*2.1. Sample Description*

The AL used for this work corresponds with the semi-solid effluent generated during olive oil extraction by a two-phase extraction system. The sample was collected from an olive oil factory sited in Sevilla, Spain. For the fungal treatment, the sample was mixed with distilled water in a ratio 1:20 (*p*/*v*). The mix was filtered using a 1.5 mm mesh sieve to remove the rest of the peel and pit of the olives. After that, the effluent was centrifuged for 10 min at 9000× *g* and the supernatant was filtered by a cellulose filter (10–20 μm). This diluted AL was used for the subsequent fungal treatments. The characteristics of diluted AL are shown in Table 1.

**Table 1.** Characteristics of the diluted AL.

