*2.2. Hybrid Capture 2*

Qiagen HC2 test is a sandwich capture molecular hybridization assay: it is a signal amplification detection method based on chemiluminescence that detects 13 HR HPV types: HPV 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, and 68. The DNA:RNA hybrids are captured on a microplate, and the emitted light is measured in a luminometer as relative light units (RLU). Samples were considered as positive if the ratio RLU/cut-off was >1.0 (equivalent to 5000 copies/reaction). All samples with RLU between 1 and 2.5 should be retested, as requested in package insert instructions.
