*2.3. Linear Array*

The Roche Diagnostics Linear Array test uses biotinylated PGMY09/11 consensus primers to amplify a 450-bp region of the L1 gene. It is capable of detecting 37 HPV genotypes: HPV6, 11, 16, 18, 26, 31, 33, 35, 39, 40, 42, 45, 51, 52, 53, 54, 55, 56, 58, 59, 61, 62, 64, 66, 67, 68, 69, 70, 71, 72, 73 (MM9), 81, 82 (MM4), 83 (MM7), 84 (MM8), IS39 e CP6108. The denatured PCR products were then hybridized to an array strip containing immobilized oligonucleotide probes. The results were visually interpreted by using the provided reference guide according to manufacturer's protocol by two independent operators, and the results were compared to reach the final one.
