*3.1. Enzyme-Treated Bee Pollen Alleviates Mice Scratching Behavior*

The itch-associated response is a typical allergic reaction and can be used as an indicator for the evaluation of anaphylaxis levels [32,33]. To reflect the level of anaphylaxis, scratching frequency of mice was recorded. As shown in Figure 1B, the mice in OVA group exhibited the highest scratching frequency after injection. There was no significant difference in the scratching frequency of mice between OVA and BP groups. However, the mice in 2E-BP and 3E-BP groups exhibited significantly less scratching frequency than the mice in OVA and BP groups. This indicated that enzyme-treated bee pollen alleviates mice scratching behavior, and that enzymatic treatment can reduce the allergenicity of bee pollen.
