5.4.4. Statistical Analysis

The expression of the CYP1A1 and GSTP1 genes in the ascending and descending colon was presented as mean (±) SD values for each sample. The results were analyzed with Statistica software (StatSoft Inc., Tulsa, OK, USA). Mean values in the control and experimental groups were compared by a one-way ANOVA with repeated measures based on the dose of ZEN administered to the gilts before puberty. If differences between the groups were found, a post hoc Tukey test was performed to determine which pairs of mean groups were significantly different. In ANOVA, group samples were taken from normally distributed populations with the same variance. If the above assumptions were not met in all cases, the equality of the mean groups was tested using the Kruskal–Wallis rank test and the multiple-comparisons test in ANOVA. Different pairs of groups were identified by multiple post hoc comparisons of the rank means for all groups.

**Supplementary Materials:** The following supporting information can be downloaded at: https:// www.mdpi.com/article/10.3390/toxins14050354/s1. File S1: "Task 4. Validation of chromatographic determination methods for zearalenone, α-zearalenol, and DON in pig feed".

**Author Contributions:** The experiments were conceived and designed by M.M., M.G. and M.T.G. The experiments were performed by M.M., P.B., S.L.-Z., D.L. and M.G. Data were analyzed and interpreted ˙ by M.M., P.B. and M.G. The manuscript was drafted by M.M. and M.G. and critically edited by Ł.Z. and M.T.G. All authors have read and agreed to the published version of the manuscript.

**Funding:** This study was supported by the "Healthy Animal-Safe Food" Scientific Consortium of the Leading National Research Centre (KNOW) pursuant to decision no. 05-1/KNOW2/2015 of the Ministry of Science and Higher Education. The project was financially supported by the Minister of Education and Science under the "Regional Initiative of Excellence" program for 2019–2022, project no. 010/RID/2018/19, amount of funding PLN 12,000,000.

**Institutional Review Board Statement:** All experimental procedures on animals were carried out in accordance with the Polish regulations specifying the conditions for conducting experiments on animals (opinions no. 12/2016 and 45/2016/DLZ issued by the Local Ethics Committee for Animal Experimentation of the University of Warmia and Mazury in Olsztyn, Poland, on 30 November 2016).

**Informed Consent Statement:** Not applicable.

**Data Availability Statement:** The data presented in this study are available in this article and Supplementary Materials.

**Conflicts of Interest:** The authors declare no conflict of interest.

### **References**


Wydawnicza AFM: Kraków, Poland, 2011; pp. 149–161. ISBN 978-83-7571-195-0. Available online: http://hdl.handle.net/11315/ 744 (accessed on 15 November 2011).

