**4. Conclusions**

The *L. yunnanensis* transcriptome was obtained using an Illumina HiSeq platform. In total, 98,389 unigenes were generated, of which 98,389 unigenes were aligned with the sequences of public databases. Nr, Nt, Pfam, KOG/COG, Swiss-Prot, KEGG, and GO annotated 31,859, 13,853, 22,684, 10,947, 21,416, 9722, and 23,390 unigenes, respectively. EST-SSR specific primer pairs were successfully designed. In addition, MISA was used to identify SSR loci from all unigenes, and a total of 15,384 SSRs was identified. The repeat motif was given priority with mononucleotides, dinucleotides, and trinucleotides. Furthermore, the 81 primer pairs were synthesized randomly, of which 44 pairs showed effective amplification, 17 primers showed stable amplification, and rich polymorphism was observed in 6 populations. This sequence resource and these potential EST-SSR markers can be used for subsequent population genetic diversity analysis and markerassisted breeding, which is of great significance for formulating resource conservation and utilization strategies for *L. yunnanensis*.

**Author Contributions:** Z.L., H.M. and Y.Z. conceived and designed the research; X.L. (Xi Liu), X.L. (Xiongfang Liu) and Y.L. performed the experiments; Y.Z. and Y.L. analyzed the data and wrote the paper; Y.Z., H.M. and S.Q. revised the manuscript; Z.L. supervised the project. All authors have read and agreed to the published version of the manuscript.

**Funding:** This study was supported by the Major Science and Technology Special Program of Yunnan Science and Technology Department (Grant No. 202102AE090052), and the Ten Thousand Talent Program of Yunnan Province (Grant No. YNWRQNBJ-2019-010).

**Data Availability Statement:** Not applicable.

**Conflicts of Interest:** The authors declare no conflict of interest.

#### **References**

