Search Results (56)

Background: Skeletal muscle aging is characterized by impaired myogenic differentiation, disrupted circadian rhythms, elevated oxidative stress, and mitochondrial dysfunction. Rutin, a natural flavonoid with antioxidant properties, has been suggested to mitigate aging processes; however, its effects on circadian regulation and muscle homeostasis remain unclear. Methods: In vitro, differentiated C2C12 myotubes were treated with D-galactose (D-gal, 20 g/L) with or without rutin (20 μM). In vivo, C57BL/6 mice were supplemented with rutin (100 mg/kg b.w.) via oral gavage in a D-gal-induced aging mouse model (150 mg/kg b.w., i.p.). Results: D-gal induced cellular senescence, impaired myogenic differentiation, disrupted circadian oscillations, increased oxidative stress, and compromised mitochondrial function. Rutin treatment restored myotube formation, enhanced circadian rhythmicity of differentiation-related genes, and corrected the antiphase patterns of Per2 and Rorc. It also reduced reactive oxygen species and malondialdehyde levels; increased superoxide dismutase, catalase, and glutathione peroxidase activity; improved ATP production and membrane potential; and decreased mitochondrial oxidative aging, as confirmed by pMitoTimer imaging. Furthermore, rutin reinstated the rhythmic expression of oxidative phosphorylation proteins and Pgc1α. In vivo, rutin supplementation enhanced muscle performance (prolonged hanging time) and oxidative capacity, particularly at night (ZT14–ZT16), without altering muscle fiber-type distribution, and normalized circadian rhythmicity of core clock genes. Conclusions: Rutin attenuates D-gal-induced cellular senescence by modulating circadian rhythms, reducing oxidative stress, and improving mitochondrial function. Importantly, its in vivo effects on muscle performance and circadian regulation suggest that rutin is a promising therapeutic strategy to counteract skeletal muscle aging and sarcopenia. Full article

This paper analyzes a finite-capacity $GI/M/2/N$ queue with two heterogeneous servers operating under a multiple working-vacation policy, Bernoulli feedback, and customer impatience. Using the supplementary-variable technique in tandem with a tailored recursive scheme, we derive the stationary distributions of the system size as observed at pre-arrival instants and at arbitrary epochs. From these, we obtain explicit expressions for key performance metrics, including blocking probability, average reneging rate, mean queue length, mean sojourn time, throughput, and server utilizations. We then embed these metrics in an economic cost function and determine service-rate settings that minimize the total expected cost via the Bat Algorithm. Numerical experiments implemented in R validate the analysis and quantify the managerial impact of the vacation, feedback, and impatience parameters through sensitivity studies. The framework accommodates general renewal arrivals ($GI$), thereby extending classical ($M/M/2/N$) results to more realistic input processes while preserving computational tractability. Beyond methodological interest, the results yield actionable design guidance: (i) they separate Palm and time-stationary viewpoints cleanly under non-Poisson input, (ii) they retain heterogeneity throughout all formulas, and (iii) they provide a cost–optimization pipeline that can be deployed with routine numerical effort. Methodologically, we (i) characterize the generator of the augmented piecewise–deterministic Markov process and prove the existence/uniqueness of the stationary law on the finite state space, (ii) derive an explicit Palm–time conversion formula valid for non-Poisson input, (iii) show that the boundary-value recursion for the Laplace–Stieltjes transforms runs in linear time $\mathcal{O}\left(N\right)$ and is numerically stable, and (iv) provide influence-function (IPA) sensitivities of performance metrics with respect to $({\mu }_1,{\mu }_2,\nu ,\alpha ,\varphi ,\beta )$. Full article

Consider regression models where the response variable Y only depends on the $p\times 1$ vector of predictors $\mathit{x}={(x_1,\dots ,x_p)}^T$ through the sufficient predictor $SP=\alpha +{\mathit{x}}^T\mathit{\beta }$. Let the covariance vector $\mathrm{Cov}(\mathit{x},Y)={\mathbf{\Sigma }}_{\mathit{x}Y}$. Assume the cases ${({\mathit{x}}_i^T,Y_i)}^T$ are independent and identically distributed random vectors for $i=1,\dots ,n$. Then for many such regression models, $\mathit{\beta }=\mathbf{0}$ if and only if ${\mathbf{\Sigma }}_{\mathit{x}Y}=\mathbf{0}$ where 0 is the $p\times 1$ vector of zeroes. The test of $H_0:{\mathbf{\Sigma }}_{\mathit{x}Y}=\mathbf{0}$ versus $H_1:{\mathbf{\Sigma }}_{\mathit{x}Y}\ne \mathbf{0}$ is equivalent to the high dimensional one sample test $H_0:\mathit{\mu }=\mathbf{0}$ versus $H_A:\mathit{\mu }\ne \mathbf{0}$ applied to ${\mathit{w}}_1,\dots ,{\mathit{w}}_n$ where ${\mathit{w}}_i=({\mathit{x}}_i-{\mathit{\mu }}_{\mathit{x}})(Y_i-{\mu }_Y)$ and the expected values $E(\mathit{x})={\mathit{\mu }}_{\mathit{x}}$ and $E(Y)={\mu }_Y$. Since ${\mathit{\mu }}_{\mathit{x}}$ and ${\mu }_Y$ are unknown, the test of $H_0:\mathit{\beta }=\mathbf{0}$ versus $H_1:\mathit{\beta }\ne \mathbf{0}$ is implemented by applying the one sample test to ${\mathit{v}}_i=({\mathit{x}}_i-\overline{\mathit{x}})(Y_i-\overline{Y})$ for $i=1,\dots ,n$. This test has milder regularity conditions than its few competitors. For the multiple linear regression one component partial least squares and marginal maximum likelihood estimators, the test can be adapted to test $H_0:{({\beta }_{i_1},\dots ,{\beta }_{i_k})}^T=\mathbf{0}$ versus $H_1:{({\beta }_{i_1},\dots ,{\beta }_{i_k})}^T\ne \mathbf{0}$ where $1\le k\le p.$ Full article

