*Article* **Growth and Biochemical Composition of Microgreens Grown in Different Formulated Soilless Media**

**Roksana Saleh 1, Lokanadha R. Gunupuru 1, Rajasekaran Lada 1, Vilis Nams 1, Raymond H. Thomas <sup>2</sup> and Lord Abbey 1,\***


**Abstract:** Microgreens are immature young plants grown for their health benefits. A study was performed to evaluate the different mixed growing media on growth, chemical composition, and antioxidant activities of four microgreen species: namely, kale (*Brassica oleracea* L. var. *acephala*), Swiss chard (*Beta vulgaris* var. *cicla*), arugula (*Eruca vesicaria* ssp. *sativa*), and pak choi (*Brassica rapa* var. *chinensis*). The growing media were T1.1 (30% vermicast + 30% sawdust + 10% perlite + 30% PittMoss (PM)); T2.1 (30% vermicast + 20% sawdust + 20% perlite + 30% PM); PM was replaced with mushroom compost in the respective media to form T1.2 and T2.2. Positive control (PC) was Pro-mix BX™ potting medium alone. Root length was the highest in T1.1 while the shoot length, root volume, and yield were highest in T2.2. Chlorophyll and carotenoid contents of Swiss chard grown in T1.1 was the highest, followed by T2.2 and T1.1. Pak choi and kale had the highest sugar and protein contents in T2.2, respectively. Consistently, total phenolics and flavonoids of the microgreens were increased by 1.5-fold in T1.1 and T2.2 compared to PC. Antioxidant enzyme activities were increased in all the four microgreens grown in T1.1 and T2.2. Overall, T2.2 was the most effective growing media to increase microgreens plant growth, yield, and biochemical composition.

**Keywords:** microgreens; natural amendment; soil health; phytochemicals; healthy food
