*4.1. Reagents*

All cultures and chemicals were purchased from Nacalai Tesque (Kyoto, Japan) unless otherwise indicated. Fetal bovine serum (FBS) was purchased from Bio West (Nuaillé, France). Bortezomib (a protease inhibitor) was obtained from Wako Chemicals (Osaka, Japan). TP and TOS were purchased from Sigma Aldrich (St. Louis, MO, USA), and T3 was purchased from Tama Biochemicals (Tokyo, Japan). Antibodies for NFE2L1 (#8052), ubiquitin (#3936) and Lamin B1 (#13435) were purchased from Cell Signaling Technology

(Beverly, MA, USA). DDI2 antibody (sc-514004) is purchased from Santa Cruz Biotechnology (Dallas, TX, USA).
