*Article* **Reference Equation of a New Incremental Step Test to Assess Exercise Capacity in the Portuguese Adult Population**

**Rui Vilarinho 1,2,3,\*, Ana Toledo 2, Carla Silva 2, Fábio Melo 2, Leila Tomaz 2, Luana Martins 2, Tânia Gonçalves 2, Cristina Melo 2, Cátia Caneiras 3,4,5,6 and António Mesquita Montes 2,7**


**Abstract:** Step tests are important in community- and home-based rehabilitation programs to assess patients' exercise capacity. A new incremental step test was developed for this purpose, but its clinical interpretability is currently limited. This study aimed to establish a reference equation for this new incremental step test (IST) for the Portuguese adult population. A cross-sectional study was conducted on people without disabilities. Sociodemographic (age and sex), anthropometric (weight, height, and body mass index), smoking status, and physical activity (using the brief physical activity assessment tool) data were collected. Participants performed two repetitions of the IST and the best test was used to establish the reference equation with a forward stepwise multiple regression. An analysis comparing the results from the reference equation with the actual values was conducted with the Wilcoxon test. A total of 155 adult volunteers were recruited (60.6% female, 47.8 ± 19.7 years), and the reference equation was as follows: steps in IST = 475.52 − (4.68 × age years) + (30.5 × sex), where male = 1 and female = 0, and r<sup>2</sup> = 60%. No significant differences were observed between the values performed and those obtained by the equation (*p* = 0.984). The established equation demonstrated that age and sex were the determinant variables for the variability of the results.

**Keywords:** exercise tests; exercise tolerance; step mode testing; interpretability
