*4.4. Identification of KOF112 by 16S rDNA Sequence Analysis*

Genomic DNA was extracted from the one-day culture of KOF112 in SCD medium using a DNeasy PowerSoil Kit (Qiagen, Hilden, Germany) in accordance with the manufacturer's instructions. PCR conditions for amplifying partial 16S rDNA were as follows: after incubation at 94 ◦C for 5 min, PCR amplification was performed for 30 cycles at 94 ◦C for 30 s, 55 ◦C for 30 s, and 72 ◦C for 1 min, with a final extension step at 72 ◦C for 7 min. The nucleotide sequences of the primer set for amplifying partial 16S rDNA from bacterial genome were as follows: 795F (5 -GGATTAGATACCCTGGTA-3 ) and 1492R (5 -GGYTACCTTGTTACGACTT-3 ). The nucleotide sequences of the amplicons were analyzed using the dye terminator method and subjected to the Basic Local Alignment Search Tool (BLAST, NCBI). Phylogenetic analysis was performed using the partial 16S rDNA of KOF112 and *Bacillus* isolates deposited in the NCBI database. The nucleotide sequences were subjected to the NJ method using Molecular Evolutionary Genetics Analysis software, MEGA10 (www.megasoftware.net, accessed on 7 April 2021).
