*2.11. HPTLC Analyses*

The analyses were performed with the use of the CAMAG (Muttenz, Switzerland) HPTLC chromatography set, consisting of a semi-automated sample applicator (Linomat 5), an automatic developing chamber (ADC 2), a derivatizer and a visualizer. The extract samples were applied on the HPTLC plate (HPTLC Silica Gel 60 F254 plates, 20 cm × 10 cm, Merck, Darmstadt, Germany) in a volume of 5 μL, as 8 mm wide bands. Two different systems for the mobile phase and derivatization reagent were used: A–mobile phase: ethyl acetate, acetic acid, formic acid, water (10:1.1:1.1:2.6) and derivatizing agent: Natural Product Reagent/Polyethylene glycol 400 (NP/PEG); B—mobile phase: chloroform, ethyl acetate, formic acid (5:4:1) and derivatizing agent: *p*-anisaldehyde reagent with heating in 110 ◦C (10 min). After derivatization, plates were photographed in visible light and UV (366 nm). The obtained images were analyzed using HPTLC software (Vision CATS, CAMAG, Muttenz, Switzerland).
