*4.2. Cell Viability*

For the MTS assay, cells were plated in triplicate at 1.0 × <sup>10</sup><sup>4</sup> cells/well in 96-well culture plates in culture media. At 24 h after plating, CellTiter 96 AQueous One Solution reagent (Promega, Madison, WI, USA) was added to each well according to the manufacturer's instructions, and absorbance at 490 nm was determined for each well using a Victor 1420 Multilabel Counter (EG&G Wallac, Turku, Finland).

#### *4.3. Plasmids, Small Interfering RNAs, Transfections, and Dual-Luciferase Assay*

The pGL3/TTPp-1343 plasmid containing the human *TTP* promoter was described previously [11]. The pGR-wt, pGRdim, and pFOXO1-Flag plasmids were purchased from Addgene (Watertown, MA, USA).

Small interfering RNAs (siRNAs) against human *TTP* (TTP-siRNA, sc-36761) and control siRNA [scrambled siRNA (scRNA), sc-37007] were purchased from Santa Cruz Biotechnology (Santa Cruz, Santa Cruz, CA, USA). Cells were transfected 24 h after plating using LipofectamineTM RNAiMAX (Invitrogen, Carlsbad, CA, USA) and harvested at 48 h after transfection. The expression levels of *TTP* mRNA were analyzed by qRT-PCR.
