*2.8. Soluble Protein*

The protein content in honey was determined using the Bradford method according to Latimer [14]. One thousand μL of Bradford reagent (G-250) was added to 20 μL of each honey sample. Samples were incubated for 5 min at room temperature and the absorbance at 595 nm was read using a Biomate 3 spectrophotometer (Thermo Scientific, Waltham, MA, USA). The results were calculated from a calibration curve 0–100 μg/sample (y = 0.0551x, r2 = 0. 9991). Bovine albumin has been used as the standard.
