*2.4. Biocontrol Activity of KOF112 against Gray Mold in Cucumber*

Cucumber leaves were used as the alternative host of *B. cinerea* because it was difficult to evaluate *B. cinerea* symptoms on grapevine leaves. The cucumber-*B. cinerea* pathosystem was used. Large and severe symptoms caused by *B. cinerea* were clearly observed on control or SCD-treated leaves (Figure 6). KOF112 (1 × <sup>10</sup><sup>8</sup> cfu/mL) significantly reduced the disease severity caused by *B. cinerea* compared with control and SCD treatment (Figure 6). *Agrobacterium* sp. isolate CHB3 exhibited no inhibitory effect on gray mold.

**Figure 6.** Suppression of cucumber gray mold by KOF112. Disease severity was evaluated as described in Materials and Methods. Crosses (×) indicate means of two independent experiments with three true leaves. \* *p* < 0.05 compared with control and SCD. The representative symptom of cucumber gray mold on true leaves is shown below the graph. Control, untreated. SCD, treated with 10% SCD. KOF112, treated with 1 <sup>×</sup> <sup>10</sup><sup>8</sup> cfu/mL KOF112. *Agro*, treated with 1 <sup>×</sup> 108 cfu/mL *Agrobacterium* sp. isolate CHB3 used as control isolate with no antifungal activity. Bar, 2 cm.

#### *2.5. Biocontrol Activity of KOF112 against Anthracnose in Strawberry*

Strawberry leaves were used as the alternative host of *C. gloeosporioides* because it was difficult to evaluate *C. gloeosporioides* symptoms on grapevine leaves. The strawberry-*C. gloeosporioides* pathosystem was used. Large symptoms caused by *C. gloeosporioides* were clearly observed on control or SCD-treated leaves (Figure 7). KOF112 (1 × <sup>10</sup><sup>8</sup> cfu/mL) significantly reduced the disease severity caused by *C. gloeosporioides* compared with control and SCD treatment (Figure 7). *Agrobacterium* sp. isolate CHB3 exhibited no inhibitory effect on anthracnose.

**Figure 7.** Suppression of strawberry anthracnose by KOF112. Disease severity was evaluated as described in Materials and Methods. Crosses (×) indicate means of four independent experiments with three leaves. \* *p* < 0.05 compared with control and SCD. Each representative symptom of strawberry anthracnose on detached leaves is shown below the graph. Control, untreated. SCD, treated with 10% SCD. KOF112, treated with 1 <sup>×</sup> <sup>10</sup><sup>8</sup> cfu/mL KOF112. *Agro*, treated with 1 <sup>×</sup> 108 cfu/mL *Agrobacterium* sp. isolate CHB3, used as a control isolate with no antifungal activity. Bar, 1 cm.
