*3.1. Effectiveness of Radiant Catalytic Ionization and Ozonation Treatment against Salmonella spp. for Bacterial Suspension of 103 CFU/mL*

The bacterial number reisolated from the eggshell in the control group (not exposed to any technology) ranged from 1.59 log CFU/egg to 1.67 log CFU/egg, and was not significantly different (*p* > 0.05) (Table 1). After 30 min of exposure, the bacterial number ranged from 0.03 log CFU/egg (RCI, 20 ◦C, without poultry manure) to 0.69 log CFU/egg (ozonizer, 20 ◦C, with poultry manure). Statistically significant differences were observed in all bacterial contamination level (4 ◦C with poultry manure, 20 ◦C without poultry manure, 20 ◦C with poultry manure) (Table 1). The 60-min and 120-min application of ozonizer and RCI-emitter decreased the bacteria number to detection limit, which is <100 CFU/egg.

**Table 1.** The final number of *Salmonella* spp. on eggshells contaminated with bacterial suspension of 10<sup>3</sup> CFU/mL with or without the addition of poultry manure.


\*—standard deviation, †—time of action; PM (+)—with poultry manure; PM (−)—without poultry manure CFU–colony forming units; a,b,c,. . . —values marked with different letters differ statistically significant, applies to the entire table.

Treatment with RCI reduced bacteria number ranged from 1.39 log CFU/egg (30 min, 4 ◦C, with poultry manure) to level decrease in bacteria number below the detection limit (BDL) (60 min and 120 min, 4 ◦C and 20 ◦C, without and with poultry manure). For ozonizer reduction in the number of bacteria ranged from 0.98 log CFU/egg (30 min, 20 ◦C, with poultry manure) to BDL (60 min and 120 min, 4 ◦C and with poultry manure, 20 ◦C and without poultry manure, 20 ◦C and with poultry manure) (Table 2).

**Table 2.** Decrease in the number of bacteria [log CFU/egg] on eggshells after treatment.



#### **Table 2.** *Cont.*

\*—decrease in bacteria number below the detection limit, †—time of action; PM (+)—with poultry manure; PM (−)—without poultry manure CFU–colony forming units; a,b,c,. . . —values marked with different letters differ statistically significant, applies to the entire table.

The bacterial number after 60 min and 120 min of fan action was 0.00 log CFU/egg. However, a shorter time of exposure (30 min) reduced the number of bacteria from 0.57 log CFU/egg (20 ◦C and without poultry manure) to 0.98 log CFU/egg (20 ◦C and with poultry manure). The reduction was significantly different (*p* < 0.05) lower compared to RCI-exposure for the same time in all variants.
