*2.7. A-Amylase Assay*

α-amylase was determined by a spectrophotometric method with the Phadebas Diastase test (Magle AB, Lund, Sweden) according to the manufacturer's instructions. The results of absorbance were measured at wavelength 620 nm against a blank (acetate buffer) using a Biomate 3 spectrophotometer (Thermo Scientific, Waltham, MA, USA). The results were calculated according to the formula attached in the manual and expressed as diastase number (DN).
