**Animals**

All experiments were performed in accordance with procedures approved by the Pennsylvania State University College of Medicine Institutional Animal Care and Use Committee (protocol 202001327). Wild-type C57BL/6 (The Jackson Laboratory, Bar Harbor, ME, USA) male mice (*n* = 35) were used for all experiments. Animals were grouped housed with a 12-h light/dark cycle with *ad libitum* food and water.

Cisplatin-induced peripheral neuropathy was induced as previously described [13,15,34]. Mice were injected with 4% sodium bicarbonate solution (1 mL, s.c.) administered just prior to cisplatin (5 mg/kg, i.p.) to prevent neurotoxicity and to minimize damage to renal function. Cisplatin was administered once a week for four weeks [15,35]. Mechanical allodynia was assessed before and after cisplatin treatment to confirm neuropathic pain state as described below.

Mechanical hypersensitivity was assessed using an electronic von Frey anesthesiometer (IITC Life Sciences Inc., Woodland Hills, CA, USA) with a semi-flex tip (IITC Life Sciences Inc., Woodland Hills, CA, USA), which was applied to the plantar surface of the right hind-paw with increasing force to prompt a paw withdrawal response. Mice were placed in one of eight small acrylic chambers placed on a wire mesh table (IITC Life Sciences

Inc., Woodland, CA, USA), and acclimated to the chamber for 20 min before testing. The average of three tests were calculated with each test separated by at least 3 min.

To measure the effects of test compounds, 35 neuropathic male mice were randomly assigned to 7 groups (5 mice/group). During week 1, mice were injected with vehicle (DMSO, Tween 80, saline (1:1:18), i.p.), CBGV, CBGB, CBG, CBGP, CBGN, or the nonsteroidal anti-inflammatory drug indomethacin (10 mg/kg, i.p., based upon our previous work with CBG [15]). Mice were then given a one-week washout period, and during week 2 were again randomly assigned to one of the 7 groups and injected with the test compounds. All von Frey experiments were performed by experimenters blinded to treatments.
