*4.3. Cell Cycle Analysis*

LCLs and DLBCLs cells were seeded in plates (5 × <sup>10</sup><sup>5</sup> cells/well) for 24 h and then treated with 100 μg/mL of native fucoidan or F1/F2 fractions. After 48 h, cells were collected, washed twice in Dulbecco's Phosphate Buffered Saline (DPBS–Eurobio Scientific) and fixed with ice-cold 70% ethanol overnight. For Propidium Iodide (PI–Sigma Life Sciences) staining, fixed cells were washed twice with cold DPBS and incubated in 30μL of RNase working solution (10 mg/mL) and 1 mL cold DPBS for 20 min at room temperature (RT). Then, samples were stained with PI and analyzed using a BD FACSCalibur flow cytometer and Kaluza Analysis 2.1 Software (Beckman Coulter).
