*2.4. Library Preparation*

Libraries were prepared using the Illumina DNA prep kit (San Diego, CA, USA) following the manufacturer's instructions, using 450 ng of DNA in 35 µL as input. Sequencing was performed using the Illumina MiSeq instrument and the Illumina MiSeq reagent kit v2 500 cycles (2 × 250 bp). Libraries were normalized to 4 nM in combination with 12.5 pM of PhiX (Illumina).
