*3.7. Methods to Compare*

Due to the high sensitivity and precision of LC-MS/MS, the current detection methods for toxic substances in honey mostly rely on LC-MS/MS but the laboratory requirements for LC-MS/MS are relatively high, so it is difficult to use and expensive to maintain. However, the long detection time and low sensitivity of liquid chromatography are not enough to meet the needs of detection. Two-dimensional liquid chromatography has the advantages of a short detection time, simple operating system, large injection volume and high sensitivity. Therefore, we compared the previously established LC-MS/MS and 2D-LC methods for

simultaneous quantification of gelsemine, koumine and humantenmine in pig plasma and tissue. Some methods for detecting toxic alkaloids in honey include LC-MS/MS, HPLC and UHPLC-MS [30,35–37]. The 2D-LC method established by us for the simultaneous detection of gelsemine, koumine and humantenmine in honey has a slightly higher limit of quantification than LC-MS/MS, but it is superior to the two methods in accuracy, precision and stability.
