4.1.3. Identification Method for Proteins

MASCOT 2.4.1 (Matrix Science, London, UK) was used to search the Uniprot 2019\_02 (561356 sequences; 201858328 residues) database with bacterial sequences and a filter to examine the spectrum data. These were the search criteria for mascots: With variable carbamidomethyl (C) and oxidation (M) modifications, peptide mass tolerance of 50 ppm and fragment mass tolerance of 0.8 Da. Acceptable protein identification required the identification of at least two peptide fragments per protein.
