*2.2. Molecular Identification*

The amplification with the primers Y1/Y2 produced amplicons with molecular weight of about 434 bp. No amplicons were observed in the negative control. The amplified DNA were sequenced (BMR Genomics, Padova, Italy), and the obtained sequences were compared with those available in GenBank nucleotide archive using Basic Local Alignment Search Tool software (BLAST) (RKV, MD, USA). The results of sequences analysis of AC1, RS3, and OL2 showed high similarity percentages to the sequences of *Streptomyces* sp., *Streptomyces atratus*, and *Arthrobacter humicola*, respectively, present in GenBank with the following accession numbers: ON241810, ON241816, and ON241806, respectively.


**Table 1.** Antagonistic activity of the *Actinomycetes* isolates.

Note: +++, very high activity; ++, high activity; +, moderate activity; -, no activity. AC1, AC2, and AC3 were isolated from acacia rhizosphere; RS1, RS2, and RS3 were isolated from rosemary rhizosphere; FG1 and FG2 were isolated from strawberry rhizosphere; OL1 and OL2 were isolated from olive rhizosphere. \*, isolates that showed the highest antagonistic effect.
