*5.2. Phylogenetic Analysis of XTH Proteins*

The Clutsalx-v1.83 program was used to align the XTH sequences from *O. fragrans*, *A. thaliana*, and *O. sativa*. MEGA6.0 was then used for phylogenetic analysis via the neighborjoining (NJ) method with 1000 bootstrap replicates. The online software ITOL (https: //itol.embl.de/ (accessed on 17 March 2022)) was used to annotate the phylogenetic tree.

#### *5.3. Motif Identification and Gene Structure Analysis*

MEME Suite 5.3.0 (http://meme-suite.org/tools/meme (accessed on 17 March 2022)) was used to identify the motifs of OfXTH proteins, at a maximum motif number of 10, with a minimum and maximum width of six and 50, respectively. The online software GSDS (http://gsds.gao-lab.org/ (accessed on 17 March 2022)) was used to predict the gene structure of *OfXTHs*.
