*2.1. Identification and Physiochemical Characteristics of NnDofs*

Through the search of the lotus genome by HMM with the Dof domain (PF02701), 29 *NnDof* family members were recognized after further validation in the CDD and Pfam databases. According to the distribution of *Dof* genes on chromosomes, *NnDofs* are named *NnDof1* to *NnDof29*. We investigated the physical and chemical properties of the *NnDof* members. Analysis of NnDof proteins showed amino acid lengths ranging from 105 to 508 aa, molecular weights ranging from 12.1 to 55.4 kDa, and isoelectric points ranging from 5.67 (NnDof13) to 9.96 (NnDof29). The analysis results of the instability index showed that the *NnDof5* value was lower than 40, which indicated that the rest of the *NnDofs* were probably unstable proteins. The aliphatic indexes of NnDof proteins were all between 41.81 and 73.51. GRAVY values for all NnDof proteins ranged from −1.161 to −0.341, indicating that all NnDof members are hydrophilic proteins. The subcellular localization prediction findings indicated 17 NnDof proteins in the nucleus and the remainder are extracellular. Detailed results are listed in Table S1.
