*3.2. Biological Evaluation*

All synthesized furan hybrids were analyzed for their in vitro hydrogen peroxidescavenging activity (HPSA), inhibition albumin denaturation (IAD), metal-chelating activity (MChA), and antitryptic activity (ATA). The results observed in vitro were contrasted with those predicted in silico. The results are presented in Table 1.

**Table 1.** Biological evaluation findings from in vitro and in silico. The IC50 values are expressed for the following activities—hydrogen peroxide-scavenging activity (*HPSA*), inhibition of albumin denaturation (*IAD*), metal-chelating activity (*MChA*), and anti-tryptic activity (*ATA*). As reference points, ascorbic acid (AA), quercetin (Qrc), ibuprofen (Ibu), and ketoprofen (Ket) were used. Lipophilicity (*RM*) is a non-dimensional quantity because it is acquired via thin-layer chromatography and is a function of *Rf*. The terms "calculated anti-inflammatory activity (*cAnti-I*)" and "calculated anti-arthritic activity (*cAnti-A*)" are expressed as Pa (probability "to be active"), and assess the likelihood that the investigated molecule is a member of the sub-class of active compounds (resembles the most typical molecular structures in a subset of "actives" in the PASS training set). The molecule with the highest level of activity has a value of 1. **H1–4**—furan derivatives.

