*Article* **Ethanolic Extract of** *Ocimum sanctum* **Linn. Inhibits Cell Migration of Human Lung Adenocarcinoma Cells (A549) by Downregulation of Integrin** α**v**β**3,** α**5**β**1, and VEGF**

**Ulayatul Kustiati 1, Suleyman Ergün 2, Srikanth Karnati 2, Dwi Aris Agung Nugrahaningsih 3, Dwi Liliek Kusindarta <sup>1</sup> and Hevi Wihadmadyatami 1,\***


**Abstract:** Adenocarcinoma lung cancer is a type of non-small cell lung carcinoma (NSCLC), which accounts for 85% of lung cancer incidence globally. The therapies that are being applied, both conventional therapies and antibody-based treatments, are still found to have side effects. Several previous studies have demonstrated the ability of the ethanolic extract of *Ocimum sanctum* Linn. (EEOS) as an ethnomedicine with anti-tumor properties. The aim of this study was to determine the effect of *Ocimum sanctum* Linn. ethanolic extract in inhibiting the proliferation, angiogenesis, and migration of A549 cells (NSCLC). The adhesion as well as the migration assay was performed. Furthermore, enzyme-linked immunosorbent assay (ELISA) was used to measure the expression of αvβ3 integrins, α5β1 integrins, and VEGF. The cells were divided into the following treatment groups: control (non-treated/NT), positive control (AP3/inhibitor β3 80 μg/mL), cisplatin (9 μg/mL), and EEOS at concentrations of 50, 70, 100, and 200 μg/mL. The results showed that EEOS inhibits the adhesion ability and migration of A549 cells, with an optimal concentration of 200 μg/mL. ELISA testing showed that the group of A549 cells given EEOS 200 μg/mL presented a decrease in the optimal expression of integrin α5β1, integrin αvβ3, and VEGF.

**Keywords:** EEOS; A549 cell line; integrin α5β1; integrin αvβ3; VEGF
