*2.6. Onclarity*

The BD Onclarity HPV Assay detects 14 HR-HPV genotypes and co-amplifies a betaglobin internal control (IC) that acts as processing control. The primers for the 14 HPV genotypes are designed to target a region of 79–137 bases in the E6/E7 genome, whereas the IC primers amplify a 75 base region in the human beta-globin gene. The assay consists of three PCR assay tubes (G1, G2, and G3) and four optical channels for the detection of the 14 HR-HPV genotypes (16, 18, 31, 45, 51, 52) as single infections and the remaining eight genotypes in three groups (33/58, 56/59/66, 35/39/68) and the IC.
