*3.2. HPTLC Fingerprint Profile of Aqueous Ethanolic Extracts of P. maderaspatensis* Development of Optimum Mobile Phase

Chromatographic separation studies were carried out on the working standard solution of polyphenols' compounds (1 mg mL−1) in methanol. Initially, various trials were carried out with different solvent systems. Finally, toluene/ethyl acetate/formic acid/methanol (3:3:0.8:0.2) was used for simultaneous determination of ellagic acid, gallic acid, catechin, rutin, kaempferol, and quercetin, showing a sharp and well-defined peak. At room temperature, we obtained well-defined bands upon saturating the chamber with the mobile phase for 30 min. The standard band of ellagic acid, gallic acid, catechin, quercetin, rutin, and kaempferol, along with ethanolic, aqueous ethanolic, and aqueous extracts of *P. maderaspatensis* separated on HPTLC plate, were scanned at 254 nm (Figure 1).
