Melatonin Type 2 Receptor Activation Regulates Blue Light Exposure-Induced Mouse Corneal Epithelial Damage by Modulating Impaired Autophagy and Apoptosis
Abstract
:1. Introduction
2. Results
2.1. Oxidative Stress Levels in the Cornea Induced by BL Exposuree
2.2. Melatonin Secretion and MT1/2 Activation in Corneal Tissues Due to BL Exposure
2.3. Autophagy Flux Defects in the Mouse Cornea Due to BL Exposure
2.4. BAX and Cleaved Caspase 3 Expression in Corneal Tissue induced by BL Exposure
2.5. Corneal Epithelial Damage
3. Discussion
4. Materials and Methods
4.1. Designs of Mouse Model and Experiments
4.2. ELISA
4.3. Immunohistochemistry
4.4. Western Blotting
4.5. Ocular Surface Parameters
4.6. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Conflicts of Interest
References
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Jin, R.; Li, Y.; Jin, H.; Yoon, H.S.; Choi, J.S.; Kim, J.; Yoon, H.J.; Yoon, K.C. Melatonin Type 2 Receptor Activation Regulates Blue Light Exposure-Induced Mouse Corneal Epithelial Damage by Modulating Impaired Autophagy and Apoptosis. Int. J. Mol. Sci. 2022, 23, 11341. https://doi.org/10.3390/ijms231911341
Jin R, Li Y, Jin H, Yoon HS, Choi JS, Kim J, Yoon HJ, Yoon KC. Melatonin Type 2 Receptor Activation Regulates Blue Light Exposure-Induced Mouse Corneal Epithelial Damage by Modulating Impaired Autophagy and Apoptosis. International Journal of Molecular Sciences. 2022; 23(19):11341. https://doi.org/10.3390/ijms231911341
Chicago/Turabian StyleJin, Rujun, Ying Li, Hui Jin, Hee Su Yoon, Ji Suk Choi, Jonghwa Kim, Hyeon Jeong Yoon, and Kyung Chul Yoon. 2022. "Melatonin Type 2 Receptor Activation Regulates Blue Light Exposure-Induced Mouse Corneal Epithelial Damage by Modulating Impaired Autophagy and Apoptosis" International Journal of Molecular Sciences 23, no. 19: 11341. https://doi.org/10.3390/ijms231911341