2.7.2. The Working Concentration of HRP-IgG

The effect of the working concentration of HRP-IgG on the performance of ic-ELISA was evaluated. Ninety-six-well microculture plates were coated with the most optimal combination of coating antigen and mAb dilution. The sensitivity of ELISA to various working concentrations of HRP-IgG was tested for assay optimization, which were diluted to 1:4000, 1:5000, 1:6000 and 1:7000 with PBS.
