A Simple and Efficient Two-Dimensional High-Speed Counter-Current Chromatography Linear Gradient and Isocratic Elution Modes for the Preparative Separation of Coumarins from Roots of Toddalia asiatica (Linn.) Lam.
Abstract
:1. Introduction
2. Results
2.1. Selection of the HSCCC Solvent System
2.2. Optimization of HSCCC Conditions
2.3. Structure Identification
3. Materials and Methods
3.1. Reagents and Materials
3.2. Apparatus
3.3. Preparation of Crude Extract
3.4. Measurement of Partition Coefficient
3.5. Preparation of Solvent System and Sample Solution
3.6. HSCCC Separation Procedure
3.7. HPLC Analysis
3.8. Structural Identification
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Sample Availability
References
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Compound No. | KD Values (n-Hex/EtOAc/MeOH/H2O, v/v) | ||||||
---|---|---|---|---|---|---|---|
5:5:1:9 | 5:5:2:8 | 5:5:3:7 | 5:5:4:6 | 5:5:4.5:5.5 | 5:5:5:5 | 5:5:6:4 | |
1 | 1.74 | 0.90 | 0.66 | 0.33 | 0.24 | <0.20 | <0.20 |
2 | 0.94 | 0.53 | 0.40 | 0.21 | <0.20 | <0.20 | <0.20 |
3 | >5.00 | 4.27 | 2.94 | 1.17 | 0.94 | 0.54 | 0.25 |
4 | >5.00 | >5.00 | 3.23 | 1.28 | 1.05 | 0.62 | 0.30 |
5 | >5.00 | >5.00 | >5.00 | 2.89 | 2.35 | 1.25 | 0.52 |
6 | >5.00 | >5.00 | >5.00 | 3.20 | 2.68 | 1.49 | 0.68 |
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Ma, W.; Ali, I.; Li, Y.; Hussain, H.; Zhao, H.; Sun, X.; Xie, L.; Cui, L.; Wang, D. A Simple and Efficient Two-Dimensional High-Speed Counter-Current Chromatography Linear Gradient and Isocratic Elution Modes for the Preparative Separation of Coumarins from Roots of Toddalia asiatica (Linn.) Lam. Molecules 2021, 26, 5986. https://doi.org/10.3390/molecules26195986
Ma W, Ali I, Li Y, Hussain H, Zhao H, Sun X, Xie L, Cui L, Wang D. A Simple and Efficient Two-Dimensional High-Speed Counter-Current Chromatography Linear Gradient and Isocratic Elution Modes for the Preparative Separation of Coumarins from Roots of Toddalia asiatica (Linn.) Lam. Molecules. 2021; 26(19):5986. https://doi.org/10.3390/molecules26195986
Chicago/Turabian StyleMa, Wenya, Iftikhar Ali, Yali Li, Hidayat Hussain, Huanzhu Zhao, Xuan Sun, Lei Xie, Li Cui, and Daijie Wang. 2021. "A Simple and Efficient Two-Dimensional High-Speed Counter-Current Chromatography Linear Gradient and Isocratic Elution Modes for the Preparative Separation of Coumarins from Roots of Toddalia asiatica (Linn.) Lam." Molecules 26, no. 19: 5986. https://doi.org/10.3390/molecules26195986