Recent Insights of the Role of Microorganisms in Bovine Medicine

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Veterinary Microbiology".

Deadline for manuscript submissions: 31 March 2026 | Viewed by 2718

Special Issue Editor


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Royal GD Animal Health, Arnsbergstraat 7, 7400 AA Deventer, The Netherlands
Interests: animal health monitoring; claw health control; mycoplasma management; parasitic diseases; zoonosis
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Special Issue Information

Dear Colleagues,

Livestock, including cattle, are perpetually exposed to a myriad of microorganisms (MOs) from their surroundings. These microorganisms can either be ubiquitous environmental MOs or specific to conspecifics within the herd (Robinson et al., 2018). Animals typically show resistance to most MOs through specific or age-related immunity, yet a minority of MOs can lead to pathogenic infections (Smith and Jones, 2020).

Diagnosis and Pathogenesis: The effective management of pathogenic infections hinges on reliable diagnostic methods. These methods are informed by clinical observations from field practitioners and laboratory tests such as ELISA, PCR, and microbial cultivation (Brown et al., 2021). These tests, noted for their sensitivity and specificity, are often complemented by pathological findings during necropsy, providing deeper insights into the pathogenesis of specific diseases (Mehrvar et al., 2021).

Epidemiology and Control: Laboratory data are instrumental in understanding the epidemiology of infections and monitoring the prevalence of different MOs at the herd or sector level. This epidemiological insight is vital for devising appropriate treatment and prevention strategies (Wilson, 2017). For instance, the identification of antibiotic resistance patterns and the development of vaccines are critical components of disease control (Godfroid et al., 2013).

The objective of this Special Issue, facilitated by MDPI, is to consolidate recent insights from various domains—clinical observations, laboratory tests, scientific research, and industrial developments—regarding MO infections in cattle, veal calves, and beef cattle farms. By integrating this information, we aim to enhance the welfare of animals and their caretakers and improve disease control measures for infections caused by MOs.

This compilation of recent findings will contribute to generating a better understanding of the role played by microorganisms in bovine medicine. By leveraging clinical and laboratory data, scientific research, and industry advancements, we are able to develop more effective strategies for the treatment and prevention of MO infections in cattle. This holistic approach is essential for improving animal welfare and managing diseases more effectively.

Dr. Menno Holzhauer

Royal GD Animal Health

e-mail: m.holzhauer@gdanimalhealth.com

phone: 0031-651612496

References:

  • Brown, E., Nelson, N., Gubbins S, Colenutt, C. (2021). Environmental and air sampling are efficient methods for the detection and quantification of foot-and-mouth disease virus. of Virol. Meth. 287, 113988
  • Godfroid J., Al Dahouk S., Pappas G., Roth F., Matope G., Muma J., Marcotty T., Pfeiffer D. and Skjerve A.E. (2013) A “One Health” surveillance and control of brucellosis in developing countries: moving away from improvisation. Comp Immunol Microbiol Infec Dis. 36, 241‑248.
  • Mehrvar S, Himmel LE, Babburi P, Goldberg AL, Guffroy M, Janardhan K, et al. (2021) Deep learning approaches and applications in toxicologic histopathology: Current status and future perspectives. J Pathol Inform 2021;12:42.
  • Robinson, T.P., et al. (2018). "Global livestock production systems." Food and Agric. Organiz. of the United Nations.
  • Smith, R.A., and Jones, D.E. (2020). "Economic impact of livestock diseases." Journal, 202, 151-159.
  • Wilson, D., Jackson, T., Sapey, E. and Lord, J.M. (2017) Frailty and sarcopenia: the potential role of an aged immune system. Ageing Res. Rev.36, 1–10.

Dr. Menno Holzhauer
Guest Editor

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Keywords

  • Virology
  • bacteriology
  • parasitology
  • aetiology
  • diagnostic
  • laboratory testing
  • treatment
  • prevention

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Published Papers (3 papers)

