*2.7. Diastase Activity*

Diastase activity was calculated according to the AOAC method 958.09 [34]. The honey sample (5 g) was diluted in 10 mL of deionized water and 2.5 mL of acetate buffer (1.59 M, pH 5.3). The diluted sample was then transferred to a 25 mL volumetric flask containing 1.5 mL of 0.5 M NaCl solution. Ten ml of honey solution was mixed with 100 mL of 1% (*w*/*v*) starch solution and incubated in a water bath at 40 ◦C for 5 min. After that, 1 mL of the treated sample was mixed with 10 mL of 0.0007 M diluted iodine solution and measured at 660 nm in a spectrophotometer (Thermo-scientific Evolution 60S model, Waltham, MA, USA).
