**1. Introduction**

The resins obtained from members of the genus *Commiphora* are used for the management of microbial infections, wounds, tumors, obesity, pain, inflammation, arthritis, gastrointestinal diseases, and fractures [1]. Plants of *Commiphora gileadensis* (synonymous with *Commiphora opobalsamum*) have many applications in traditional medicine [2]. The bark of the plant is used to treat infected wounds [3], a fact supported by the broad-spectrum antimicrobial activity against both Gram-negative, Gram-positive bacteria, and fungi [4,5]. *C. gileadensis* can disturb the bacterial lectin-dependent adhesion process essential for the survival of *Pseudomonas aeruginosa*. The traditional claims of the ability of balsam obtained from the plant to control infections were also supported by their ability to interfere with bacterial lectin-dependent adhesion [6]. The leaves' methanol extract expressed antiviral activity against two enveloped viruses: HSV-2 and RSV B [7].

**Citation:** Althurwi, H.N.; Salkini, M.A.A.; Soliman, G.A.; Ansari, M.N.; Ibnouf, E.O.; Abdel-Kader, M.S. Wound Healing Potential of *Commiphora gileadensis* Stems Essential Oil and Chloroform Extract. *Separations* **2022**, *9*, 254. https:// doi.org/10.3390/separations9090254

Academic Editor: Ernesto Reverchon

Received: 19 August 2022 Accepted: 6 September 2022 Published: 8 September 2022

**Publisher's Note:** MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.

**Copyright:** © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).

We recently reported on the isolation of four *ent*-verticillane-type diterpenes, namely *ent*-Verticillol, *(13S,14S)-ent*-13,14-epoxyverticillol, *(9S,10S)-ent*-9,10-epoxyverticillol, *(1S,3E, 7E,11R)-*(+)-verticilla-3,7,12(18)-triene, as well as gileadenol with novel diterpene skeleton from the fresh *C. gileadensis* stems' CHCl3 extract. Four triterepenes were also isolated, and the antimicrobial potential of the isolated compounds was demonstrated [8].

The main goal of the current study was the evaluation of the wound healing potential of the different *C. gileadensis* extracts and the essential oil and the correlation of this effect with the antimicrobial activity.

#### **2. Materials and Methods**

#### *2.1. Plant Material*

The plants of *Commiphora gileadensis* (L.) C.Chr were described earlier [8].

#### *2.2. Chemicals*

Hematoxylin, eosin, Masson trichrome, petrolatum, and sorbitan monolaurate were purchased from Merck, KGaA, Darmstadt, Germany. Mueller–Hinton agar, Sabouraud dextrose agar, M\_H broth, S-D broth, M\_H agar, and S-D agar were obtained from Scharlau, Barcelona, Spain. Tween 80 was obtained from Chem Sino, China. All solvents used were of analytical grades.
