*2.6. Genistein's Inhibition of Cell Migration in the Wound*−*Healing Assay*

We also assessed cell migration activities using a wound-healing assay. As shown in Figure 8A,B, the continuous migration of HeLa cells was observed in the control group after 8 h and 24 h. HeLa cell migration was significantly reduced in the presence of 12.5–100 μM genistein. The results indicate that genistein strongly inhibits cell migration in a concentration-dependent manner (*p* < 0.01; *p* < 0.05).

**Figure 8.** Effect of genistein on the mobility of HeLa cells. (**A**) Representative images of woundhealing assay. (**B**) Cell migration was calculated by measuring the distances from the wound edges in each treatment group using Image J software. Cell migration activity (as a percentage) was calculated from the migration distances in the genistein treatment group (compared to the control (100%)). The experiment was repeated three times. \*\* *p* < 0.01 vs. DMSO control. \* *p* < 0.05 vs. DMSO control; Scale bar: 200 μm.
