*4.3. Gene Structure and Motif Identification*

The conserved amino acid sequences of HSF proteins were analyzed using the online MEME tool (https://meme-suite.org/meme/) (accessed on 5 August 2022) with the parameters of minimum width of ≥ for 6, maximum width of 50, and number of parentheses of 10; all other parameters were set to default values. Then we obtained the intron-exon distribution of the *MsHSF* gene from the *M. sativa* genome using the GFF annotation method, and finally the results were displayed using TBtools software v1.098661 (Chen, C.J.; Guangzhou, China) [60].
