2.3.11. Determination of FT-IR

The bee pollen spectra in the wave number range of 4000–650 cm−<sup>1</sup> was recorded using a Nicolet i-20 spectrophotometer (Thermo Scientific, Waltham, MA, USA) with ATR module in absorbance mode. The bee pollen spectra were analysed using Spectra Gryph– spectroscopy software (Version 1.2.11). Each sample was ground into powder and filtered with 200 mesh, pressed and analysed directly on the ATR module. Each spectra was the media of 32 scans at a resolution of 4 cm−1. All the measurements were carried out in triplicate.
