*4.3. Sequence Analysis*

The conserved motifs of RsAP2 genes were identified with MEME (http://memesuite.org/, accessed on 29 June 2022) [46]. The information of gene structures describing the exon and intron was extract from the gff file of genome sequence data. Visualization of the RsAP2 gene structures was conducted by Gene Structure Display Server (GSDS, http://gsds.gao-lab.org/, accessed on 29 June 2022) and Toolbox for Biologists (TBtools, version 1.098774) [47,48].

#### *4.4. Chromosomal Localization and Colinearity Analysis*

We used MapChart (version 2.32) to present the localization information for RsAP2 genes on chromosomes [49]. The duplication and expansion of the RsAP2 gene family was analyzed with MCScanX (http://chibba.pgml.uga.edu/mcscan2/MCScanX.zip, accessed on 30 June 2022), and we used CIRCOS (https://circos.ca/, accessed on 5 July 2022) to complete the figure of RsAP2 gene family collinearity [50].
