*2.3. Melissopalynological Analysis*

The pollen analysis of honey was performed according to the previously described procedure with slight modifications [39]. Briefly, ten grams of honey was dissolved in 50 mL distilled water at about 50 ◦C. The solution was then centrifuged for 8 min (3000 rpm) and, after removing the supernatant, 50 mL of distilled water was added to the sample again and the centrifugation was repeated. After the second centrifugation, the supernatant was not completely removed, leaving a tiny amount of solution above the sediment. The residue was mixed and placed on a microscope slide. Next, the slide was dried and secured with glycerol/gelatine and a coverslip. The collected material was analyzed using an Olympus BX41 microscope (Olympus America, PA, USA)) at 400× magnification. At least 300 consecutive pollen grains of nectar-producing plants were determined on each microscopic slide, and then the percentage content of *L. scoparium* pollen was calculated. Two repetitions were made for each honey sample and the final result is presented as an average of two results not differing between individual determinations by more than 5%, and rounded to the whole unit (%).
