2.4.2. Acute and Chronic Tests on *D. magna*

The acute toxicity test on *D. magna* for both ELT-dg and ELT-dp suspensions was conducted in a 48 h exposure under static conditions to 100.0 mg/L [48]. In particular, we evaluated the immobilization, defined as organisms not able to swim within 15 s after gentle agitation. Specimens (daphnid age < 24 h) obtained by ChemService Controlli e Ricerche s.r.l. breeding were exposed in 40 mL beakers with pre-aerated (oxygenation > 5.62 mg/L) reconstituted water (see Supplementary Materials for composition), with 4 replicates *per* treatment (5 daphnids *per* beaker) at 20 ◦C, with 16 h of light and 8 h of darkness (1188–1401 lx). The immobilization was checked after 24 and 48 h. The chronic test on *D. magna* for both ELT-dg and ELT-dp was conducted in 21 days of exposure in semi-static condition (renewal 3 times *per* week) to 1.0, 3.1, 9.8, 31.3 and 100.0 mg/L [50]. In this context, after 21 days of exposure, we evaluated the mortality rate and reproduction parameters (total number of living offspring). Specimens (daphnid age < 24 h) were exposed in 50 mL beakers with pre-aerated (oxygenation > 5.00 mg/L) reconstituted water (see the Supplementary Materials for the composition) with 10 replicates *per* treatment (10 daphnids *per* treatment, 1 specimen *per* beaker) at 20 ◦C, with 16 h of light and 8 h of darkness (1236–1467 lx). During exposure, daphnids were feed with *P. subcapitata* after the water change.
