2.6.5. Statistical Analysis

The data obtained from the MTT assay were statistically analyzed using GraphPad Prism 6 software (San Diego, CA, USA), one-way ANOVA, and the Bonferroni test. Control samples were considered as 100% viability for each time point. All the experiments were performed with three biological replicates and each data set is presented as the average of three replicates (mean ± standard deviation). A value of *p* ≤ 0.05 was considered to indicate a statistically significant difference. All experimental controls were represented by MCF-7 cell cultures, where fresh culture media was added instead of nanoparticles treatment and were identically processed as described for each assay separately.
