Epidemiology, Pathology and Diagnostic Methods of Fruit Tree and Grapevine Viruses

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Protection and Biotic Interactions".

Deadline for manuscript submissions: closed (20 March 2023) | Viewed by 2530

Special Issue Editors


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Guest Editor
Department of Virology, Plant Protection and Biotechnology Center, Instituto Valenciano de Investigaciones Agrarias (IVIA), 46113 Moncada, Valencia, Spain
Interests: plant virology; molecular diagnosis; NGS applied to virus diagnostics; development and validation of new techniques for virus detection and identification
Special Issues, Collections and Topics in MDPI journals

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Guest Editor
Department of Virology, Plant Protection and Biotechnology Center, Instituto Valenciano de Investigaciones Agrarias, 46113 Moncada, Valencia, Spain
Interests: HTS; plant virology; diagnostics; molecular characterization of plant viruses
Special Issues, Collections and Topics in MDPI journals

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Guest Editor
Laboratory of Plant Pathology, School of Agriculture, Faculty of Agriculture, Forestry and Natural Environment, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece
Interests: HTS; plant virus diagnostics; molecular characterization of plant viruses; plant virus epidemiology

Special Issue Information

Dear Colleagues,

Viruses are an important limiting factor in the production of plant-based foods and their processed products, by directly affecting crop yield and quality. Perennial hosts, such as fruit trees and grapevine, tend to accumulate multiple virus species/strains/isolates either via the propagation of infected material or through additional infections during their longer lifespans. Infection by a pathogenic virus/virus isolate manifests into a disease which, in addition to the reduction in yield and lower quality of products, can also shorten the productive life of the orchard/vineyard or kill the host, resulting in loss of capital.

Over the past couple of decades, the number of plant viruses has been growing at a fast pace, mainly due to the advent of molecular techniques and high-throughput sequencing. However, knowledge regarding the biological characteristics of these viruses has not been able to keep up, resulting in the accumulation of viral sequences in databases which lack a biological context. Moreover, the discovery of new genetic variants of already characterized viruses directly affects the robustness and specificity of the diagnostic methods in use. It is imperative that we gain insight into the pathology and epidemiology of these new isolates so they can be evaluated as a biological entity rather than a pool of sequence data. This knowledge will lead to implementing control programs according to the impact these viruses have, in contrast to considering all viral isolates/strains and species as high-concern ones.

This Special Issue aims to advance the knowledge regarding plant virus pathology and epidemiology, presenting information about the accumulation, symptoms, spread, impact, vectors, and host range of new and known viruses, as well as presenting novel or re-evaluating already established diagnostic methods for viruses infecting fruit trees and grapevine.

Dr. Antonio Olmos
Dr. Ana Belen Ruiz-Garcia
Dr. Leonidas Lotos
Guest Editors

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Keywords

  • diagnostic methods
  • virus pathology
  • virus epidemiology
  • HTS
  • Fruit Tree Viruses
  • Grapevine Viruses

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Published Papers (1 paper)

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Research

15 pages, 2704 KiB  
Article
Incidence and Genetic Diversity of Grapevine Virus G in Croatian Vineyards
by Martin Jagunić, Alfredo Diaz-Lara, Lóránt Szőke, Maher Al Rwahnih, Kristian Stevens, Goran Zdunić and Darko Vončina
Plants 2022, 11(18), 2341; https://doi.org/10.3390/plants11182341 - 7 Sep 2022
Cited by 1 | Viewed by 2181
Abstract
Grapevine virus G (GVG) is a recently discovered vitivirus infecting grapevines. Historically, viruses in the genus Vitivirus have been associated with the grapevine rugose wood disease. Based on new and previously reported GVG isolates, primers and probes were developed for real-time RT-PCR. The [...] Read more.
Grapevine virus G (GVG) is a recently discovered vitivirus infecting grapevines. Historically, viruses in the genus Vitivirus have been associated with the grapevine rugose wood disease. Based on new and previously reported GVG isolates, primers and probes were developed for real-time RT-PCR. The developed assay successfully detected the virus in infected plants during dormancy and the growing season. A field study of 4327 grapevines from Croatian continental and coastal wine-growing regions confirmed the presence of GVG in 456 (~10.5%) grapevines from three collection plantations and 77 commercial vineyards, with infection rates ranging from 2% to 100%. Interestingly, the virus was confirmed only in vines considered to be Croatian autochthonous cultivars, but not in introduced cultivars. A 564-nucleotide long portion of the coat protein gene from previously known and newly characterized GVG isolates had nucleotide and amino acid identities ranging from 89% to 100% and from 96.8% to 100%, respectively. Phylogenetic analysis revealed five distinct groups, with isolates originating from the same site being close to each other, indicating possible local infection. The information presented in this manuscript sets the stage for future studies to better understand the ecology and epidemiology of GVG and the possible need for inclusion in certification schemes. Full article
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