2.3.2. Metal-Chelating Activity (MChA) on Ferrous Ions

The iron-chelating ability of the furan derivatives was studied by the method in [13]. The ability of the different fractions to chelate Fe2+ was evaluated. The reaction mixture contained 1 mL of sample/standard, 3.7 mL of methanol, and 0.1 mL of 2 mmol/L ferric dichloride. With the addition of 0.2 mL of 5 mmol/L ferrozine, the reaction started. The reaction mixture was vortexed and left for 10 min at room temperature. After reaching equilibrium in the mixture, the absorbance of the sample was measured at 562 nm on a UV-Vis spectrophotometer (Camspec M508, Leeds, UK). The blank was prepared the same way as the test sample, but methanol was added instead of the sample/standard. The results were reported as percent inhibition of ferrozine Fe2+ complex formation with the sample. The mean IC50 value was estimated based on three replicates. Ethylenediaminetetraacetic acid (EDTA)-sodium (Na) (0.5 mg/mL) served as a positive control.
