2.8.3. Estimation of Osmolytes Content

#### Soluble Sugar Determination

The dried shoot's soluble sugar content was estimated using the method [41,42]. The dried shoots (0.5 g) from each treatment were diluted with 5 mL of 30% trichloroacetic acid (TCA) and 2.5 mL of 2% phenol and filtered through filter paper, then 1 mL of the filtrate was treated with 2 mL of anthrone reagent (2 g anthrone/L of 95% H2SO4). 620 nm was used to determine the produced blue-green color.
