*2.3. Drug Loading in Sericin Nanoparticles*

Doxorubicin was loaded within sericin nanoparticles via the direct method by dissolution into the aqueous phase. The next step involved the addition of the dissolved drug aqueous phase into the acetone organic phase under vigorous stirring at neutral pH of 7.45 (1.25–5 wt.% doxorubicin in NPs). The drug-loaded nanoparticles were also recuperated by water and acetone vaporization. The drug was loaded in all prepared formulations 0.1, 0.25, 0.5, and 1% (*w*/*v*).
