4.6.1. Nitric-Extract Dilutions

For the test, "Nitric-extract" was tested in all fruits (orange, lemon and apple) as such (ID02) or as three serial dilutions in sterilized distilled water (sdw) (concentrations; 75%—ID04; 50%—ID06; 25%—ID08). In addition, ID03, ID05, ID07 and ID09 were the respective controls.

In this experiment, four control groups were considered: (i) water (ID01); (ii) a solution of nitric acid (HNO3, 65%) and sodium hydroxide (NaOH, 0.1 g/mL) at the ratio 1:4—they are the solvent and base used for the preparation of "Nitric-extract", respectively, which leads to a water solution of NaNO3 at the concentration 0.002 mol/mL (0.17 g/mL); (iii) NaNO3 0.17 g/mL diluted in sdw at 75%—ID05; (iv) NaNO3 0.17 g/mL diluted in sdw at 50%—ID07; NaNO3 0.17 g/mL diluted in sdw at 25%—ID09 (Table 4).

**Table 4.** List of treatments of the *in vivo* tests with shrimp powder extract (Nitric-extract). Control IDs were obtained by adding sterile distilled water (sdw) to NaNO3 (0.17 g/mL).

