*Article* **An Evaluation of the Antioxidant Activity of a Methanolic Extract of** *Cucumis melo* **L. Fruit (F1 Hybrid)**

**R. S. Rajasree 1,\*, Sibi P. Ittiyavirah 2, Punnoth Poonkuzhi Naseef 3,\*, Mohamed Saheer Kuruniyan 4, G. S. Anisree <sup>5</sup> and Muhammed Elayadeth-Meethal <sup>6</sup>**


**Abstract:** *Cucumis melo* L. (*C. melo*) is a fruit with many medicinal properties and is consumed in various countries. It is utilised for chronic eczema and to treat minor burns and scrapes. The present study was conducted to evaluate the antioxidant activity of a methanolic extract of *Cucumis melo Linn* (MECM). A coarse powder prepared from the fruit and seeds was extracted with methanol (absolute) by a hot continuous percolation process in accordance with the standard protocols. All the extracts were estimated for potential antioxidant activities with tests such as an estimation of total antioxidant activity, hydroxyl radical and nitric oxide scavenging activity and reducing power ability. The qualitative analysis of the methanolic extract of *C. melo* fruit showed the presence of various phytochemical constituents such as carbohydrates, alkaloids, sterols, phenolic compounds, terpenes and flavonoids. The total antioxidant activity of concentrations of 50, 100 and 200 μg were tested and observed to be 3.3 ± 0.1732, 6.867 ± 0.5457 and 13.63 ± 0.8295 μg of ascorbic acid, respectively. The results also showed significant nitric oxide and DPPH scavenging activities as well as a reducing power activity of MECM. Thus, our results suggest that MECM may serve as a putative source of natural antioxidants for therapeutic and nutraceutical applications.

**Keywords:** antioxidants; methanolic extract; DPPH; *Cucumis melo*
