**2. Materials and Methods**

### *2.1. Experimental Animals*

Examples of *P. monodon* were procured from the Experimental Base of South China Sea Fisheries Research Institute in Shenzhen. The shrimp with a body length of 12.2 ± 0.6 cm and a weight of 16.2 ± 1.1 g were used in experiments. They were refined in sifted circulated air through seawater at 28 ± 1 ◦C, salinity 25–28 psu, pH 7.8–8.2, and fed business feed.
