*2.1. Isolation and Preliminary Screening*

The isolation from the soil samples allowed obtaining ten pure *Actinomycetes* isolates (Table 1). All isolates were preliminarily evaluated for their antagonistic activity against the two target microorganisms (*Escherichia coli* and *Bacillus megaterium*). The isolates AC1 and RS3 showed the highest biological activity against both tested microorganisms, whereas FG1 showed moderate activity against both tested microorganisms (Table 1). The OL2 isolate showed the highest activity against *E. coli* and the most promising activity against *B. megaterium* (Table 1). Based on the obtained results, the isolates AC1, RS3, and OL2 were selected for molecular identification and further biological assays.
