*2.2. Zebrafish Maintenance and Breeding*

All zebrafish research procedures were approved by the Ethical Committee of Ningbo University. The AB strain adult zebrafish (*Danio rerio*) (Shanghai FishBio Co., Ltd., Shanghai, China) were maintained in a light/dark cycle of 14/10 h. The zebrafish were fed with freshly hatched brine shrimp twice daily. Water quality was monitored every day to ensure pH 7.3–7.4, conductivity within 450–500 μS, and a temperature of 28 ± 1 ◦C. Male and female (2:2) zebrafish were placed in isolation in the mating tanks in order to trigger spawning when the next morning lights on. After two hours, the normal embryos were collected and cultivated in 1 × EM medium at 28 ◦C.
