*2.3. Histological Validation*

Histological validation of LN was performed by removing the SLN, followed by a systematic pelvic lymphadenectomy. Para-aortic LN were removed if intraoperatively conspicuous or malignant SLN. [99mTc]Tc-Nanocolloide-labeled SLN were localized and identified intraoperatively through a laparoscopic gamma probe (Neoprobe®, Models 1017 and 1100, Devicor Medical Products, Inc., Cincinnati, OH, USA), resected separately, and sent for frozen sectioning. SLN were further ultrastaged with the preparation of the entire LN in 200 μm slices.
