*3.1. Exposure Concentrations*

To determine the TFZ dose which caused harmful effects in zebrafish, different concentrations were used for exposure and survival rates were recorded. For the primary screening of tolerance ranges in 3 dpf zebrafish embryos and 6 dpf zebrafish larvae, we used 0, 30, 60, 90, 120, and 150 mg/L TFZ. Next, we exposed 3 dpf zebrafish embryos and 6 dpf zebrafish larvae to 0, 1, 2, 3 mg/L and 0, 0.5, 1, 1.5 mg/L TFZ, respectively (Figure 1A,B). After TFZ exposure, the 48 h LC50 of 3 dpf zebrafish embryos is 4.872 mg/L (Figure 1C), and the 24 h LC50 of 6 dpf zebrafish larvae is 2.580 mg/L (Figure 1D).
