*2.4. Subcellular Localization Analysis*

*Arabidopsis thaliana* leaf protoplasts were extracted using the *Arabidopsis* Protoplast Preparation and Transformation Kit (Coolaber, Beijing, China) for subcellular localization. The CDSs of *PlNCEDs* were cloned into the 16318-hGFP vector and fused in-frame with the hGFP sequence under the control of the CaMV 35S promoter. The 16318-hGFP empty vector was used as a blank control. After 16 h of incubation in darkness, the green fluorescence protein (GFP) fluorescence was captured by an ultra-high-resolution laser scan confocal microscope (Leica TCS SP8 STED, Wetzlar, Germany).
