*4.5. Genome Sequencing, Assembly, and Annotation*

DNA extraction from KOF112 was performed using a DNeasy PowerSoil Kit (Qiagen). Genome sequencing, assembly, and annotation were performed as described previously [49]. Briefly, short-read sequencing using a DNBSEQ-G400 sequencer (MGI Tech, Shenzhen, China) and long-read sequencing using the GridION nanopore sequencing platform (Oxford Nanopore Technologies, Oxford, UK) were performed in accordance with the manufacturers' instructions. The high-quality short-read and long-read sequences were assembled using Unicycler (ver. 0.4.7) under default conditions. The annotation was performed by Prokka (ver. 1.13). The draft genome sequence of KOF112 was deposited in DDBJ/ENA/GenBank under accession no. AP024603 (genome) and AP024604 (plasmid).
