**5. Conclusions**

It was possible to identify *M. phaseolina* (MW585378 and MW585379) associated with charcoal rot from the crop of peanuts "Virginia Champs" variety, located in the rural communities of Buenavista de Benito Juárez, belonging to the municipality of Chietla in Puebla, Mexico.

The strains *T. koningiopsis* (T-K11) and *T. harzianum* (TH-3) displayed class I of antagonism on the Bell scale. In addition, *T. koningiopsis* (T-K11) displayed the highest rate of development, speed of growth, and percentage of inhibition of PIGR radial growth on *M. phaseolina* (71.11%) under in vitro conditions.

In field conditions, *T. koningiopsis* (T-K11) was more effective in promoting the total growth of the plant reaching a weight of 1417.60 g as well as in reducing the incidence of the disease by 76%.

In the locality of Buenavista de Benito Juárez, belonging to the municipality of Chietla in Puebla, Mexico, *T. koningiopsis* (T-K11) showed higher production (1.60 ± 0.01 t/ha<sup>−</sup>1) as well as the chemical treatment (Cercobin®), which obtained the second highest production, obtaining 64 pods per m2. Lowest production occurred in plants inoculated only with *M. phaseolina* (0.67 ± 0.01 t/ha) where elongated reddish-brown lesions were observed that covered 40% of the total surface of the main root.

**Author Contributions:** Conceptualization, O.R.-A., P.A.-H., and S.J.M.-S.; methodology, A.R.-T., S.J.M.-S., and O.R.-A.; software, C.P.L. and O.R.-A.; validation, P.A.-H., A.R.-T., and O.R.-A.; formal analysis, A.L.-C., C.P.L., and O.R.-A.; resources, O.R.-A. and A.R.-T.; original—draft preparation, P.A.-H., S.J.M.-S., and O.R.-A.; writing—review and editing, P.A.-H. and O.R.-A.; visualization, O.R.- A. and A.L.-C.; supervision, P.A.-H.; project administration, O.R.-A.; funding acquisition, O.R.-A. All authors have read and agreed to the published version of the manuscript.

**Funding:** This research was supported by the program PRODEP 2020 of the Secretaría de educación Pública of Mexico (SEP); the Consejo Nacional de Ciencia y Tecnología (CONACyT) and Benémerita Universidad Autónoma of Puebla.

**Institutional Review Board Statement:** Not applicable.

**Informed Consent Statement:** Not applicable.

**Data Availability Statement:** Informed consent was obtained from all subjects involved in the study.

**Acknowledgments:** The authors are grateful to Consejo Nacional de Ciencia y Tecnología (CONA-CyT) and Instituto de Ciencias at Benémerita Universidad Autónoma of Puebla.

**Conflicts of Interest:** The authors declare no conflict of interest.
