*4.2. Materials and Culture Conditions*

The fungal strain *S. lamellicola* HDN13-430 was isolated from marine sediment collected in Antarctic Pritz Bay. The strain was identified by an internal transcribed spacer (ITS) sequence and the sequence data were submitted to GenBank (GenBank accession number KY794926). The strains were deposited at the Ministry of Education of China, School of Medicine and Pharmacy, Ocean University of China, Qingdao, China.

For RNA isolation, *S. lamellicola* HDN13-430 was cultured at 28 ◦C on media potato dextrose agar (PDA, 20% potato, 2% dextrose, and 2% agar) plates for 5 days, and inoculated into Elenmeyer flasks containing 150 mL of 6 different liquid culture medium for 4 days. For genomic DNA extraction, *S. lamellicola* HDN13-430 was cultured at 28 ◦C on PDA plates for 7 days.

*Escherichia coli* strain XL-1 was used for plasmids preservation and amplification. *Saccharomyces cerevisiae* Y31 was used for in vivo DNA recombination for plasmids construction.

*A. nidulans* A1145 was grown at 28 ◦C in CD (0.1% Glucose, 0.5 *v*/*v*% 20×Nitrate salts, 0.01 *v*/*v*% Trace elements, and 2% agar for solid media) media for sporulation, CDS (0.1% Glucose, 1.2 M D-sorbitol, 0.5 *v*/*v*% 20×Nitrate salts, 0.01 *v*/*v*% Trace elements, and 2% agar for solid media) to screen transformants or in CD-ST (2% starch, 2% Casamino acids, 5 *v*/*v*% 20×Nitrate salts, 0.1 *v*/*v*% Trace elements) media for heterologous expression and compound production. All medias were prepared with appropriate supplements, including 10 mM uridine, 5 mM uracil and/or 0.5 μg/mL pyridoxine HCl and/or 2.5 μg/mL riboflavin, depending on the plasmids being transformed.
