*3.2. CY-09 Inhibited NLRP3 Inflammasome Activation in 3*×*Tg-AD Mice*

Then, Western blot and ELISA were then used to detect the expressions of NLRP3 inflammasome-related proteins to confirm the effect of CY-09 on NLRP3 inflammasome activation. As demonstrated in Figure 1b–h, compared with NTg mice, the expressions of NLRP3, pro-caspase-1, caspase-1(P20), and IL-1β were significantly increased in 3×Tg-AD mice, with the *p*-values lower than 0.05 and 0.01 respectively. Simultaneously, a notable reduction in the expressions of these proteins was found in CY-09 treated 3×Tg-AD mice, with the *p*-values lower than 0.05 and 0.01, respectively. Moreover, the expressions of procaspase-1 and caspase-1 were remarkably decreased in NTg + CY-09 mice, with the *p*-values all lower than 0.01. Except for a significant difference between NTg mice, NTg + CY-09 mice and 3×Tg-AD + CY-09 mice, the results of IL-1β secretion measured by ELISA were consistent with those of Western blotting. No differences were found in the expression of

pro-IL-1β among the four groups of mice. Together, these data suggested that CY-09 has an inhibiting effect on NLRP3 inflammasome activation in the brain of triple transgenic AD mice.
