*2.6. Analysis of the Promoter Cis-Element of the HSF Gene in M. sativa*

In order to learn more about how the *MsHSF* gene is regulated, this study examined the cis-acting components of the promoter region. We searched the PlantCARE database for potential cis-acting elements in the 2000 bp upstream sequence of the *MsHSF* translation start codon (Figure 8). As a result, most are stress response elements, and all promoters contain at least one TATA-box, CAAT-box, and short\_function element. The next most common element is the ABRE element (contained in all promoters except *MsHSF11*), which is associated with the abscisic acid response. Other elements present in MsHSF promoters include the MBS element (involved in drought, high salt, and low temperature responses), TC-rich repeat sequences (involved in defense and stress responses), AE-box, and TGA erythromycin response elements. These findings suggest that *MsHSF* genes may be involved in multiple transcriptional regulatory mechanisms of plant growth and stress responses.
