*3.3. Effectiveness of Radiant Catalytic Ionization and Ozonation Treatment against Salmonella spp. for Bacterial Suspension of 108 CFU/mL*

The number of reisolated *Salmonella* spp. in the control group ranged from 5.81 log CFU/ egg to 5.89 log CFU/egg, but differences were not statistically significant (*p* > 0.05). (Table 4). In all time variants, the number of reisolated bacteria was higher than at the concentration of 10<sup>5</sup> CFU/mL.

**Table 4.** The final number of *Salmonella* spp. on eggshells contaminated with bacterial suspension of 10<sup>8</sup> CFU/mL with or without the addition of poultry manure.


\*—standard deviation, †—time of action; PM (+)—with poultry manure; PM (−)—without poultry manure CFU–colony forming units; a,b,c, . . . —values marked with different letters differ statistically significant, applies to the entire table.

After 30 min of exposure, the bacterial number ranged from 4.83 log CFU/egg (RCI, 4 ◦C, without poultry manure) to 5.69 log CFU/egg (ozonizer, 20 ◦C, with poultry manure). After RCI-emitter and ozonizer treatment at both tested temperatures, the number of bacteria was significantly different higher with the poultry manure (*p* ≤ 0.05) (Table 4).

After 60 min of exposure, the bacterial number ranged from 3.69 log CFU/egg (RCI, 20 ◦C, without poultry manure) to 5.57 log CFU/egg (ozonizer, 20 ◦C, with poultry manure).

After 120 min of exposure, the lowest number of bacteria was found for RCI at 20 ◦C without poultry manure (2.27 log CFU/egg). The value was significantly different (*p* ≤ 0.05) from the value for the ozonizer under the same experimental conditions. The ozonizer treatment at 20 ◦C with poultry manure caused the lowest reduction (5.40 log CFU/egg). In all time variants, at both temperatures RCI and ozonizer reduced statistically significantly higher the bacterial number in the absence of poultry manure (Table 4).

RCI treatment caused a decline of bacteria ranged from 0.26 log CFU/egg (30 min, 4 ◦C and 20 ◦C, with poultry manure) to 3.54 log CFU/egg (120 min, 20 ◦C, without poultry manure). For ozonizer bacterial reduction ranged from 0.20 log CFU/egg (30 min, 20 ◦C, with poultry manure) to 1.91 log CFU/egg (120 min, 4 ◦C, without poultry manure) (Table 2). RCI treatment statistically significantly higher (*p* ≤ 0.05) reduce of *Salmonella* spp. number in bacterial contamination level 10<sup>8</sup> CFU/mL in 60 min and 120 min-exposure, 20 ◦C, without poultry manure than other tested methods.
