*3.3. Conserved Motif and Gene Structure Analysis*

In order to determine if the hierarchical clustering in the phylogenetic tree reflected differences in conserved motifs and/or gene structure, the candidate HhMYBs and AtMYBs were analyzed for motif content and their genes for intron/exon organization. The results (Figure S1) showed that motif 1 was highly conserved and present in most MYB members in *H. hamabo* and *Arabidopsis*, while other motifs showed a larger variability. Notably, the profiles of the HhMYB motif contents differed between the branches of the evolutionary tree and were relatively consistent within each branch. Only a few HhMYB proteins located in the same branch contained different motif profiles. Structural analysis revealed that the majority of HhMYB members (147) had a typical structure containing two introns (Figure S1). However, HhMYBs 1, 6–8, and 10–13 had no introns, whereas other members contained 1 to 15 introns, and HhMYBs with the same number of introns were grouped into a similar subclade.
