*2.3. Determination of MIC and MFC*

To further test the inhibitory activity of "Nitric-extract" on the growth of pathogens, the minimum inhibitory concentration (MIC) and the minimum fungicidal concentration (MFC) were determined, and results are summarized in Table 3. The values of both MIC and MFC for all pathogens were in the range 2–3.5%. In more detail, the highest values of MIC (3.5%) were recorded for *P. expansum* CECT 2278 and *F. saccari* CBS 145949, while the lowest (2%) were recorded for *C. gloeosporioides* C2, *Ph. nicotianae* T3-B-K1A and *Pl. tracheiphilus* Pt 2. Values of MFC were the same as MIC for the majority of the strains. Only for strains *P. commune* CECT 20767, *A. alternata* 646, *Ph. nicotianae* T3-B-K1A and *Ph. citrophthora* Ax1Ar, MFC was higher than MIC, indicating that for these four strains, MIC exerted only a fungistatic effect.

**Table 3.** Minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) determined by the nitric-extract.

