3.4.5. LPS Biosynthesis

In our experimental evolution, we observed mutations in *lpxc* and *lpxD* at later timepoints of selection in both planktonic and biofilm KP (Figure 4). SNPs in *lpxD* were acquired by days 15–27 for all planktonic populations, while SNPs in *lpxC* were observed on days

27 and 15 for populations 2 and 3, corresponding to a high level of COL-R (512 μg/mL) (Table 3). For biofilm populations 1 and 3, SNPs in *lpxC* were observed by day 15 and 24 at 100% frequency.
