Adult Exposure to Di-N-Butyl Phthalate (DBP) Induces Persistent Effects on Testicular Cell Markers and Testosterone Biosynthesis in Mice
Abstract
:1. Introduction
2. Results
2.1. General Status
2.2. Steroid Hormone Concentrations in Testis and Adrenal Glands
2.3. Steroidogenic Protein Levels
2.4. Testis Histology and Cell Marker Levels
3. Discussion
4. Methods and Materials
4.1. Chemicals and Reagents
4.2. Animal Housing and Exposure
4.3. Steroid Hormone Extraction
4.4. UPLC-HRMS Analysis of Steroid Hormone Levels
4.5. Immunohistochemistry
4.6. Western Blot
4.7. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Källsten, L.; Almamoun, R.; Pierozan, P.; Nylander, E.; Sdougkou, K.; Martin, J.W.; Karlsson, O. Adult Exposure to Di-N-Butyl Phthalate (DBP) Induces Persistent Effects on Testicular Cell Markers and Testosterone Biosynthesis in Mice. Int. J. Mol. Sci. 2022, 23, 8718. https://doi.org/10.3390/ijms23158718
Källsten L, Almamoun R, Pierozan P, Nylander E, Sdougkou K, Martin JW, Karlsson O. Adult Exposure to Di-N-Butyl Phthalate (DBP) Induces Persistent Effects on Testicular Cell Markers and Testosterone Biosynthesis in Mice. International Journal of Molecular Sciences. 2022; 23(15):8718. https://doi.org/10.3390/ijms23158718
Chicago/Turabian StyleKällsten, Liselott, Radwa Almamoun, Paula Pierozan, Erik Nylander, Kalliroi Sdougkou, Jonathan W. Martin, and Oskar Karlsson. 2022. "Adult Exposure to Di-N-Butyl Phthalate (DBP) Induces Persistent Effects on Testicular Cell Markers and Testosterone Biosynthesis in Mice" International Journal of Molecular Sciences 23, no. 15: 8718. https://doi.org/10.3390/ijms23158718