Differential Metabolic and Transcriptional Responses of Gilthead Seabream (Sparus aurata) Administered with Cortisol or Cortisol-BSA
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Experimental Design
2.2. Measurement of Plasma Cortisol and Tissue Metabolites
2.3. RNA Extraction and cDNA Synthesis
2.4. Real Time-PCR
2.5. Statistical Analysis
3. Results
3.1. Plasma Indicators in S. aurata Administered with Cortisol or Cortisol-BSA
3.2. Energetic Metabolites in Liver and Skeletal Muscle of S. aurata Administered with Cortisol or Cortisol-BSA
3.3. Corticosteroid Receptor-Related Genes Expression in Liver and Skeletal Muscle of S. aurata Administered with Cortisol or Cortisol-BSA
3.4. Glucose Metabolism—And Atrophy-Related Genes in S. aurata Administered with Cortisol or Cortisol-BSA
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Parameter | SHAM | CORT | BSA | CORT-BSA |
---|---|---|---|---|
Glucose (mmol/L) | 4.07 ± 0.19 a | 6.95 ± 0.39 b | 4.07 ± 0.25 a | 3.76 ± 0.16 a |
Triacylglycerols (mg/dL) | 92.64 ± 14.12 a | 257.39 ± 24.64 b | 66.69 ± 2.76 a | 64.52 ± 6.69 a |
Lactate (mmol/L) | 2.11 ± 0.28 | 2.58 ± 0.24 | 1.60 ± 0.26 | 1.66 ± 0.23 |
Protein (mg/mL) | 31.76 ± 1.27 | 34.76 ± 1.16 | 28.64 ± 2.11 | 30.39 ± 0.91 |
Parameter | SHAM | CORT | BSA | CORT-BSA |
---|---|---|---|---|
Glucose (mg/g) | 1.05 ± 0.17 | 1.16 ± 0.16 | 1.05 ± 0.03 | 1.16 ± 0.07 |
Triacylglycerols (mg/g) | 0.14 ± 0.02 | 0.27 ± 0.08 | 0.12 ± 0.02 | 0.12 ± 0.01 |
Lactate (mg/g) | 0.011 ± 0.005 | 0.014 ± 0.004 | 0.013 ± 0.006 | 0.009 ± 0.002 |
Glycogen (mg/g) | 0.27 ± 0.11 a | 0.79 ± 0.09 b | 0.20 ± 0.07 a | 0.23 ± 0.06 a |
Parameter | SHAM | CORT | BSA | CORT-BSA |
---|---|---|---|---|
Glucose (mg/g) | 2.16 ± 0.14 | 2.18 ± 0.08 | 1.94 ± 0.03 | 2.05 ± 0.09 |
Triacylglycerols (mg/g) | 1.29 ± 0.54 | 2.94 ± 1.17 | 0.45 ± 0.15 | 1.17 ± 0.34 |
Lactate (mg/g) | 0.29 ± 0.02 | 0.34 ± 0.03 | 0.24 ± 0.02 | 0.27 ± 0.02 |
Glycogen (mg/g) | 0.66 ± 0.08 | 0.97 ± 0.12 | 0.33 ± 0.06 | 0.79 ± 0.15 |
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Aedo, J.; Aravena-Canales, D.; Ruiz-Jarabo, I.; Oyarzún, R.; Molina, A.; Martínez-Rodríguez, G.; Valdés, J.A.; Mancera, J.M. Differential Metabolic and Transcriptional Responses of Gilthead Seabream (Sparus aurata) Administered with Cortisol or Cortisol-BSA. Animals 2021, 11, 3310. https://doi.org/10.3390/ani11113310
Aedo J, Aravena-Canales D, Ruiz-Jarabo I, Oyarzún R, Molina A, Martínez-Rodríguez G, Valdés JA, Mancera JM. Differential Metabolic and Transcriptional Responses of Gilthead Seabream (Sparus aurata) Administered with Cortisol or Cortisol-BSA. Animals. 2021; 11(11):3310. https://doi.org/10.3390/ani11113310
Chicago/Turabian StyleAedo, Jorge, Daniela Aravena-Canales, Ignacio Ruiz-Jarabo, Ricardo Oyarzún, Alfredo Molina, Gonzalo Martínez-Rodríguez, Juan Antonio Valdés, and Juan Miguel Mancera. 2021. "Differential Metabolic and Transcriptional Responses of Gilthead Seabream (Sparus aurata) Administered with Cortisol or Cortisol-BSA" Animals 11, no. 11: 3310. https://doi.org/10.3390/ani11113310