2.3.6. Bee Pollen Extracts for the Determination of Phenolic Compounds

The extraction procedure was carried out as follows: 0.1 g of bee pollen was mixed with 25 mL of 80% methanol and ultrasonicated for 30 min at 50 ◦C. After the heating, the solution was transferred into a centrifuge tube and centrifuged for 5 min at 5000 rpm. The supernatant was collected, filled up to 50 mL with 80% methanol and kept at 4 ◦C for TPC, TFC and individual phenolics compounds determination.
