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Optical Sensing Methods for Microorganism Identification

A special issue of Sensors (ISSN 1424-8220). This special issue belongs to the section "Chemical Sensors".

Deadline for manuscript submissions: closed (15 September 2023) | Viewed by 6952

Special Issue Editor


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Guest Editor
Interdisciplinary Institute for Technological Innovation (3IT), CNRS IRL-3463, Université de Sherbrooke, 3000, Boulevard de l’Université, Sherbrooke, QC J1K 0A5, Canada
Interests: laser ablation/processing; optical spectroscopy; bacteria identification; FTIR/Raman; surface characterization

Special Issue Information

Dear Colleagues,

Microorganisms include bacteria, fungi, and viruses. Pathogenic microorganisms are causative agents of various infectious diseases that are beginning to pose an increasingly serious threat worldwide. Pathogenic microorganisms can also undergo mutations and develop resistance to antimicrobial agents, which complicates diagnostic and therapeutic regimens. Pathogenic bacteria detection using biosensors remains a rich subject to be explored by researchers, and many tools have been reported using different transducers. Optical biosensors allow for rapid, accurate, portable and cost-effective diagnosis for the identification of microorganisms. Among these biosensors, the development of an innovative technology combining lasers and spectroscopy to improve the sensitivity and rapidness of microbial identification results is of current interest to researchers. The use of lasers for ablating biological materials will serve to transfer materials without ionization from the test substrate to a carrier substrate, and will help in the investigation of the effects of laser-induced decomposition on the potential efficiency of fingerprinting. Applying the proposed technique to laboratory strains of bacteria will pave the way toward investigating complex environments for pathogenic microorganisms.

This Special Issue encourages authors to submit their research and contributions on the use and application of optical biosensors and sensors for the detection of analytes in microorganisms.

Dr. Khalid Moumanis
Guest Editor

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Keywords

  • laser ablation/processing
  • optical spectroscopy
  • bacteria identification
  • FTIR/Raman
  • surface characterization

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Published Papers (3 papers)

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Research

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14 pages, 3795 KiB  
Article
Quick Detection of Proteus and Pseudomonas in Patients’ Urine and Assessing Their Antibiotic Susceptibility Using Infrared Spectroscopy and Machine Learning
by George Abu-Aqil, Itshak Lapidot, Ahmad Salman and Mahmoud Huleihel
Sensors 2023, 23(19), 8132; https://doi.org/10.3390/s23198132 - 28 Sep 2023
Cited by 1 | Viewed by 1482
Abstract
Bacterial resistance to antibiotics is a primary global healthcare concern as it hampers the effectiveness of commonly used antibiotics used to treat infectious diseases. The development of bacterial resistance continues to escalate over time. Rapid identification of the infecting bacterium and determination of [...] Read more.
Bacterial resistance to antibiotics is a primary global healthcare concern as it hampers the effectiveness of commonly used antibiotics used to treat infectious diseases. The development of bacterial resistance continues to escalate over time. Rapid identification of the infecting bacterium and determination of its antibiotic susceptibility are crucial for optimal treatment and can save lives in many cases. Classical methods for determining bacterial susceptibility take at least 48 h, leading physicians to resort to empirical antibiotic treatment based on their experience. This random and excessive use of antibiotics is one of the most significant drivers of the development of multidrug-resistant (MDR) bacteria, posing a severe threat to global healthcare. To address these challenges, considerable efforts are underway to reduce the testing time of taxonomic classification of the infecting bacterium at the species level and its antibiotic susceptibility determination. Infrared spectroscopy is considered a rapid and reliable method for detecting minor molecular changes in cells. Thus, the main goal of this study was the use of infrared spectroscopy to shorten the identification and the susceptibility testing time of Proteus mirabilis and Pseudomonas aeruginosa from 48 h to approximately 40 min, directly from patients’ urine samples. It was possible to identify the Proteus mirabilis and Pseudomonas aeruginosa species with 99% accuracy and, simultaneously, to determine their susceptibility to different antibiotics with an accuracy exceeding 80%. Full article
(This article belongs to the Special Issue Optical Sensing Methods for Microorganism Identification)
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Review

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30 pages, 6696 KiB  
Review
Optical Sensors for Bacterial Detection
by Olga I. Guliy, Olga A. Karavaeva, Andrey V. Smirnov, Sergei A. Eremin and Viktor D. Bunin
Sensors 2023, 23(23), 9391; https://doi.org/10.3390/s23239391 - 24 Nov 2023
Cited by 3 | Viewed by 2369
Abstract
Analytical devices for bacterial detection are an integral part of modern laboratory medicine, as they permit the early diagnosis of diseases and their timely treatment. Therefore, special attention is directed to the development of and improvements in monitoring and diagnostic methods, including biosensor-based [...] Read more.
Analytical devices for bacterial detection are an integral part of modern laboratory medicine, as they permit the early diagnosis of diseases and their timely treatment. Therefore, special attention is directed to the development of and improvements in monitoring and diagnostic methods, including biosensor-based ones. A promising direction in the development of bacterial detection methods is optical sensor systems based on colorimetric and fluorescence techniques, the surface plasmon resonance, and the measurement of orientational effects. This review shows the detecting capabilities of these systems and the promise of electro-optical analysis for bacterial detection. It also discusses the advantages and disadvantages of optical sensor systems and the prospects for their further improvement. Full article
(This article belongs to the Special Issue Optical Sensing Methods for Microorganism Identification)
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26 pages, 1275 KiB  
Review
Oxygen Sensor-Based Respirometry and the Landscape of Microbial Testing Methods as Applicable to Food and Beverage Matrices
by Dmitri B. Papkovsky and Joseph P. Kerry
Sensors 2023, 23(9), 4519; https://doi.org/10.3390/s23094519 - 6 May 2023
Cited by 8 | Viewed by 2701
Abstract
The current status of microbiological testing methods for the determination of viable bacteria in complex sample matrices, such as food samples, is the focus of this review. Established methods for the enumeration of microorganisms, particularly, the ‘gold standard’ agar plating method for the [...] Read more.
The current status of microbiological testing methods for the determination of viable bacteria in complex sample matrices, such as food samples, is the focus of this review. Established methods for the enumeration of microorganisms, particularly, the ‘gold standard’ agar plating method for the determination of total aerobic viable counts (TVC), bioluminescent detection of total ATP, selective molecular methods (immunoassays, DNA/RNA amplification, sequencing) and instrumental methods (flow cytometry, Raman spectroscopy, mass spectrometry, calorimetry), are analyzed and compared with emerging oxygen sensor-based respirometry techniques. The basic principles of optical O2 sensing and respirometry and the primary materials, detection modes and assay formats employed are described. The existing platforms for bacterial cell respirometry are then described, and examples of particular assays are provided, including the use of rapid TVC tests of food samples and swabs, the toxicological screening and profiling of cells and antimicrobial sterility testing. Overall, O2 sensor-based respirometry and TVC assays have high application potential in the food industry and related areas. They detect viable bacteria via their growth and respiration; the assay is fast (time to result is 2–8 h and dependent on TVC load), operates with complex samples (crude homogenates of food samples) in a simple mix-and-measure format, has low set-up and instrumentation costs and is inexpensive and portable. Full article
(This article belongs to the Special Issue Optical Sensing Methods for Microorganism Identification)
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