**5. Conclusions**

Based on the importance of the MYB family in plants and the strong adaptability of *H. hamabo* to stressed environments, genome-wide identification and analysis of the MYB transcription factor family in H. hamabo were performed for the first time in this study. The search for MYB family members in the *H. hamabo* genome led to the identification of 204 putative HhMYB family members. The HhMYB family was clustered into 28 subfamilies with AtMYBs according to phylogenetics and their motif patterns. In addition, we found that the 204 *HhMYB* genes were unevenly distributed across the 46 chromosomes of *H. hamabo*. Expression analysis with transcriptomic data and qRT-PCR showed that the candidate HhMYB family displays a wide range of sensitivities to abiotic stress and hormones. Several *HhMYB*s were involved in the plant responses to both saline and drought stresses to varying degrees, indicating that these *HhMYB*s may have regulatory functions in tolerance to saline and drought stress and deserve further investigation. Our results provide a foundation for further researching MYB gene functions in *H. hamabo.*

**Supplementary Materials:** The following are available online at https://www.mdpi.com/article/ 10.3390/plants12071429/s1. Figure S1: Phylogeny (a), conserved motif (b), and gene structure (c) analyses of HhMYBs; Figure S2: Chromosome distribution of *HhMYB*s; Table S1: Primer sequences for qRT-PCR; Table S2: List of the MYB family genes in *H. hamabo.*

**Author Contributions:** Conceptualization, C.G. and Z.W.; investigation, D.L. and Z.F.; writing original draft preparation, D.L. and Z.W.; writing—review and editing, Z.W. and C.G. All authors have read and agreed to the published version of the manuscript.

**Funding:** This research was funded by Six Talent Peaks Project of Jiangsu Province [NY-042].

**Institutional Review Board Statement:** Not applicable.

**Informed Consent Statement:** Not applicable.

**Data Availability Statement:** No new data were created or analyzed in this study. Data sharing is not applicable to this article.

**Conflicts of Interest:** The authors declare no conflict of interest.

#### **References**


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