*Article* **Effect of Moisture Content Difference on the Analysis of Quality Attributes of Red Pepper (***Capsicum annuum* **L.) Powder Using a Hyperspectral System**

**Ji-Young Choi 1, Jeong-Seok Cho 1, Kee Jai Park 2, Jeong Hee Choi <sup>1</sup> and Jeong Ho Lim 1,\***


**Abstract:** The variety of characteristics of red pepper makes it difficult to analyze at the production field through hyperspectral imaging. The importance of pretreatment to adjust the moisture content (MC) in the process of predicting the quality attributes of red pepper powder through hyperspectral imaging was investigated. Hyperspectral images of four types of red pepper powder with different pungency levels and MC were acquired in the visible near-infrared (VIS-NIR) and short-wave infrared (SWIR) regions. Principal component analysis revealed that the powders were grouped according to their pungency level, color value, and MC (VIS-NIR, Principal Component 1 = 95%; SWIR, Principal Component 1 = 91%). The loading plot indicated that 580–610, 675–760, 870–975, 1020–1130, and 1430–1520 nm are the key wavelengths affected by the presence of O-H and C-H bonds present in red pigments, capsaicinoids, and water molecules. The R<sup>2</sup> of the partial least squares model for predicting capsaicinoid and free sugar in samples with a data MC difference of 0–2% was 0.9 or higher, and a difference of more than 2% in MC had a negative effect on prediction accuracy. The color value prediction accuracy was barely affected by the difference in MC. It was demonstrated that adjusting the MC is essential for capsaicinoid and free sugar analysis of red pepper.

**Keywords:** red pepper powder; hyperspectral imaging; multivariate analysis; moisture adjustment
