*2.6. LC50 Calculation and Statistical Analyses*

Toxicity data were reported as mean ± standard deviation (SD). Data were checked for normality using the Shapiro–Wilk's (S–W) test (*p*-value < 0.05). The significance of differences among treatments and the control was checked by two-way ANOVA followed by post hoc Tukey's test for multiple comparisons (GraphPad Prism Software version 8.02 for Windows, GraphPad Software, La Jolla, CA, USA, www.graphpad.com, accessed on 1 February 2021). *p*-Values < 0.05 were considered statistically significant. The calculation of LC50 values was done by GraphPad Software through four parameters of the logistic equation, which corresponds to the dose-response curve with the slope of the variable slope.
