*3.2. Phylogenetic Analysis of HhMYBs*

In order to analyze the phylogenetic relationships of HhMYBs, a phylogenetic tree was constructed from their predicted coding sequences. MYBs of *A. thaliana* (*AtMYB*s) were included for comparison (Figure 1). The candidate HhMYBs and AtMYBs were clustered within 28 subfamilies but showed differing clustering patterns among the subfamilies, suggesting the divergence of the MYB family during the evolution of these species. The XX subfamily had the most HhMYBs (22), whereas the XIV and XXVII subfamilies each contained only one member, and VII, VIII, X, XXII, and XXVI subfamilies contained no HhMYBs. It is worth noting that several MYB subfamilies were found to contain a greater number of HhMYBs relative to AtMYBs. For example, the III subfamily contains 20 HhMYBs but only 5 AtMYBs. Conversely, some subfamilies consisted exclusively of AtMYBs, indicating that these genes may be lost during the evolution of *H. hamabo*.

**Figure 1.** Phylogenetic tree of MYB family members of *H. hamabo* and *A. thaliana* using the NJ method. The prefix At was used before the names of the *Arabidopsis* MYBs. The subfamilies were represented by different colors.
