*2.10. Antioxidant Capacity Assays*

Antioxidant capacity using DPPH and FRAP methods was determined according to procedures described by us previously [13]. The CUPRAC method was applied according to Matłok et al. [15]. Briefly, 10 μL of each diluted sample was pipetted into microplate wells, then 40 μL of CuCl2 (10 mM), 50 μL of neocuproine (7.5 mM), and 50 μL of ammonium

acetate (1 M) were added. The absorbance was measured with a microplate reader (EPOCH 2, BioTek, Winooski, VT, USA) at 450 nm after 30 min incubation in the dark against a blank. The result was expressed in Trolox equivalents (μmol TE/100 g) from the standard curve (y = 0.026x, r2 = 0.9973).
