*2.4. Chromatographic Analysis*

2.4.1. Gas Chromatography-Tandem Mass Spectrometry (GC-MS/MS) Analysis

The pesticides (chlorfenapyr, diniconazole, and indoxacarb) were analyzed using an Agilent 7000D GC/TQ with a 7890 B gas chromatograph and a 7693A autosampler (Agilent Technologies, Santa Clara, CA, USA). Chromatographic separation was achieved on a DB-5MS UI column (0.25 mm I.D. × 30 m, 0.25 μm) (Agilent Technologies, Santa Clara, CA, USA). The oven temperature program was as follows: 200 ◦C hold for 0.1 min, increased to 250 ◦C at a rate of 40 ◦C/min, and increased to 300 ◦C at a rate of 60 ◦C/min and hold for 5 min. One microliter of the sample was injected in splitless mode. The total running time was 7.18 min. Helium gas was used as the carrier gas at a constant flow rate of 1.5 mL/min. Triple quadrupole MS was applied in dynamic multiple reaction monitoring mode with electron ionization at 70 eV. The GC-MS/MS parameters are summarized in Table 2. The ion source and transfer line temperatures were set at 250 and 280 ◦C, respectively. The MassHunter quantitative analysis software (version 10.1) (Agilent Technologies, Santa Clara, CA, USA) was used for data processing.


**Table 2.** Experimental parameters of ten pesticides by GC-MSMS and LC-MSMS.
