*2.3. Primer Design*

Multiplex PCR primers were designed using the MultiplexSSR pipeline, as described by Guo and Yang [26]. The resequencing data from 12 randomly selected individuals were processed using this pipeline. The amplicons had a minimum length of 80 bp, maximum length of 480 bp, and minimum spacing of 20 bp. The minimum length of repeat units, minimum number of genotyped individuals, minimum number of alleles, and minimum depth of genotypes were set to 3, 5, 5, and 1, respectively. The optimum annealing temperature was set at 60 ◦C.
