*2.3. Sample Preparation*

The sample preparation for the quantitative method applied for the analysis of all the honey, pollen, and beebread samples of the current study was previously described by our group [11,16,17]. Briefly, 1 g of each matrix was spiked with the six internal standards, carbendazim-d3, imidachloprid-d4, dimethoate-d6, chlorpyriphos-d10, deltamethrin-d6, and triphenyl phosphate (TPP). The extraction step was performed by the application of a modified QuEChERS method using C18, PSA, and Z-Sep. The extract was then centrifuged (4500 rpm, 10 min), and the supernatant was collected, and the final extract was divided into two parts. The two aliquots were evaporated till dryness, and then they were reconstituted with 1 mL of a 75:25 (*v*/*v*) MeOH:H2O and pure acetone, respectively. The former aliquot was measured in LC-MS/MS, while the second in GC-MS/MS system.
