*Article* **Phenotypic Analysis of Fourier-Transform Infrared Milk Spectra in Dairy Goats**

**Bartolo de Jesús Villar-Hernández 1, Nicolò Amalfitano 2, Alessio Cecchinato 2, Michele Pazzola 3, Giuseppe Massimo Vacca <sup>3</sup> and Giovanni Bittante 2,\***


**Abstract:** The infrared spectrum of bovine milk is used to predict many interesting traits, whereas there have been few studies on goat milk in this regard. The objective of this study was to characterize the major sources of variation in the absorbance of the infrared spectrum in caprine milk samples. A total of 657 goats belonging to 6 breeds and reared on 20 farms under traditional and modern dairy systems were milk-sampled once. Fourier-transform infrared (FTIR) spectra were taken (2 replicates per sample, 1314 spectra), and each spectrum contained absorbance values at 1060 different wavenumbers (5000 to 930 <sup>×</sup> cm−1), which were treated as a response variable and analyzed one at a time (i.e., 1060 runs). A mixed model, including the random effects of sample/goat, breed, flock, parity, stage of lactation, and the residual, was used. The pattern and variability of the FTIR spectrum of caprine milk was similar to those of bovine milk. The major sources of variation in the entire spectrum were as follows: sample/goat (33% of the total variance); flock (21%); breed (15%); lactation stage (11%); parity (9%); and the residual unexplained variation (10%). The entire spectrum was segmented into five relatively homogeneous regions. Two of them exhibited very large variations, especially the residual variation. These regions are known to be affected by the absorbance of water, although they also exhibited wide variations in the other sources of variation. The average repeatability of these two regions were 45% and 75%, whereas for the other three regions it was about 99%. The FTIR spectrum of caprine milk could probably be used to predict several traits and to authenticate the origin of goat milk.

**Keywords:** FTIR; mid-infrared; caprine milk; milk absorbance spectra; variance components; sources of variation
