*2.4. Cell Apoptosis Analysis*

Cell apoptosis was assessed by using an Annexin V-FITC/PI detection kit. Activated Jurkat T cells were diluted to 2 × 105 cells/mL using fresh medium, pipetted into 4 mL of the cell dilutions, seeded in a 6-well multiplate, and treated for 36 h with DMM at final concentrations of EC50, EC25, EC10, and the control group. The cells were collected and washed to remove the medium, resuspended in binding buffer, and incubated with Annexin V-FITC solution and PI solution at normal temperature for 15 min. Apoptotic cells were analyzed by a MoFlo AstriosEQ flow cytometer (Beckman Coulter, Inc., Brea, CA, USA) [28].
