**4. Conclusions**

The polysaccharides from *H. leucospilota* were utilized and decomposed into free monosaccharides by the intestinal microbiota. During in-vitro human fecal fermentation, the pH values and total sugar contents decreased, while various SCFA contents increased. After 28 days of high-dose treatment, the acetic and isobutyric acid levels in mouse feces increased significantly. Additionally, HLP exhibited antioxidant activity by enhancing the GSH-Px and SOD levels and decreasing the MDA contents in mouse livers. This study suggested that HLP plays an important role in potential health benefits by promoting intestinal health and antioxidant activity.

**Author Contributions:** Conceptualization, C.L.; methodology, W.W. and J.C.; software, Y.Y. and J.C.; formal analysis, Y.Y.; investigation, Y.Y.; resources, C.L.; writing—original draft preparation, W.W.; writing—review and editing, C.L.; supervision, J.C. and C.L.; project administration, X.S. All authors have read and agreed to the published version of the manuscript.

**Funding:** This research was funded by the Hainan Provincial Key Research and Development Program, grant number ZDYF2020170.

**Institutional Review Board Statement:** The study was conducted according to the guidelines of the Care and Use of Laboratory Animals published by the U.S. National Institutes of Health. All methods and materials in the study were granted Ethical approval from Animal Experimentation Ethics Committee of Hainan Medical University. (No. HYLL-2019-038; 21/09/2019).

**Data Availability Statement:** All raw data supporting reported results is available from authors upon request.

**Conflicts of Interest:** The authors declare that they have no conflict of interest.
