*3.4. Cell Viability Assays*

CRC cells (3–5 × 103 cells/well) were seeded in 96-well plates and incubated overnight. Then, the cells were treated with PSY for 72 h. After the treatment, cell viability was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assays as previously described [40]. After dissolving formazan crystals with DMSO, the absorbance was monitored at 570 nm using a microplate reader (Molecular Devices, CA, USA Data are presented as standard error of means (SEM) from at least three independent experiments in triplicate.
