*2.2. Culture Medium and Culture Conditions*

The fresh cultured *V*. *qinghaiensis* Q67 was used for the measurement of chronic toxicity. The medium for the Q67 contained tryptone (5 g/L), yeast extract (5 g/L), MgCl2 (3.2 g/L), KBr (0.2 g/L), CaSO4 (0.1 g/L), KCl (4 g/L), NaCl (4 g/L), and glycerol (3 mL/L), with the pH adjusted to 8.5 [50]. *V*. *qinghaiensis* Q67 was cultured at 20 ◦C for 12 h with 180 rpm shaking; then, the harvested fresh culture was used to test the chronic toxicity.
