**2. Materials and Methods**

### *2.1. Chemicals and Reagents*

Dimethomorph (DMM, 99.9% purity) was obtained from A Chemtek Inc. (Worcester, MA, USA). The human T-lymphocyte cell line (Jurkat T cells) was obtained from the American Type Culture Collection (ATCC, Manassas, VI, USA); this cell line was derived from the peripheral blood of human T-lymphocyte leukemia cells. Acetone (HPLC grade) was purchased from Merck & Co. (Darmstadt, Germany). RPMI-1640 medium, penicillin/streptomycin, phosphate-buffered saline (PBS), and fetal bovine serum (FBS) were all purchased from HyClone (Logan, UT, USA). The Cell Counting Kit-8 was purchased from Dojindo (Kumamoto, Japan). The Annexin V-FITC/PI detection kit was purchased from Abbkine (Wuhan, China). The Mycoplasma Stain Kit was purchased from Sigma Aldrich (St Louis, Missouri, MO, USA), the TruSeqTM RNA Sample Preparation Kit was purchased from Illumina (San Diego, CA, USA), and the PrimeScript RT Reagent Kit was purchased from Beyotime Biotechnology (Shanghai, China). Unless otherwise specified, the reagents used in this study were of analytical grade.
