*2.1. Bacterial Strains, Plasmids and Culture Conditions*

Bacterial strains and plasmids used in this work are given in the supplementary materials (Table S1). *E. coli* strains were grown in Luria–Bertani (LB) broth [31] at 37 ◦C, with shaking at 160 rpm and, when necessary, ampicillin at 100 μg/mL (Sigma Aldrich, St Louis, MO, USA) was added to the medium. Strains used as target organisms to assess antibacterial activity were cultivated, without shaking, in brain heart infusion (BHI, Sigma Aldrich, Saint Louis, MO, USA) medium at 37 ◦C for 12–18 h before use.
