Elevation of Cleaved p18 Bax Levels Associated with the Kinetics of Neuronal Cell Death during Japanese Encephalitis Virus Infection
Abstract
:1. Introduction
2. Results
2.1. JEV Infection of SH-SY5Y Cells
2.2. JEV Infection Induces Cell Death in SH-SY5Y Cells
2.3. JEV Infection Induces Apoptosis in SH-SY5Y Cells
2.4. Expression of Bcl-2 Family Members in JEV-Infected SH-SY5Y Cells
2.5. JEV Infection Increases p18 Bax Accumulation in Mitochondria and Induced Cyt c Release into the Cytosol
2.6. JEV Infection Triggers Apoptosis by Inducing Caspase-3/7 Activity
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. JEV Propagation in Vero Cells
4.3. Virus Titration Assay
4.4. JEV Infection in Neuronal SH-SY5Y Cells
4.5. Cell Viability Assay
4.6. Western Blot Analysis
4.7. Apoptotic Cell Death Analysis
4.8. Cytochrom c Releasing Assay
4.9. Caspase Activity Assay
4.10. Statistical Analysis
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Wongchitrat, P.; Samutpong, A.; Lerdsamran, H.; Prasertsopon, J.; Yasawong, M.; Govitrapong, P.; Puthavathana, P.; Kitidee, K. Elevation of Cleaved p18 Bax Levels Associated with the Kinetics of Neuronal Cell Death during Japanese Encephalitis Virus Infection. Int. J. Mol. Sci. 2019, 20, 5016. https://doi.org/10.3390/ijms20205016
Wongchitrat P, Samutpong A, Lerdsamran H, Prasertsopon J, Yasawong M, Govitrapong P, Puthavathana P, Kitidee K. Elevation of Cleaved p18 Bax Levels Associated with the Kinetics of Neuronal Cell Death during Japanese Encephalitis Virus Infection. International Journal of Molecular Sciences. 2019; 20(20):5016. https://doi.org/10.3390/ijms20205016
Chicago/Turabian StyleWongchitrat, Prapimpun, Arisara Samutpong, Hatairat Lerdsamran, Jarunee Prasertsopon, Montri Yasawong, Piyarat Govitrapong, Pilaipan Puthavathana, and Kuntida Kitidee. 2019. "Elevation of Cleaved p18 Bax Levels Associated with the Kinetics of Neuronal Cell Death during Japanese Encephalitis Virus Infection" International Journal of Molecular Sciences 20, no. 20: 5016. https://doi.org/10.3390/ijms20205016