*2.8. Diastase Activity*

The spectrophotometric method was used to determine the diastase activity (Megazyme International Ireland, Bray Business Park, Bray, Co. Wicklow, Ireland). The sample was dissolved in sodium maleate buffer and its volume was adjusted with water to the volumetric flask's mark. The buffer solution contained Amylazyme tablets (Megazyme International, Ireland). The substrate was hydrolyzed and colored compounds were produced when a A-amylase was present. Following the completion of the reaction, the absorbance of the filtrate was determined at 590 nm. The sample's diastase activity was inversely correlated with the absorbance. Diastase number (DN) was used to calculate diastase activity.
