*2.4. Research Protocol Innovation*

Continuous updates in technology and tools promote further studies of ornamental plants. Liu et al. selected 14 candidate genes from the transcriptome database of *Lindera megaphylla* for additional qRT-PCRs under different conditions to further verify the reliability of selected reference genes above [15]. Cheng et al. developed an efficient virus-induced gene silencing (VIGS) system using the leaf tip needle injection method, which could effectively silence genes in *Lycoris chinensis*, providing a powerful tool for gene function studies [16].

**Author Contributions:** A.S. and Y.C. wrote the manuscript. All authors have read and agreed to the published version of the manuscript.

**Funding:** This research was funded by the National Natural Science Foundation of China (32172609), the Natural Science Fund of Jiangsu Province (BK20190076) and a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institution.

**Conflicts of Interest:** The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as potential conflicts of interest.