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Research

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13 pages, 2282 KB  
Article
Interaction Between Duddingtonia flagrans and Pochonia chlamydosporia for the Biological Control of Bovine Gastrointestinal Nematodes
by Maria Larissa Bitencourt Vidal, Júlia dos Santos Fonseca, Ítalo Stoupa Vieira, Lorena Souza Castro Altoé, Lorendane Millena de Carvalho, Wagner Nunes Rodrigues, Isabella Vilhena Freire Martins and Jackson Victor de Araújo
Microorganisms 2026, 14(1), 85; https://doi.org/10.3390/microorganisms14010085 - 30 Dec 2025
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Abstract
Gastrointestinal nematodes are among the most significant parasites affecting livestock health and productivity, leading to major economic losses and contributing to the global increase in resistance to anthelmintics. Biological control using fungi with ovicidal and nematophagous activity offers an environmentally friendly alternative. This [...] Read more.
Gastrointestinal nematodes are among the most significant parasites affecting livestock health and productivity, leading to major economic losses and contributing to the global increase in resistance to anthelmintics. Biological control using fungi with ovicidal and nematophagous activity offers an environmentally friendly alternative. This trial represents the first long-term field evaluation in cattle of the commercial combination of D. flagrans and P. chlamydosporia under natural infection conditions. Eighteen Holstein × Zebu males (12–15 months old) were divided into three groups (n = 6): T1 (D. flagrans), T2 (D. flagrans + P. chlamydosporia), and control. Treatments were administered orally daily (6 g/100 kg BW of each fungus; 106 chlamydospores/g) for nine months. Fecal egg counts (EPG) and infective larvae in pasture (L3) were monitored. Groups T1 and T2 showed significantly lower EPG values than the control during most of the experimental period. Haemonchus spp. was identified as the predominant nematode, supporting its epidemiological relevance. The combined fungal treatment exhibited enhanced effectiveness, enhancing parasite suppression through complementary ovicidal and larvicidal mechanisms. This approach offers a sustainable alternative to the excessive use of chemical compounds and has the potential to contribute to integrated animal health and livestock production. Full article
(This article belongs to the Special Issue Recent Insights of the Role of Microorganisms in Bovine Medicine)
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29 pages, 3984 KB  
Article
Comparative In Vitro Killing of Key Bovine Respiratory Bacterial Pathogens by Pradofloxacin and Eight Other Veterinary Antimicrobial Agents
by Joseph M. Blondeau and Shantelle D. Fitch
Microorganisms 2025, 13(12), 2681; https://doi.org/10.3390/microorganisms13122681 - 25 Nov 2025
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Abstract
Pradofloxacin is a third-generation dual enzyme targeting bactericidal veterinary fluoroquinolone, recently approved for use in cattle for bovine respiratory disease, which is active against Gram-positive/negative, atypical and anaerobic bacteria. We compared in vitro killing by pradofloxacin to that by ceftiofur, danofloxacin, enrofloxacin, florfenicol, [...] Read more.
Pradofloxacin is a third-generation dual enzyme targeting bactericidal veterinary fluoroquinolone, recently approved for use in cattle for bovine respiratory disease, which is active against Gram-positive/negative, atypical and anaerobic bacteria. We compared in vitro killing by pradofloxacin to that by ceftiofur, danofloxacin, enrofloxacin, florfenicol, marbofloxacin, tildipirosin, tilmicosin and tulathromycin against bovine isolates of Mannheimia haemolytica and Pasteurella multocida over a range of bacterial densities (106–109 cfu/mL). Drug concentrations used in the kill assays included the minimum inhibitory and mutant prevention drug concentrations and maximum serum and maximum tissue drug concentrations. Regardless of bacteria density tested and drug concentration used, pradofloxacin consistently killed as many or more (but not fewer) bacterial cells than any other drug tested against M. haemolytica strains. At the 108–109 cfu/mL densities, pradofloxacin killed 99–99.9%, 100% and 100% of bacterial cells at the MPC, maximum serum and maximum tissue drug concentrations, respectively, following 24 h of drug exposure. Indeed, pradofloxacin killed 99.9–99.99% of cells following 30–60 min of exposure to the maximum serum concentration. Similar trends were seen with killing of P. multocida strains by pradofloxacin. Against high-density bacterial populations, pradofloxacin was rapidly bactericidal and consistently killed more cells than the other agents tested. This manuscript represents the most comprehensive comparative in vitro kill study completed to date. Full article
(This article belongs to the Special Issue Recent Insights of the Role of Microorganisms in Bovine Medicine)
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Review

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19 pages, 1064 KB  
Review
Review of White Line Disorders in Zone 3 and Toe Tip Necrosis in Dairy Cows and Recent Insights into Aetiopathogenesis and Treatments
by Menno Holzhauer and Han de Leeuw
Microorganisms 2025, 13(9), 2159; https://doi.org/10.3390/microorganisms13092159 - 16 Sep 2025
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Abstract
White line disorders represent the most prevalent claw horn disruption lesion in dairy cattle. Recent studies have yielded new insights into the appropriate treatment modalities for these lesions. The aims of this study are to elucidate the pathogenesis of white line disorders and [...] Read more.
White line disorders represent the most prevalent claw horn disruption lesion in dairy cattle. Recent studies have yielded new insights into the appropriate treatment modalities for these lesions. The aims of this study are to elucidate the pathogenesis of white line disorders and its associated claw lesions, such as toe tip necrosis, and to discuss practical treatment applications. In Western Europe, many herds are endemically infected with digital dermatitis. White line disorders in zone 3 and toe tip necrosis starting in zone 1—often beginning as axial white line lesions—frequently exhibit a suboptimal response to standard treatments, including corrective trimming, the application of a hoof block on the healthy claw and the administration of NSAIDs, due to secondary infections with Treponema spp. This study addresses the current perspectives on the aetiopathogenesis of white line disorders and the therapeutic challenges in promoting complete recovery and the correct use of antibiotics, along with preventive measures, such as good flooring. An important factor of its pathogenesis is a decrease in body condition around parturition, Correct diagnosis can be made via the use of regular locomotion scoring and good diagnostic tools, and thin soles by among others overtrimming should be prevented. Current therapeutic methods consist of the prompt application of a block and an NSAID and, in some circumstances, a parenteral injection with antibiotics when there is no good response to the applied therapies. Full article
(This article belongs to the Special Issue Recent Insights of the Role of Microorganisms in Bovine Medicine)
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