2.1.11. Cell Culture

The MCF-7, MDA-MB 231 and T47D cell lines were cultured in RPMI 1640 growth medium, supplemented with 5% fetal bovine serum, 2 mM L-Glutamine, 100 U mL−<sup>1</sup> penicillin and 100 μg mL−<sup>1</sup> streptomycin. Cells were maintained at 37 ◦C in a humidified 5% CO2 incubator. ER(+) human breast cancer cell line MCF7, ER/PR (+) human breast cancer cell line T47D and triple negative human breast cancer (TNBC) cell line MB231 were purchased from NCI (NCI, NIH, Frederick, MD, USA).

### *2.2. Synthesis of the Monomers*
