4.4.2. Agar Diffusion Assay

The antimicrobial activity was screened using an adapted agar diffusion method [81]. Briefly, the bacterial and fungal suspensions were prepared in phosphate-buffered saline from fresh microbial cultures and their density was adjusted to 0.5 McFarland. The compounds were dissolved in DMSO at a concentration of 5 mg/mL and then a volume of 5 μL was spotted on the inoculated plates. The diameters of the growth inhibition zones were measured after incubation for 24 h at 37 ◦C. Standardized discs of ciprofloxacin, 5 μg (Oxoid), and fluconazole, 25 μg (Oxoid) were used as positive controls.
