*4.9. Statistical Analysis*

The study was replicated three times. Data were analyzed using the SPSS software (IBM SPSS statistics 21). Mean values of different parameters were used to compare chemical and microbiological indices. Sensory attributes data were analyzed by analysis of variance (ANOVA). The mean values ± standard deviation (SD) of the analyses were calculated. When a significant main effect was detected, the means were separated with the least significant difference (LSD) procedure. A two-way analysis of variance was used for multiple variable comparisons. Using analysis of variance (ANOVA), Tukey's test, and independent sample *t*-test for all data interpretation at *p* < 0.05.

**Supplementary Materials:** The following supporting information can be downloaded at: https: //www.mdpi.com/article/10.3390/gels8070405/s1, Table S1: Effect of storage conditions (2 ◦C for 20 days) and treatments on sensory evaluation during storage of mackerel tuna fillets.

**Author Contributions:** Conceptualization, A.F.E.S.; methodology, A.Y.A.; software, A.Y.A.; validation, A.F.E.S. and F.O.; formal analysis, A.Y.A.; data curation, A.F.E.S.; writing—review and editing, A.F.E.S., E.O., M.R.K., C.P. and F.O.; supervision, A.F.E.S.; funding acquisition, A.F.E.S. and M.R.K. All authors have read and agreed to the published version of the manuscript.

**Funding:** This research was supported by the Researchers Supporting Project No. (RSP-2021/138) King Saud University, Riyadh, Saudi Arabia. This research was also supported by the Research Start-Up Fund from Jiangxi Agricultural University, China (grant number: 9232307245) and SPECIAL ACCOUNT FOR RESEARCH GRANTS of the National and Kapodistrian University of Athens, Greece (grant number: 15363).

**Institutional Review Board Statement:** Not applicable.

**Informed Consent Statement:** Not applicable.

**Data Availability Statement:** The data presented in this study are available on request from the corresponding author.

**Acknowledgments:** The authors thank to the Jiangxi Agricultural University (China), Minufiya University (Egypt), Ataturk University (Turkey), King Saud University (Saudi Arabia), and National and Kapodistrian University of Athens (Greece), UoA/S.A.R.G. The authors would like also to thank all staff in these universities for their intellectual and research support.

**Conflicts of Interest:** The authors declare no conflict of interest.
