*Article* **Evaluation of Antiaging Effect of Sheep Placenta Extract Using SAMP8 Mice**

**Ming-Yu Chou 1, Chi-Pei Ou Yang 2, Wen-Ching Li 3, Yao-Ming Yang 4, Yu-Ju Huang 2, Ming-Fu Wang 3,\* and Wan-Teng Lin 5,\***


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**Abstract:** Widely used in traditional medicine, sheep placenta extract (SPE) is known for its physiological effects such as wound healing, antioxidant, and anti-inflammatory properties. However, the effect of SPE on antiaging is still unclear. In this study, we investigated the effect of SPE on aging through the senescence-accelerated mouse prone 8 (SAMP8) strain. We designed an experiment using both male and female mice randomly divided into 4 groups (n = 10) as follows: Group A—control group; Group B—low-dose SPE (61.5 mg/kg BW/day); Group C—medium-dose SPE (123 mg/kg BW/day); and Group D—high-dose SPE (184.5 mg/kg BW/day). As a result of measuring the aging index parameters such as skin glossiness, spine lordosis, and kyphosis, it was found that the treatment of SPE lowered the aging index. In addition, we found that biochemical parameters such as lactic acid, glucose, ketone bodies, free fatty acids, tumor necrosis factor-alpha (TNF-α), and interleukin 6 (IL-6) were not changed in the experimental group treated with SPE for 13 weeks. Finally, we found that lipid peroxidation (LPO) was decreased, while the activities of catalase and superoxide dismutase (SOD) were significantly increased in the brain tissues of SPE-treated male and female mice. Supplementation of SPE lowered the oxidative stress caused by the aging process in mice without toxicity and decreased the aging index, suggesting the value of SPE as an effective antiaging treatment.

**Keywords:** antiaging; sheep placenta; antioxidant; SAMP8; histology
