**3. Materials and Methods**

#### *3.1. General Experimental Procedures*

NMR spectra were obtained on JEOLJN M-ECP 600 MHz spectrometers, of which TMS was an internal standard. The optical rotation of new compounds was calculated in MeOH on a JASCOP-1020 digital polarimeter. By using an LTQ Orbitrap XL (Thermo Fisher Scientific, Waltham, MA, USA) mass spectrometer, HRESIMS data were obtained. The spray voltage, capillary voltage, and tube lens were 4.0 kv, 16 v, and 35 v, respectively. The capillary temperature was 275 ◦C with a sheath gas flow rate of 10 arb. unit, and FT full mass spectra were acquired in the positive ionization mode at a resolution of 30,000 with 100–1500 Da mass range. The crude extract of *Pseudogymnoascus* sp. HDN17-933 were analyzed by reversed-phase HPLC (5 × 250 mm YMC C18 column, 5 μm) with a linear gradient of MeOH (A) and 0.1% aqueous TFA (B) from 5% to 100% A over 60 min at a flow rate of 1 mL/min. Column chromatography was carried out using the following chromatographic substrates: silica gel (300–400 mesh; Qingdao Marine Chemical Industrials, Qingdao, China), Sephadex LH-20 (GE Healthcare, Bio-Sinences Corp, Piscataway, NJ, USA). HPLC of the Waters company equipped with a 2998 PDA detector was performed on an ODS column (YMC-Pack ODS-A, 10 × 250 mm, 5 μm, 3 mL/min). UV spectra were carried out on Waters 2487 developed by Waters Corporation, Milford, USA. All Fmoc-amino acids were purchased from GL Biochem Ltd. (Shanghai, China). 2-chlorotrityl chloride resin at 1 mmol scale was purchased from Tianjin Nankai Hecheng S&T Co., Ltd. (Tianjin, China).
