*2.16. Bacteria Strain, Growth Condition, and Antibacterial Activity*

The antibacterial activity of the honey and extracts samples was assessed by monitoring the cell growth of a wide-use Escherichia coli DH5α strain (Thermo Fisher Scientific, Hennigsdorf, Germany). Bacterial cells were grown in a standard LB medium (LB Broth Lennox; BioShop, Burlington, Canada) without (control) or with tested honeys or extracts on a rotary shaker at 180 rpm. The growth was monitored using an Anthos 2010 type 17 550 microplate reader at 600 nm by measurements at 2 h intervals for 12 h. Each experiment was repeated at least three times. The experiments were carried out at optimal for bacteria 37 ◦C. The optical density of the culture was monitored every 2 h, at 600 nm wavelength.
