*Article* **Synthesis, In Silico, and In Vitro Biological Evaluation of New Furan Hybrid Molecules**

**Stanimir Manolov <sup>1</sup> , Iliyan Ivanov 1,\* , Dimitar Bojilov <sup>1</sup> and Paraskev Nedialkov <sup>2</sup>**


**Abstract:** Herein, we report the synthesis of new hybrid molecules between furan and N-containing heterocyclic compounds such as pyrrolidine, 1,2,3,4-tetrahydroquinoline, 1,2,3,4-tetrahydroisoquinoline, and piperidine. The obtained compounds were fully characterized using 1H- and 13C-NMR, UV-Vis, and HRMS spectra. All compounds were assessed for their anti-inflammatory, anti-arthritic, antioxidant, reducing power ability, and chelating activity. The less lipophilic molecules **H2** (60.1 ± 8.16) and **H4** (62.23 ± 0.83) had almost 12 times higher ATA compared with the used ketoprofen (720.57 ± 19.78) standard. The inhibition of albumin denaturation results makes the newly obtained hybrids potential anti-inflammatory drugs, as the expressed values are higher than the ketoprofen standard (126.58 ± 5.00), except **H3** (150.99 ± 1.16). All four compounds show significant activity regarding the in vitro biological activities, which makes them great candidates for potential future drugs.

**Keywords:** furan; 1,2,3,4-tetrahidroquinoline; 1,2,3,4-tetrahidroisoquinoline; piperidine; pyrrolidine; in silico; in vitro biological activity
