2.7.1. The Coating Antigen and mAb Titers

The optimum coating antigen concentration and mAb dilution were verified. A series of concentrations of PBA-OVA (1, 2, 4, 8 μg/L) were coated on the plates, and the mAb of multiple dilution was successively added. The standard curve was established by the ic-ELISA method.
