*2.5. Assessment of mAb*

The subtype of mAb was determined by an antibody type identification kit. The performance of the mAb were assessed by measuring the IC50 values and cross-reactivity (CR). The CR for 16 PAHs was tested using PYR as the target analyte to estimate the specificity of the mAb.

CR (%) = 100 × IC50 (PYR)/IC50 (other analogues).
