywords:** variability; heritability; univariate stability analysis; AMMI; GGE biplot analysis
