*3.4. RNA Sequencing Data Assessment*

In this study, a total of 77.76 Gb of high-quality clean reads were obtained after the unqualified reads were filtered out. The clean reads of each sample in each group reached more than 7.63 Gb, and the sequencing error rate was less than 0.025%. The Q30 base accounted for more than 94.18% and the GC content ranged from 49.4% to 50.36% (Table 2). The statistics indicate that the quality of the sequencing is high enough for further analysis. As shown in Figure 3B, the correlation coefficients between samples (Ctr group, L4 group, and M21 group) were higher than 99%, and the phylogenetic tree analysis results demonstrated that the Ctr group was different from the L4 group and the M21 group, but there was a high correlation between the control group and the L4 group. These results are consistent with expectations, so the results revealed good reliability among the samples [40].

**Table 2.** Statistics and quality estimation of RNA-seq reads.


**Figure 2.** DMM-induced apoptosis in Jurkat T cells. (**A**): Control, (**B**): EC10, (**C**): EC25, (**D**): EC50, (**E**): statistics of the apoptosis data of three independent experiments. Q1, Q2, Q3, and Q4 of the flow cytometry graph indicate dead cells, late apoptotic cells, early apoptotic cells, and normal cells, respectively.
