**5. Conclusions**

Our findings demonstrate that EEOS disturbed the proliferation, angiogenesis, and migration of A549 cells, which may result from the disruption of cell adhesion and migration, as shown by the CCK-8 assay and scratch wound healing assay, as a consequence of the downregulation of αvβ3 integrins, α5β1 integrins, and VEGF. As a result, EEOS may represent a good therapeutic candidate for the treatment of lung adenocarcinoma. Further studies using in vivo methods are required to fully validate our findings in human lung adenocarcinoma growth.

**Author Contributions:** Conceptualization, H.W. and D.L.K.; Methodology, H.W. and D.L.K.; Software, U.K.; Validation, H.W. and U.K.; formal analysis, U.K., H.W. and S.K.; Investigation, U.K., H.W. and D.A.A.N.; Resources, H.W., D.L.K. and D.A.A.N.; Data Curation, U.K., D.L.K. and H.W.; Writing—Original Draft Preparation, H.W. and U.K.; Writing—Review and Editing, H.W., S.E., S.K., D.A.A.N. and D.L.K.; Visualization, H.W.; Supervision, H.W. and D.L.K. Project Administration, H.W. and D.L.K.; Funding Acquisition, H.W. All authors have read and agreed to the published version of the manuscript.

**Funding:** The research was funded by the Ministry of Education, Culture, Research and Technology for the PDUPT Grant Universitas Gadjah Mada, with the grant number 1703/UN1/DITLIT/DIT-LIT/PT.01.02/2022.

**Institutional Review Board Statement:** The experimental procedures were approved by the Ethical Committee of the Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia (approval number 00057/EC-FKH/Int./2021).

**Informed Consent Statement:** Not applicable.

**Data Availability Statement:** Not applicable.

**Acknowledgments:** The authors would like to thank the Ministry of Education, Culture, Research and Technology for the research funding (Basic Research) with the grant number 1703/UN1/DITLIT/DIT-LIT/PT.01.02/2022. The authors also thank the Pharmacology Laboratory, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, and Mosa Rini Nurul H. for their excellent technical assistance.

**Conflicts of Interest:** The authors declare no conflict of interest.
