**4. Conclusions**

In this study, we established that bee pollen is a rich source of protein, polyphenols, fatty acids, organic acids and amino acids. The organic acids (gluconic, lactic, acetic and propionic acids) provide antimicrobial properties for foods and are promoted as a new generation alternative to antibiotics, so bee pollen can be considered a potential preservation agent. The high protein content was observed in *Robinia pseudoacacia*, the high content of lipids was observed in *Robinia pseudoacacia* pollen, the high fructose content in *Prunus* spp. pollen while the high F/G ratio was observed in *Pinaceae* spp. pollen. The high TPC was observed in *Prunus* spp. pollen, the high TFC was observed in *Robinia pseudoacacia* pollen, the high free amino acid content was observed in *Pinaceae* spp. pollen, and the high content of PUFA was reported in *Taraxacum* spp. pollen. A total of 16 amino acids (eight essential amino acids and eight non-essential amino acids) were quantified in the bee pollen samples analyzed. Predominant were the unsaturated fatty acids (UFA; 62.65–927.50 μg/g), of which levels of 7.51–88.69 μg/g were determined for monounsaturated fatty acids (MUFAs) and 43.86–838.82 μg/g for polyunsaturated fatty acids (PUFAs). According to the data obtained, bee pollen can be considered a complex matrix with a high potential as food supplement or source of bioactive compounds for the pharmaceutical industry.

**Author Contributions:** Conceptualization, F.U.; data curation, F.D.; formal analysis, M.O.; funding acquisition, M.O.; investigation, M.O. and F.U.; methodology, F.D. and F.U.; project administration, M.O.; resources, F.D.; software, F.D.; validation, M.O. and F.U.; writing—original draft, F.D.; writing review and editing, M.O. All authors have read and agreed to the published version of the manuscript.

**Funding:** This work was funded by Ministry of Research, Innovation and Digitalization within Program 1—Development of national research and development system, Subprogram 1.2—Institutional Performance—RDI excellence funding projects, under contract no. 10PFE/2021.

**Data Availability Statement:** Data is contained within the article.

**Conflicts of Interest:** The authors declare no conflict of interest.
