*2.7. Statistical Analysis and Graphs*

All graphs were plotted using GraphPad Prism 7.0 software (GraphPad Software Inc., San Diego, CA, USA). For linearity, homoscedasticity analysis was carried out, and the R square was found. The limit of detection (LOD) and the limit of quantification (LOQ) were determined by the signal–noise method, and five blanks were analyzed by uHPLC– DAD via the method presented above. Finally, the ED effects, due to changes in β-hCG and sperm, were defined using case–control methods via the application of an unpaired *t*-test analysis.
