**2. Materials and Methods**

#### *2.1. Main Reagents*

Scabertopin (PS1863-0010) was purchased from Chengdu Push Biotechnology Co., Ltd. (Chengdu, China). DMEM basal medium (10569010) and trypsin-EDTA (25200056) were purchased from Gibco (Grand Island, NY, USA). RPMI 1640 (PM150110) was purchased from Procell Life Science & Technology Co., Ltd (Wuhan, China). Fetal bovine serum (FBS) (1907301) was purchased from Biological Industries (BI) (Kibbutz Beit Haemek, Israel). Cell Counting Kit-8 (CK04) was purchased from Dojindo Laboratories (Kumamoto, Japan). LIVE/DEAD cell viability assay kit (L3224) was purchased from Invitrogen (Carlsbad, CA, USA). The cell cycle assay kit (KGA512) and the apoptosis assay kit (KGA1018) were purchased from KeyGen BioTECH (Shanghai, China). ROS detection kit (S0033S) was purchased from Beyotime Biotechnology (Shanghai, China). B cell lymphoma-2 (Bcl-2, ab182858), Bcl-2-associated X (Bax, ab32503), phospho-PI3K (Y607, ab182651), AKT (ab179463), phospho-AKT (S472, S473, S474, ab192623), and GAPDH (ab181602) antibodies were purchased from Abcam (Cambridge, MA, USA). Gasdermin D (97558), GPX4 (59735), FAK (71433), phospho-FAK (Y397, 8556), MMP-9 (13667), PI3K (4257S), caspase-3 (14220), caspase-8 (9746), caspase-9 (9508), MLKL (14993), phospho-MLKL (S358, 91689), RIP1 (3493), phospho-RIP1 (Ser166, 65746), RIP3 (13526), and phospho-RIP3 (Ser227, 93654) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA).
