2.8.3. Total Phenolic Content

Phenolic compounds were extracted with methanol (containing 0.1% HCl) as a solvent. One gram of the sample was individually blended with the solvent at a ratio of 1:20 (*w*/*v*) and the extraction was carried out twice at the room temperature. Then the extract was

stored at −18 ◦C until use. The total phenolic content was measured at 750 nm by Optizin UV–Vis spectrophotometer model (Thermo Electron Corporation, Waltham, MA, USA) [50] followed by Folin–Ciocalteu reagent and gallic acid (GA) as the standard. The results were expressed as mg of GA equivalent (GAE)/100 g of FW.
