*Article* **Study of Ultrastructural Abnormalities in the Renal Cells of** *Cyprinus carpio* **Induced by Toxicants**

**Sumayya Nazir 1,\*, Md. Niamat Ali 2,\*, Javeed Ahmad Tantray 3, Irfan Akram Baba 4, Arizo Jan 5, Simona Mariana Popescu 6, Bilal Ahamad Paray <sup>7</sup> and Aneela Gulnaz <sup>8</sup>**


**Abstract:** Transmission Electron Microscopic (TEM) assessments were performed on the renal cells of common carp *Cyprinus carpio* to observe the deleterious effects of two organophosphate insecticides, Phorate and Dimethoate. Pesticides such as Phorate and Dimethoate often pollute aquatic systems where they may negatively impact fish, but so far, the ultrastructural toxicity of these pesticides remains poorly understood. Here, we use Transmission Electron Microscopy (TEM) to determine how acute exposure to sublethal concentrations of these two pesticides may affect the renal cells of common carp *Cyprinus carpio*. For each insecticide, the fish were divided in four experimental conditions: a control and three different exposure concentrations of the pesticide. The Phorate treated fish were exposed to three sublethal concentrations of 0.2 mg/L, 0.4 mg/L, 0.6 mg/L for a duration of 24, 48 & 72 h. The dimethoate treated fish were exposed to three sublethal concentrations of 0.005 mL/L, 0.01 mL/L, 0.015 mL/L for a duration of 24, 48 and 72 h. The two-dimensional transmission electron microscopy revealed ultrastructural abnormalities in the treated fish renal cells when exposed to two toxicants including deformation in the glomerulus, vacuolization of cytoplasm, degenerative nucleus and damaged mitochondria. Furthermore, the ultrastructural abnormalities were more prominent with the increase in the concentrations of both the insecticides and also with their exposure period. Overall, these results provide important baseline data on the ultrastructural toxicity of Phorate and Dimethoate and will allow important follow-up studies to further elucidate the underlying cellular mechanisms of pesticide toxicity in wildlife.

**Keywords:** Phorate; dimethoate; TEM; renal cells; ultrastructure; toxicants
