*4.6. Colony Formation Assay*

The cells were plated into 6-well culture plates at a density of 1 × 103 cells/well. After 24 h, cells were cultured for an additional 10 d in the absence (control) or presence of JI017 (25, 50, 100, and 150 μg/mL) to allow colony formation. Colonies were stained with a 1% crystal violet solution (Amersco, Solon, OH, USA).
