*Article* **Botanical Origin of Galician Bee Pollen (Northwest Spain) for the Characterization of Phenolic Content and Antioxidant Activity**

**Sergio Rojo, Olga Escuredo \*, María Shantal Rodríguez-Flores and María Carmen Seijo**

Department of Vegetal Biology and Soil Sciences, Faculty of Sciences, University of Vigo, 32004 Ourense, Spain **\*** Correspondence: oescuredo@uvigo.es

**Abstract:** Bee pollen is considered a natural product, relevant for its nutritional and antioxidant properties. Its composition varies widely depending on its botanical and geographical origins. In this study, the botanical characteristics of 31 bee pollen samples from Galicia (Northwest Spain) were analyzed; samples have not been studied until now from this geographical area. The study focused on the evaluation of the influence of plant origin on total phenol and flavonoid contents and antioxidant activity measured by radical scavenging methods. The multivariate statistical treatment showed the contribution of certain pollen types in the extract of bee pollen as to phenols, flavonoids and antioxidant capacity. Specifically, the bee pollen samples with higher presence of *Castanea*, *Erica*, *Lythrum* and *Campanula* type indicated higher total phenol and flavonoid contents and antioxidant activities according to the principal component analysis. On the contrary, *Plantago* and *Taraxacum officinale* type contributed a lower content of these compounds and radical scavenging activity. The cluster analysis classified the bee pollen samples into three groups with significant differences (*p* > 0.05) for the main pollen types, total phenol and flavonoid contents and antioxidant capacities. These results demonstrate the richness and botanical diversity in the pollen spectrum of bee pollen and enhance the possible beneficial nutraceutical properties of this beekeeping product.

**Keywords:** *Apis mellifera* L.; bee pollen; botanical source; phenols; flavonoids; radical scavenging activity
