*2.14. Kinetics of the Yeast Growth Assay*

Yeast cells were grown in a liquid YPD medium (1% Difco yeast extract, 1% yeast bactopeptone, 2% (*w/v*) glucose) without (control) or with tested honeys or extracts on a rotary shaker at 150 rpm, or on a solid YPD medium containing 2% agar. The experiments were carried out at 28 ◦C. The growth was monitored using an Anthos 2010 type 17 550 microplate reader (Biochrom, Cambridge, UK) at 600 nm by measurements at 2 h intervals for 12 h. Each experiment was repeated at least three times.
