*2.4. Generation of Hybridomas and Production of mAb*

Hybridoma cells with highly sensitive ELISA positivity were cloned four times and expanded to screen the monoclonal cell line. Seven days later, the hybridoma cells (106) stably secreting PYR and BaP antibodies were intraperitoneally injected into mice which had been cleared by 0.5 mL FIA. The mice were executed humanly 7–10 days later, and the ascites was gathered and stored at −80 ◦C.
