**3. Materials and Methods**

#### *3.1. Plant Materials*

We picked the experimental materials from Nujiang Lisu Autonomous Prefecture, Yunnan Province. Inflorescences of *L. yunnanensis* were collected, bottled, and brought back to Kunming for subsequent use. The blooming flowers of five individuals collected within LLO (Table 6) were frozen immediately in liquid nitrogen and stored at −80 ◦C for use as transcriptome sequencing materials. The plant samples were mixed, and RNA was extracted using an RNA extraction kit from TransGen (Beijing, China). Transcriptomic sequencing was performed at Beijing Novogene Biological Information Technology Co., Ltd., Beijing, China. Based on the results of previous investigations into the resource status of *L. yunnanensis*, 6 populations were selected as primer polymorphism detection materials in regions with a dense distribution and adequate quantity, and where sampling was convenient and relatively representative. These were GDX, GCG, FMM, FPK, LLO, and LCM (Table 6).
