*2.3. Biological Examinations*

#### 2.3.1. VGFER-2 Inhibition

We assessed the in vitro inhibitory potential of compound **7** against VEGFR-2 in comparison to sorafenib, to verify our design, and the several computations conducted that indicated the high affinity of compound **7** for binding to and inhibiting the VEGFR-2 enzyme. Compound **7** showed significant inhibitory activity in vitro (an IC50 value of 25 nM) that was lower than that of sorafenib (35 nM). The in vitro results showed that the compound had a great potential to inhibit VEGFR-2, affirming the in silico studies.
