*4.2. PPI Network Analysis and Identification of Hub Genes*

The STRING database [58] was applied to build the PPI network. The confidence score for protein–protein interactions was selected as >0.9. The PPI network for genistein against human cervical carcinoma was visualized using Cytoscape (v3.7.1). The hub genes in the PPI network were calculated using the CytoHubba plugin based on the MCC algorithm [59].

#### *4.3. GO and KEGG Pathway Enrichment Analyses of Core Targets*

The Database for Annotation, Visualization, and Integrated Discovery (DAVID) (https://david.ncifcrf.gov/) (accessed on 17 September 2018) was used for Gene Ontology and KEGG pathway enrichment analysis of core targets. Bubble charts of BP and KEGG pathways were produced by ggplot2, implemented in the R platform.

#### *4.4. Experimental Verification*

#### 4.4.1. Reagent Source

Fetal bovine serum (FBS), 100× penicillin, streptomycin, and Dulbecco's modified Eagle's medium (DMEM) were purchased from Lonza (Verviers, Belgium). Genistein was obtained from Selleck (Shanghai, China). Genistein was dissolved in DMSO with a stock solution of 100 mM and diluted in culture medium to final concentrations (12.5–100 μM). Phosphatase and protease inhibitor cocktail set I and WST-8 buffer were purchased from Biotool (Shanghai, China). Enhanced chemiluminescence (ECL) detection buffer was purchased from Beyotime Biotechnology (Shanghai, China). Antibodies used in this study were purchased from Cell Signaling Technology (Danvers, MA, USA), and are listed in Table 1.

**Table 1.** List of antibodies used in the experiments.

