*2.1. Preparation of Ocimum sanctum Linn. Ethanolic Extract (EEOS)*

The leaves of *Ocimum sanctum* Linn. simplisia were obtained from CV. Merapi Herbal, Yogyakarta, Indonesia, and the species was identified at the Department of Biology, Gadjah Mada University (Yogyakarta, Indonesia). The ethanolic extract was obtained by a maceration technique. A total of 4000 mL of 96% ethanol (Merck, Darmstadt, Germany) was added to 300 g simplicia *Ocimum sanctum* Linn. The filtration results were concentrated using a vacuum rotary evaporator (Heidolph, Schwabach, Germany), and 8.82% *w*/*w* of *Ocimum sanctum* Linn. ethanol extract was obtained in the form of a paste.
