**4. Conclusions**

This study examined the occurrence of 15 pharmaceutically active compounds (PhACs), 18 antibiotics, and one stimulant (caffeine) in influent and effluent from Assamra WWTP and in water samples from the Zarqa River and its tributaries. Based on the results obtained, the following conclusions were drawn:


**Supplementary Materials:** The following are available online at https://www.mdpi.com/2076-3 417/11/18/8638/s1, Table S1. Geographic positioning system (GPS) coordinates of the sampling sites for wastewater treatment plant (WWTP) influent and effluent and Zarqa River (ZR) water. Data from [35], Table S2. List of pharmaceutically active compounds (PhACs, including antibiotics) targeted in analysis of liquid samples and their chemical and physical properties (chemical formula, molecular weight (MW), logarithmic octanol-water distribution coefficient (log Kow), organic carbonwater partition coefficient (Koc), logarithmic dissociation constant (pKa), and water solubility at 25 ◦C (mg/L). Values without any reference superscript (+) were modeled and taken from ChemSpider (2020), Table S3. Concentrations of total suspended solids (TSS), total solids (TS) and chemical oxygen demand (COD) samples (n = 1 per site) in Assamra wastewater treatment plant (WWTP) influent and effluent and Zarqa River (ZR) water at Sukhna station, Twahin Eledwan station, Military station, and Jerash stream. Data from [35].

**Author Contributions:** The authors had contributed to the published work according to the following description: M.S.: conducted the laboratory analyses, the statistical analyses and revised the manuscript according to reviewers input; A.A.: participated in design and planning of the study; A.H.: participated in describing and visualizing the study area, S.S.D.: planned the study, collected the samples, participated in data analyses and wrote the manuscript. All authors have read and agreed to the published version of the manuscript.

**Funding:** The study was funded by the Swedish Research Council (FORMAS) through grant 169- 2013-1963 for the project "Pharmaceutical Pollution at Use of Wastewater in Crop Production" and the Swedish Foundation for international cooperation in research and education (grant number PT2016–6875).

**Data Availability Statement:** Original data are presented in the supporting information.

**Acknowledgments:** Special thanks to Raed Jaber, Samir Tawalbeh, and Mohamed Mashatleh at the Royal Scientific Society of Jordan for their assistance in sample collection and facilitating the shipping of samples to Sweden. Thanks also to members of the Water Quality Laboratory at the Royal Scientific Society of Jordan for their efforts in physical and chemical analyses of the samples. Instrumental identification of the pharmaceuticals was performed at the Swedish Environmental Institute (IVL), Stockholm. Special thanks to Gunnar Thorsén for his effort in the analytical procedures.

**Conflicts of Interest:** The authors have no competing interest to declare, or financial or personal relationships with other people or organizations that could have inappropriately influenced this work.

#### **References**

