*2.11. Determination of Water Holding Capacity (WHC)*

All samples were cut into 3 g with a thickness of about 5 mm, weighed (W1) and then placed into three layers of filter paper. After centrifugation of 15 min (5000× *g*, 4 ◦C), the gels were quickly weighed again (W2). The equation of water holding capacity is defined as follows:

$$\text{Centrifugal loss} \left( \% \right) = \frac{W\_2}{W\_1} \times 100 \tag{3}$$

$$\text{Water holding capacity} \left(\% \right) = \frac{\text{M} - \text{CL}}{\text{M}} \times 100 \tag{4}$$

where CL is the centrifugal loss of surimi gel after heating, and M is the moisture content determined by drying the sample at 105 ◦C until constant weight.
