The Detection of Periodic Reemergence Events of SARS-CoV-2 Delta Strain in Communities Dominated by Omicron
Abstract
:1. Introduction
2. Materials and Methods
2.1. Wastewater Sampling and Preparation
2.2. cDNA and Library Synthesis
2.3. Next-Generation Sequencing and Data Analysis
2.4. Accession and Analysis of City of Louisville KY Public Health Data
2.5. Statistical Analysis of the Impact of Delta Reemergence on SARS-CoV-2 Associated Deaths
3. Results
3.1. The Power and Importance of Wastewater Monitoring in the Detection of Variant Emergence
3.2. Unexplained Periodic Reemergence of the Delta Strain in Wastewatersheds of Louisville KY
4. Discussion
4.1. Weekly Monitoring of Wastewater Is Sufficient and Cost-Effective
4.2. Low Vaccine Uptake Does Not Correlate with Reemergence of the SARS-CoV-2 Delta Strain, and Reemergence Correlates with Increased Negative Outcomes to Public Health
4.3. The Detection of SARS-CoV-2 Reemergence Events Underscores the Importance of Personalized Medicine in Infectious Disease
4.4. Forward Looking to the Next Pandemic
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Westcott, C.E.; Sokoloski, K.J.; Rouchka, E.C.; Chariker, J.H.; Holm, R.H.; Yeager, R.A.; Moore, J.B., IV; Elliott, E.M.; Talley, D.; Bhatnagar, A.; et al. The Detection of Periodic Reemergence Events of SARS-CoV-2 Delta Strain in Communities Dominated by Omicron. Pathogens 2022, 11, 1249. https://doi.org/10.3390/pathogens11111249
Westcott CE, Sokoloski KJ, Rouchka EC, Chariker JH, Holm RH, Yeager RA, Moore JB IV, Elliott EM, Talley D, Bhatnagar A, et al. The Detection of Periodic Reemergence Events of SARS-CoV-2 Delta Strain in Communities Dominated by Omicron. Pathogens. 2022; 11(11):1249. https://doi.org/10.3390/pathogens11111249
Chicago/Turabian StyleWestcott, Claire E., Kevin J. Sokoloski, Eric C. Rouchka, Julia H. Chariker, Rochelle H. Holm, Ray A. Yeager, Joseph B. Moore, IV, Erin M. Elliott, Daymond Talley, Aruni Bhatnagar, and et al. 2022. "The Detection of Periodic Reemergence Events of SARS-CoV-2 Delta Strain in Communities Dominated by Omicron" Pathogens 11, no. 11: 1249. https://doi.org/10.3390/pathogens11111249