4.2.3. Experimental Design and Treatment Plan

Products used in this assay can be found in Table 4. Esquive was applied according to label dosage and manufacturer's instructions and Tessior is a formulation ready for application. LC2017 was applied with the timing and dosage recommended by the manufacturer (Table 4). A total of 15 treatments were set up on grapevines of both cultivars, Touriga Nacional and Cabernet Sauvignon in a completely randomized design, with 10 repetitions per treatment. One shoot was inoculated per grapevine making a total of 150 plants used from each cultivar. The combination of treatments and isolates inoculated can be found in Table 5.

**Table 4.** Treatments tested for control of *Lasiodiplodia theobromae* and *Lasiodiplodia mediterranea* under field conditions.



**Table 5.** Treatment plan designed to assess the efficacy of three products against pruning wound infection by *Lasiodiplodia theobromae* and *Lasiodiplodia mediterranea*. Combinations of products used and inoculations spore solution volume.
