2.8.2. Photosynthetic Pigment Determination

To determine the presence of chlorophyll a, chlorophyll b, and carotenoids in fresh pepper newly leaves (one leaf for each replicate), the photosynthetic pigments were tested according to [39,40]. Throughout this technique, photosynthetic pigments were extracted from fresh leaves (0.5 g) using 50 mL of acetone (80%), then the green color was determined spectrophotometrically at 665, 649, and 470 nm after the extract was filtered.
