*4.6. Compound Isolation*

The extract was applied to MPLC (ODS) using a stepped gradient elution of MeOH-H2O (40:60 to 100:0 for 60 min at 10 mL/min) to yield eight subfractions (Fr.1-Fr.8). Fr.4 was separated by semi-preparative HPLC (YMC-pack ODS, 10 × 250 mm, 3.0 mL/min) to afford **2** (65% MeOH in H2O, 0.1% THF, 8.5 mg, tR = 25 min) and Fr.5 was purified by semi-preparative HPLC to obtain **1** (72% MeOH in H2O, 0.1% THF, 11.0 mg, tR = 24 min). The purity of the compounds was checked by LC-MS and the structures were confirmed by 1H and 13C NMR spectra.

#### *4.7. Biotransformation Assay of* **1** *in A. nidulans*

For the biotransformation assay in *A. nidulans*, compound **1** was dissolved in a minimal amout of DMSO and then added into CD-ST liquid media at a final concentration of 100 μM. The strain *A. nidulans* A1145 was inoculated on the prepared medium and grown for 4 days at 28 ◦C 180 rpm. Meanwhile, a CD-ST medium with equal amount of compound **1** was prepared without any strain, and was cultivated under the same condition as the control. The cultures were then extracted by ethyl acetate and the organic phase was dried, dissolved in methanol and detected by LC-MS.
