**2. Materials and Methods**

#### *2.1. Subjects*

Five glaucoma patients (see Table 1 for demographic data), besides F.H., with central visual field defects were enrolled in this proof-of-concept study, which followed the tenets of the Helsinki Declaration and was approved by the local university hospital, after giving written informed consent. All subjects already had established glaucoma and met the inclusion criteria for open-angle glaucoma (n = 6, age 50 years or older) with an open anterior chamber and typical glaucomatous optic disc damage defined by a vertical cup ratio ≥ 0.7, retinal nerve fibre layer defect or localized rim depression, and glaucomatous visual field defects [16].


**Table 1.** Demographic data of the subjects.

S: Subject; m: male; f: female; BCVA: Best corrected visual acuity; OD: right eye; OS: left eye; logMAR: logarithm of minimal angle of resolution; MD: mean visual field deviation of 10-2 SITA standard VF; dB: Decibels; \* 10-2 SITA fast protocol.

#### *2.2. Standard Automated Perimetry Check (SAP)*

Visual field defects were assessed using the 10-2 standard algorithm (subjects 1, 3, 4, 5 and F.H.) or 10-2 SITA Fast (subject 2) of the Humphrey Field Analyzer 3 (Carl Zeiss Meditec AG, Jena, Germany). The test stimulus was 4 mm<sup>2</sup> in size (equivalent to a size III Goldmann stimulus, i.e., 0.43◦) and presented for 0.2 s.
