*2.3. Polymerase Chain Reaction (PCR) Amplification and High-Throughput Sequencing (HTS)*

The ITS1 region of the fungi was amplified with the primer pairs ITS1F (5 -CTTGGTCA TTTAGAGGAAGTAA-3 ) [25] and ITS2R (5 -GCTGCGTTCTTCATCG ATGC-3 ) [26]. PCR was performed on initial denaturation at 95 ◦C for 5 min, 34 cycles of denaturation at 95 ◦C for 45 s, 50 s annealing at 58 ◦C, 60 s elongation at 72 ◦C, and 10 min extension at 72 ◦C. The PCR product was detected by 2% agarose gel electrophoresis and purified using the Universal DNA Purification Kit (DP214) (TIANGEN Biotech Co., Ltd., Beijing, China). Then, the amplicons were sequenced on the IonS5TMXL platform (Thermofisher, Waltham, MA, USA). Raw reads were submitted to the National Center for Biotechnology Information Sequence Read Archive database under the accession numbers SAMN19591296–SAMN19591313.
