**5. Conclusions**

In summary, through regression analysis and GWEIS analysis, we observed that the VD was negatively associated with depression and anxiety, and further GWEIS analysis identified multiple candidate genes related to depression and anxiety. The interaction effects observed from the results provide new direction for understanding the genetic research of psychiatric disorders. Further research is needed to clarify the underlying mechanism of gene × VD interaction effects for psychiatric disorders.

**Supplementary Materials:** The following are available online at https://www.mdpi.com/2072-664 3/13/10/3343/s1, Table S1: Present and past depression and/or bipolar affective disorder. Table S2: Generalized anxiety disorder.

**Author Contributions:** Z.Z. had full access to all the data in the study and takes responsibility for the integrity of the data and the accuracy of the data analysis. X.Y. contributed equally to the work as co–senior authors. Y.W., Y.J. and F.Z. conceptualized and designed the study. Z.Z., X.Y., Y.J., Y.W., S.C., P.M., C.L., H.Z., C.P., J.Z., Y.C. and F.Z. contributed in acquisition, analysis, and interpretation of the data. Z.Z. drafted the manuscript. F.Z., X.Y. helped with critical revision of the manuscript for important intellectual content. S.C. performed statistical analysis. Y.W., Y.J. and F.Z. provided administrative, technical, or material support. F.Z. supervised the study. All authors have read and agreed to the published version of the manuscript.

**Funding:** This study was supported by the National Natural Scientific Foundation of China (81922059).

**Institutional Review Board Statement:** This study has been approved by UKB (Application 46478) and obtained participants' health-related records.

**Informed Consent Statement:** Informed consent was obtained from all subjects involved in the study.

**Data Availability Statement:** The UK Biobank data are available through the UK Biobank Access Management System https://www.ukbiobank.ac.uk/ (accessed on 20 December 2020). We will return the derived data fields following UK Biobank policy; in due course, they will be available through the UK Biobank Access Management System.

**Acknowledgments:** This study was conducted using the UK Biobank Resource (Application 46478).

**Conflicts of Interest:** All authors report no biomedical financial interests or potential conflicts of interest.
