2.8.5. Antioxidant Enzymes Activities Assay

POD and PPO enzyme activity was evaluated in this study to obtain a clear indication of defense-related enzymes. The peroxidase activity (POD) and polyphenol oxidase (PPO) enzymes were assayed according to [38], respectively.

### 2.8.6. Isozyme Electrophoresis

Peroxidase (POD) isozyme electrophoresis was analyzed according to the technique [26], while polyphenol oxidase (PPO) isozyme was recorded using the method [47]. The Gel Doc VILBER LOURMAT approach was used to evaluate and investigate gels. While the gels were saturated, the banding shape was videotaped, and the band's number was declared in each gel lane and computed and correlated with each other. The Helena Densitometer Model Junior 24 was used to determine quantitative band quantity and strength changes.
