*5.1. Identification and Physical Properties Analysis of NnDofs*

The lotus genome data used in this study are available under the Nelumbo Genome Database (NGD) [63]. All protein sequences from the lotus genome were scanned by HM-MER 3.0 using a hidden Markov model (HMM) of the Dof domain (PF02701), downloaded from the Pfam website (http://pfam.xfam.org/, accessed on 3 May 2021), with an E value of 1 × <sup>10</sup>−5. The presence of the conserved domain of Dof in the predicted protein was confirmed by NCBI Conserved Domain Database (CDD), Pfam, and SMART. Subcellular locations were predicted using ProtComp 9.0 from Softberry (http://www.softberry.com/, accessed on 3 May 2021). Protein molecular weight (Mw) and theoretical isoelectric point (pI), instability index, aliphatic index, and grand average of hydropathicity (GRAVY) were calculated by Expasy (https://web.expasy.org/protparam/, accessed on 3 May 2021).
