*4.3. Sequence Analysis of the SlPKS4 Gene*

The whole genome sequencing data was analyzed by antiSMASH [39]. The phylogenetic analysis was conducted with the MEGA7 software [40], with the amino acid sequences of SlPKS4 and reported PKSs synthesizing isocoumarin derivatives retrieved from National Center for Biotechnology Information (NCBI). The conserved domain of the SlPKS4 protein was scanned by the InterProScan program [41]. Comparative analysis between the gene cluster and other isocoumarin BGCs (Figure S7) was conducted by clinker [42].
