*4.12. Antioxidant Activity*

The free radical scavenging capability of the film samples was determined by using the DPPH and ABTS radical scavenging activity. The antioxidant activity of DPPH was studied as per the procedure described earlier by Brand-Williams et al. [35], utilizing 50 mg of film samples. The measurement of absorbance was conducted at a wavelength of 517 nm utilizing an ONDA Vis spectrophotometer. The outcomes were expressed as a percentage of inhibition. The antioxidant potential was assessed by using the chromogenic compound ABTS as per the procedure followed by Re et al. [36], with some minor modifications. The sample amount of 25 mg was used in the ABTS assay. The measurement of absorbance was conducted at a wavelength of 734 nm. The outcomes were expressed as a percentage of inhibition and were derived from an average of three measurements.
