*3.10. Ultra-Performance Liquid Chromatography Quadrupole Time of Flight Mass Spectrophotometry (UPLC-ESI-QTOF MS/MS) Analysis*

Chromatographic analysis of the extract was performed to identify and assess the quality of the chemical components of the EtOH-Ex and Water-Ex. The LC-MS/MS system consisted of a Thermo Scientific Vanquish UHPLC system (Sunnyvale, CA, USA) with an ACQUITY UPLC HSS T3 column (2.1 mm × 100 mm, 1.8 <sup>μ</sup>m; WatersTM,, Milford, MA, USA) and a Triple TOF 5600+ MS system (QTOF MS/MS, SCIEX, Foster City, CA, USA). The QTOF MS was equipped with an ESI source and used to complete the high-resolution experiment.
