*2.2. Melissopalynological Analysis*

The pollen presence is fundamental for the melissopalynological analysis of honey [9]. The presence of pollen in the honey samples and the botanical origins of honey samples was tested according to the recommended protocols [15–17]. Briefly, ten grams of honey was mixed in 20 mL of warm distilled water (40 ◦C), centrifuged for 10 min at 2500 rpm, poured into a small tube, and centrifuged again for 10 min. The entire sediment was put on a slide, spread out over an area of 20 mm2, and dried by slight heating at 40 ◦C. The sediment was mounted with glycerin gelatin and liquefied by heating in a water bath at 40 ◦C [18]. The identification of pollen grain in the treated honey samples was performed according to the pollen atlas [19].
