*4.11. Antioxidant Analysis*

The antioxidant potential of the SA–AG-based films was analyzed using DPPH and ABTS radical scavenging activities. For the DPPH assay, the method described by Brand-Williams et al. [49] was followed to carry out the analysis for 50 mg of film samples. The absorbance was recorded at 517 nm using an ONDA-Vis spectrophotometer. The obtained results were presented as % inhibition. For the ABTS assay, the methodology of Re et al. [50] was employed to evaluate the antioxidant activity of the 25 mg of film samples. The absorbance was recorded at 734 nm and the results that were obtained were presented as % inhibition as an average of three measurements. The films were directly treated with radical solutions, vortexed for 30 s and incubated for radical scavenging activity.
