*3.6. Analysis of Actual Honey Samples*

Thirty actual honey samples were collected from south China, where hookworm is widely distributed. Thirty honey samples were analyzed to assess the feasibility of the method. The results showed that there was no gelsemine, koumine or humantenmine residue in any of the 30 honey samples, which may be because our sampling time was from January to May, so we had not yet reached the full-bloom stage of the Gelsemium flower, or because the alkaloid content in the honey was very low and did not reach the detection limit of the method. In conclusion, according to our test results, there is a high probability that the honey on the market will not cause poisoning, but the overall evaluation of its safety needs more sample sizes and more test data of samples at different times.
