*4.24. Cell Culture*

Breast cancer cell lines were obtained from the Department of Radiobiology (MLU Halle-Wittenberg) and previously described. MDA-MB-231, HS578T and MCF-7, and T47D were cultured as a monolayer in RPMI (Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal bovine serum (Capricorn Scientific, Ebsdorfergrund, Germany), 2% penicillin/streptomycin (Sigma-Aldrich, St. Louis, MO, USA), and 1% sodium pyruvate (Gibco, Thermo Fisher Scientific) at 37 ◦C and 5% CO2. All cell lines were regularly tested for mycoplasma contamination.
