*3.4. Extraction with DES*

The extractions with the DESs and purified water as extraction solvents were performed as follows: 30 mg of powdered PUL extract was mixed with the solvent and stirred at 40 ◦C at 950 rpm for 60 min. Each extract was subsequently centrifuged at 13,000 rpm to separate the supernatant and filtered through a syringe filter prior to analysis. The extract was subjected to HPLC analysis to determine the content of flavonoids.
