*3.9. Western Blotting Analysis*

Whole-cell lysates were prepared, and Western blotting was performed as previously described ([31]). Primary antibodies against STAT3, p-STAT3 (Tyr705), JAK2, Src, p-Src (Tyr416), CDK4, CDK6, and p-CDK1 (Tyr15) were purchased from Cell Signaling Technology (Danvers, MA, USA). Additionally, p-JAK2 (Tyr1007/1008), CDK1, cyclin B1, PARP, SHP1, SHP2, and β-actin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA), and HRP-conjugated anti-mouse IgG and anti-rabbit (Cell Signaling Biotechnology) were used. Densitometric analysis of each band was performed using the ImageJ software (https://imagej.nih.gov/ij accessed and downloaded the updated software on 27 September 2021). Data are presented as the mean ± standard deviation (SD) from at least three independent experiments.
