4.4.4. Broth Microdilution Assay

The standard broth microdilution test was performed on 96-well microtiter plates. The compounds were solubilized in DMSO at a concentration of 10 mg/mL. Serial dilutions of the tested compounds were prepared in sterile broth and the obtained solutions of different concentrations were inoculated with each standardized microbial suspension. Ciprofloxacin and fluconazole (Oxoid) served as positive controls. After incubation for 24 h at 37 ◦C, the MIC values were determined as the lowest concentration of compound required to inhibit the microbial growth, as detected spectrophotometrically (Apollo LB 911 ELISA Reader, Berthold Technologies GmbH & Co. KG, Waltham, MA, USA) [82]. The assays were performed in triplicate.
