*3.3. Enzyme-Treated Bee Pollen Decreases the Production of IgE Antibodies in Mice Serum*

Generally, food allergy is an IgE-mediated type I hypersensitivity, which can induce immune cells to produce IgE antibodies [36–38]. Dot-blot assay was used to semiquantitatively analyze the level of IgE antibodies in mice serum. As shown in Figure 3A, OVA and BP treatment induced a significant increase in mice serum IgE levels. However, mice serum IgE levels in the 2E-BP and 3E-BP groups exhibited a notable decrease compared with that in BP group. This suggested that enzyme-treated bee pollen could decrease the production of IgE antibodies in mice serum due to a reduction in allergic reactions.

**Figure 3.** Changes in allergic mediators including IgE antibody (**A**), histamine (**B**), tryptamine (**C**), serotonin (**D**), spermine (**E**), spermidine (**F**) and octopamine (**G**) in mice serum of different treatment groups. Different letters indicate a significant difference among different groups (*p* < 0.05).
