*3.2. Effectiveness of Radiant Catalytic Ionization and Ozonation Treatment against Salmonella spp. for Bacterial Suspension of 105 CFU/mL*

The differences in the control group were not statistically significant (*p* > 0.05) (Table 3). The bacterial number reisolated from the eggshell ranged from 2.65 log CFU/egg to 2.75 log CFU/egg. The application of ozonizer and RCI-emitter reduced bacterial number. After 30 min-exposure, the bacterial number ranged from 1.29 log CFU/egg (RCI, 20 ◦C and without poultry manure) to 2.48 log CFU/egg (ozonizer, 20 ◦C and with poultry manure). There were no statistically significantly differences between both methods.

After 60 min-exposure, the bacterial number ranged from 0.07 log CFU/egg (RCI, 20 ◦C and without poultry manure) to 2.36 log CFU/egg (ozonizer, 4 ◦C and with poultry manure). Statistically significant differences (*p* ≤ 0.05) were found between RCI at 20 ◦C without poultry manure and fan under all conditions, and ozonizer at 4 ◦C and 20 ◦C with poultry manure and RCI at 4 ◦C and 20 ◦C with poultry manure.

After 120 min. of exposure, the lowest number of *Salmonella* spp. was noted for RCI at 20 ◦C with or without poultry manure (0.00 log CFU/egg) and for ozonizer at 20 ◦C with and without poultry manure. These values were statistically different (*p* ≤ 0.05) from all methods at 4 ◦C with poultry manure or fan at 20 ◦C with poultry manure (Table 3).

For treatment with RCI bacterial number ranged from 0.30 log CFU/egg (30 min, 4 ◦C, with poultry manure) to BDL (120 min, 20 ◦C, without and with poultry manure). For ozonizer bacterial reduction ranged from 0.26 log CFU/egg (30 min, 20 ◦C, with poultry manure) to 2.73 log CFU/egg (120 min, 20 ◦C, with poultry manure) (Table 2).


**Table 3.** The final number of *Salmonella* spp. on eggshells contaminated with bacterial suspension of 10<sup>5</sup> CFU/mL with or without the addition of poultry manure.

\*—standard deviation, †—time of action; PM (+)—with poultry manure; PM (−)—without poultry manure CFU–colony forming units; a,b,c,. . . —values marked with different letters differ statistically significant, applies to the entire table.

The bacterial number after fan action ranged from 0.96 log CFU/egg (120 min, 4 ◦C, without poultry manure) to 2.62 log CFU/egg (30 min, 20 ◦C, with poultry manure). Moreover, reduction number of bacteria for the fan were the lowest and ranged from 0.13 log CFU/egg (30 min, 4 ◦C and 20 ◦C, with poultry manure) to 1.70 log CFU/egg (120 min, 4 ◦C, without poultry manure).
