**4. Conclusions**

In summary, different ultrasonication and homogenization treatments combination on zein and FSG can significantly influence the properties of the complexes and the emulsion formed by the complexes. Ultrasonic treatment affected the interaction between ZNP and FSG. The complex formed by ZNPU and FSG has the highest absolute zeta potential value, better emulsification properties (higher EAI and ESI), and the highest surface hydrophobicity among all, which indicates that the complex was stable and can be used as a good Pickering emulsions stabilizer among those complexes subjected to various ultrasound and homogenization treatments. However, the properties of (ZNP-FSG)U and (ZNP-FSG)HU are very different from those of (ZNPU-FSG)H. (ZNPU-FSG)H-E with good storage stability and freeze-thaw stability can be observed in the microscope with a clear, regular, and uniform spherical structure. In conclusion, these results indicate that the timing of using ultrasound in the preparation of complexes and emulsions is very important. These results provide new ideas and a theoretical basis for improving the properties of protein–polysaccharide complexes and emulsions.

**Supplementary Materials:** The following are available online at https://www.mdpi.com/article/10 .3390/foods10091990/s1, Figure S1: The particle size distribution of 9 various ingredients or ZNP -FSG complexes subjected to various ultrasound and homogenization treatments.

**Author Contributions:** Conceptualization, Y.L.; data curation, Y.L.; methodology, Y.L., G.X., W.L. and L.L.; investigation, Y.L. and Q.Z.; software, Q.Z., W.L. and L.L.; writing—original draft preparation, Y.L. and Q.Z.; writing—review and editing, Q.Z.; visualization, Y.L. and G.X.; All authors have read and agreed to the published version of the manuscript.

**Funding:** This study was financially supported by National Natural Science Foundation of China (grant numbers 31701608) and the Natural Science Research Projects of Jiangsu Higher Education Institution (grant numbers 17KJD550002).

**Institutional Review Board Statement:** Not applicable.

**Informed Consent Statement:** Not applicable.

**Data Availability Statement:** The datasets generated for this study are available on request to the corresponding author.

**Acknowledgments:** The authors are grateful for the financial supports from National Natural Science Foundation of China (No. 31701608). The authors would like to thank Huijuan Zheng for her assistance.

**Conflicts of Interest:** The authors declare no conflict of interest.

#### **References**

