*3.1. Materials Characterization*

Low-frequency ultrasound exposure, such as cavitation and microwaves, ensured complete destruction and removal of all cellular and bone marrow components, including bone cells, stroma, myeloid cells, and lipids from the spongiosa. Quality control of the scaffold delipidization was performed using physical and biochemical methods listed below. After lyophilization in a sublimation unit, the humidity of the biomaterial did not exceed 5%.

Exposure of the material to hydrochloric acid resulted in the final destruction of bone cells and demineralization of the scaffold.

The shelf life of lyophilized demineralized spongiosa Lyoplast® is three years after gamma-ray sterilization. Storage and transportation of the finished product does not require any special temperature conditions.
