*2.1. Animals*

We kept animals in transparent plastic cages of 30(W) × 20(D) × 10(H) cm3 in a dust-free automatic control room. We maintained room temperature at 22 ± 2 ◦C, with a relative humidity of 65 ± 5%. We controlled the light cycle using an automatic timer, with daily light and dark periods from 07:00 to 19:00 and 19:00 to 07:00 h, respectively. We provided animals with food and water ad libitum.

#### *2.2. Sheep Placenta Extract Preparation and Dosage*

For our experimental samples, we used dried sheep placenta powder provided by Zhenyuebo International Co., Ltd., produced from the placentas of Ujimqin sheep from Inner Mongolia, manufactured by East Ujimqin Banner Yueyi Biotechnology Co., Ltd., Inner Mongolia, China.

We determined dose conversion using the experimental evaluation method of the Ministry of Health and Welfare. First, we determined the recommended daily human intake per kilogram of body weight. We then expanded the recommended human dose by a factor of 12.3 to obtain the dose for mice in the medium-dose group. We then multiplied this dose by a factor of 0.5 for the low-dose group, and by 1.5 for the high-dose group.
