*3.4. CY-09 Increased Glucose Transport in 3*×*Tg-AD Mice*

GLUTs are responsible for glucose transport, which is the basis of glucose metabolism. GLUT1, GLUT3, and GLUT4 can be expressed in the brain. Here, we used Western blotting to analyze the expression of GLUT1, GLUT3, and GLUT4. As shown in Figure 3a–d, the expression of GLUT1, GLUT3, and GLUT4 were lower in 3×Tg-AD mice than in NTg mice, with the *p*-value lower than 0.01, 0.05, and 0.01 respectively. However, the expressions of GLUT1 and GLUT4 were significantly increased in CY-09 treated 3×Tg-AD mice than in non-treated AD mice, with the *p*-value lower than 0.05. The expression of GLUT3 was increased with a *p*-value of 0.077. Further, immunostaining of GLUT4 demonstrated the decreased distribution in 3×Tg-AD mice and the increased distribution in CY-09 treated 3×Tg-AD mice (Figures 3e and S2). Hence, these data exhibited that inhibiting NLRP3 inflammasome activation can increase the expression and distribution of GLUTs in the 3×Tg-AD mice.
