*Article* **Dietary Curcumin Supplementation Enhanced Ammonia Nitrogen Stress Tolerance in Greater Amberjack (***Seriola dumerili***): Growth, Serum Biochemistry and Expression of Stress-Related Genes**

**Jiawei Hong 1,2,3, Zhengyi Fu 1,2, Jing Hu 1,2, Shengjie Zhou 1,2, Gang Yu 1,2 and Zhenhua Ma 1,2,\***


**Abstract:** This study was conducted to determine whether curcumin has a positive effect in greater amberjack (*Seriola dumerili*), especially the ammonia nitrogen stress tolerance ability. The results showed that the stress recovery process of digestive enzymes amylase and trypsin, as well as absorptive enzymes Na+/K+-ATPase, γ-GT and CK, was accelerated. Lysozyme activity increased in the fish fortified with both curcumin diets. Aspartate aminotransferase activity restriction was activated at a low curcumin level. However, alanine aminotransferase activity restriction happened only at 0.02% dietary curcumin. Facilitation of lipid metabolism by curcumin was very clear, as triglyceride and total cholesterol content was basically maintained at the original level or even showed a slight decrease after recovery. HSP70 and HSP90 genes were not evidently stimulated to express in liver, kidney and spleen tissues. In addition, curcumin showed its inhibition capacity on IL1β and IFN-γ and a promoting effect on TGF-β1. The expression of NF-κB1 decreased in a higher degree in fish fed with 0.02% dietary curcumin, while 0.01% dietary curcumin accelerated the recovery pace of C3 and lgT after stress. This study showed that dietary curcumin supplementation can enhance ammonia nitrogen stress tolerance in greater amberjack, and its application prospect can be confirmed.

**Keywords:** *Seriola dumerili*; curcumin; ammonia nitrogen tolerance
