*3.4. HPV Overall Positive Agreement between Cervical and First-Void Urine Samples*

Using the clinical cut-offs, the PPA between first-void urine sample and cervical samples was 39/43 = 90.7% and the NPA 19/21 = 90.5%. The OPA was 87.5%, and the Cohen's kappa was 0.79 (good agreement).

Without any clinical cut-off, the PPA was 43/47 = 91.5%; the NPA was 13/17 = 76.5%. The OPA was 95.3%, and the Cohen's kappa was 0.680 (good agreement).

Using the package insert cut-offs of 38.3 Ct for HPV 16 and 34.2 Ct for other HR genotypes for the cervical samples and a clinical cut-off of 40 Ct for the urine samples, the PPA of self-collected urine samples was 42/43 = 97.7%; the NPA was 16/21 = 76.2%. The OPA was 90.6% (58/64), and the Cohen's kappa was 0.776 (good agreement). The results are reported in Table 2.
