*2.2. Extracts Preparation*

Dried plant material was pulverized using pestle and mortar and weighted (5 g) into conical flasks. After flooding with 50 mL of 50% ethanol, it was extracted in an ultrasonic bath (Sonic 10, Polsonic, Warszawa, Poland) for 20 min, then filtered through a filter paper. Ethanol was evaporated using a centrifugal evaporator (VC 2–18 CDPlus, Martin Christ, Osterode am Harz, Germany) and then the aqueous residue was lyophilized using Alpha 1–2 LD plus freeze dryer (Martin Christ, Osterode am Harz, Germany).

For chromatographic analyses, honey samples were prepared using solid phase extraction (SPE). Twenty grams of each honey were dissolved in 100 mL of acidified distilled water (pH = 2) and passed through C-18 Sep Pack Cartridge (Waters Corporation, Milford, MA, USA), preconditioned with 10 mL of methanol and 10 mL of acidified (pH = 2) water. After washing the sugars with acidified water, the polyphenols were eluted with methanol (2.5 mL).
