*2.2. Jurkat Cell Culture*

As DMM has low solubility in water, an 11,000 mg/L stock solution of dimethomorph was prepared in acetone without FBS and maintained at −20 ◦C [24]. Final concentrations of DMM in the assay were achieved by their dilution in the culture medium. The final acetone concentration in the medium was less than 0.1% (*v*/*v*). The Jurkat cells were inoculated in RPMI-1640 medium containing 10% (*v*/*v*) heat-inactivated FBS, 100 U/mL of penicillin sodium, and 100 μg/mL of streptomycin solution, and incubated in a humidified atmosphere containing 5% CO2 at 37 ◦C. The cells were kept at the logarithmic phase by passages at 2–3 d intervals. The absence of mycoplasma was routinely checked using the Mycoplasma Stain Kit [25].
