*2.3. PCR Target-Enrichment for Deep Sequencing of HPV 16*

HPV 16 whole genome material was amplified using 47 overlapping amplicons described in Cullen et al. [18] and optimized by Arroyo et al. [19] Briefly, primer sets were divided into five different reactions to decrease self-dimer and cross-primer dimer formation. PCRs were performed using Qiagen Multiplex PCR Master Mix (Qiagen, Hilden, Germany) and 0.2 µM of each primer, according to manufacturers' instructions. PCR amplification products were pooled together according to sample name prior to library preparation.
