*2.7. Load and Realese of Ciprofloxacin*

### 2.7.1. Ciprofloxacin Load

Samples around 100 mg were placed in vials with 3 mL of 0.012 μg/mL ciprofloxacin aqueous solution, at 25 ◦C, for 30 h. The loading time was determined by measuring the absorbance at different time intervals to assess the loading progress (4, 6, 8, 24, and 30 h). The amount of drug loaded was determined by measuring the difference in absorbance between a 0.012 μg/g ciprofloxacin solution without material and the solution with the material at each time interval at a wavelength of 266 nm, using a calibration curve. A SPECORD 200 PLUS brand spectrophotometer from Analytikjena (Germany) was used for the test. After that time, the samples were extracted and gently washed with distilled water. The calibration curve is shown in Supplementary Materials, in Figure S1.
