**5. Conclusions**

In summary, our results show TFZ-induced morphological changes (including pericardium edema, yolk retention, and liver degeneration) as well as death in zebrafish embryos. TFZ exposure could cause oxidative stress in zebrafish larvae. Moreover, the upregulation of *ppar-γ* suggests that the PPAR signaling pathway might participate in the imbalance of lipid metabolism. These findings provide a new insight into the potential mechanisms underlying the lipid metabolism imbalance of TFZ in zebrafish.

**Supplementary Materials:** The following supporting information can be downloaded at: https://www. mdpi.com/article/10.3390/toxics10110698/s1, Table S1: The sequence of primers for qPCR [42–51].

**Author Contributions:** L.B.: Methodology, investigation, writing. P.S.: cell culture, software, formal analysis, data curation. K.J.: methodology, manuscript-revised. H.Y.: resources. K.L.: conceptualization, methodology, manuscript-finalized, supervision, project administration, funding acquisition. J.X.: supervision, resources. X.Y.: resources. All authors have read and agreed to the published version of the manuscript.

**Funding:** This study was funded by the National Natural Science Foundation of China (grant number: 42076118 and 41706159), the Key Research Foundation of Ningbo No.2 Hospital, China (grant number: 2022HMZD08) and partly sponsored by K.C. Wong Magna Fund in Ningbo University.

**Institutional Review Board Statement:** The study was conducted in accordance with guidelines for the care and use of laboratory animals, as approved by the Ethical Committee of Ningbo University.

**Informed Consent Statement:** Not applicable.

**Data Availability Statement:** Data are contained within the article or Supplementary Materials.

**Acknowledgments:** We sincerely thank G.C.Y. and L.Z. for maintaining the zebrafish in a healthy condition.

**Conflicts of Interest:** The authors have no conflict of interest.
