*3.2. Enzyme-Treated Bee Pollen Mitigates Histopathological Injury in Mice*

Mast cells and granulocytes play important roles in food allergy. Specifically, FcεRI as a kind of IgE receptor existing in mast cells and basophils can be activated by crosslinking with allergen-specific IgE antibodies, leading to the release of allergic mediators responsible for the early- and late-phase of allergic reactions [16,34,35]. Herein, the mast cells and granulocytes in mice spleen were visualized by TB and GIEMSA staining, respectively. As shown in Figure 2, there are significantly more mast cells and granulocytes (marked with red arrows) in OVA and BP groups than in CK group, while there is no significant difference in the 2E-BP and 3E-BP groups compared with the CK group. This revealed that enzyme-treated bee pollen mitigates histopathological injury in mice.

**Figure 2.** Histopathological changes in mouse spleen in different treatment groups. Mast cells and granulocytes stained separately with toluidine blue (TB) and GIMSA are marked with red arrows.
