*3.7. ROS Production in H2O2-Induced IEC-6 Cells*

Excessive ROS production can damage cells and lead to cellular oxidative stress, which can cause the development of various diseases [6]. H2O2 significantly increased the ROS level in IEC-6 cells to 3.1-fold (Figure 4C) compared with the control group. The high dose (800 μg/mL) of CYP significantly reduced the release of intracellular ROS. Interestingly, this effect was higher when compared to control. Moreover, pretreatment of IEC-6 cells with different CYP concentrations reduced the level of ROS in a significant dose-dependent manner from 200 μg/mL to 800 μg/mL. These results were similar to those of Liao et al. [7].
