*4.7. Protein Interaction Network Prediction and GO Enrichment Analysis*

HSF protein sequences were uploaded to the STRING database (https://string-db. org/) (accessed on 19 August 2022) for node comparison, and relationships between important proteins were predicted based on *A. thaliana* protein interactions. Finally, Cytoscape (Shannon, P.; California, USA) [61] was used to visualize the generated networks.

The online software EggNOG-Mapper (http://eggnog-mapper.embl.de/) (accessed on 18 August 2022) was used to annotate the GO function of the *MsHSF* gene. The results were collated using the eggNOG-mapper Helper function of TBtools and the text files used for downstream analysis were exported separately to GO Enrichment for enrichment analysis. Finally, use the online charting tool HIPLOT (https://hiplot.com.cn/) (accessed on 19 August 2022) to view and examine the data.
