*4.2. α-T3E Synthesis*

α-T3E was synthesized from T3 according to a previously reported procedure [39]. The purity of α-T3E was confirmed by GC–MS, 1H NMR, 13C NMR, and IR. NMR and IR spectra were consistent with the structure of α-T3E. 1H NMR (CDCl3) spectrum: 1.27 (3H, s), 1.59 (9H, s), 1.67 (3H, s), 2.00 (3H, s), 2.09 (3H, s), 2.12 (3H, s), 1.70–2.15 (16H, m), 2.57 (2H, t, J = 7.8 Hz), 2.65 (2H, t, J = 6.5 Hz), 3.68 (2H, t, J = 7.7 Hz), 4.95–5.25 (3H, m), 8.5 (1H, broad). IR (KBr) spectrum: 3200–3400 cm−<sup>1</sup> (carboxylic OH) and 1710 cm−<sup>1</sup> (C6=O).

#### *4.3. Cell Culture*

H2452, PANC1, and A549 cells were purchased for ATCC (Manassas, VA, USA). PANC1 and A549 were routinely grown in RPMI1640 supplemented with 10% FBS, 50 IU/mL penicillin, and 50 μg/mL streptomycin, and H2452 cells were routinely grown in RPMI1640 supplemented with 10% FBS, 6.5 mg/mL glucose, 1 mM sodium pyruvate, 10 mM HEPES, 50 IU/mL penicillin, and 50 μg/mL streptomycin at 37 ◦C in a humidified atmosphere with 5% CO2. Exponentially growing cells were used in experiments. Cells were plated on culture plates and cultured for 24 h to permit adherence. Cells were then cultured in RPMI1640 supplemented with 2% FBS for the indicated period, and each parameter was then examined.