Teotihuacan, one of the most significant urban and ceremonial centers of ancient Mesoamerica, was abruptly abandoned in the mid-1st millennium AD. The cause and timing of its collapse—commonly placed between 600 and 650 AD—remain major questions in Mesoamerican archaeology. In this study, we present a new archaeomagnetic investigation of six burned structures distributed along the Street of the Dead, including sites at the Square of the Moon, the Room of Columns, the Northwest Complex of the San Juan River, the Superimposed Buildings, and the West Plaza. Magnetic analyses revealed pseudo-single-domain magnetite as the main remanence carrier and produced well-grouped paleodirections (site-mean declinations ranging from 341.1° to 1.7°, α₉₅ ≤ 3.6°) and reliable absolute paleointensities (ranging from 39.4 ± 3.4 μT to 52.5 ± 5.4 μT), obtained using the Thellier-type double-heating method. Archaeomagnetic dating using both global geomagnetic models (SHAWQ.2k) and regional secular variation curves suggests that the last heating events at these sites occurred between ~400 and 500 AD—well before the traditionally cited Metepec phase (550–650 AD) and the so-called “Great Fire.” These findings challenge the prevailing chronological framework and provide compelling evidence that major episodes of destruction and depopulation may have begun earlier than previously recognized. Full article

Ti–6Al–4V (TC4) dual-phase titanium alloy is widely used in aerospace components owing to its excellent strength-to-weight ratio and high-temperature stability. However, conventional machining often generates a wide heat-affected zone (HAZ) and oxide or recast layers, which deteriorate the microstructure and reduce long-term reliability. In this study, the water-jet-guided laser (WJGL) process was applied to investigate how coupled laser–water interactions influence the groove morphology, elemental distribution, and crystallographic evolution of TC4 alloy. Under optimized parameters, the WJGL process reduced the HAZ width to less than 1 $\mathsf{\mu }$m, effectively removed the resolidified layer, and suppressed surface oxidation. SEM, EDS, and EBSD analyses confirmed that the $\alpha$ + $\beta$ dual-phase structure remained stable, with no significant phase transformation or grain coarsening. Compared with conventional laser cutting, WJGL achieved smoother surfaces, improved interfacial integrity, and reduced thermal damage. These findings highlight the potential of WJGL for precision machining of high-performance titanium alloys and provide theoretical and experimental support for enhancing the microstructural control and service reliability of aerospace TC4 components. Full article

The coefficient of variation α is a critical parameter in the Brownian Passage Time (BPT) model, used to quantify the variability of large earthquake recurrence intervals. In this paper, a new estimation method is proposed for α based on paleoseismic sequences across multiple faults within a given tectonic region. By integrating Monte Carlo simulations with a Bayesian framework, the method assesses the probability distribution of α without assuming that the sample average recurrence interval equals the true mean μ, thereby avoiding epistemic bias. To validate the method, 1,000,000 simulations were conducted in two study areas of differing spatial scales. In the Western Qilian Mountains-Hexi Corridor, the posterior mean of α is 0.36 (without dating uncertainty) and 0.34 (with uncertainty). Expanding the analysis to 29 faults across western China, the estimated α increases to 0.39 (without dating uncertainty) and 0.36 (with uncertainty), with substantially reduced uncertainty bounds. The results reveal that increasing the number of paleoseismic sequences significantly reduces the uncertainty in estimating α, while considering dating uncertainty has only a minor impact. The methodology provides a robust framework for deriving region-specific recurrence variability parameters and proves particularly valuable for tectonically active regions where individual fault records are sparse but collectively form comprehensive datasets across multiple fault systems. Full article

The Iris genus is known for its large blooms and significant conservation value, as well as its horticultural appeal. There are over 300 species of irises, which are widely distributed across the northern temperate zone. Iris palaestina (Baker) Barbey, commonly known as the Lebanese iris, is an endemic species of the Middle East with limited prior phytochemical research. This study was conducted to examine the metabolomic complexity and chemical profile of the flower extract of I. palaestina using advanced analytical tools. Molecular networking was employed to investigate its chemotaxonomy and phytochemical composition. In silico annotation tools—network annotation propagation (NAP), DEREPLICATOR, and MS2LDA—were applied to identify chemical classes and substructures within the extract. The flower extract of I. palaestina was found to contain diverse metabolite classes, including flavonoids, terpenoids, and lipids, with a total of 15 compounds annotated. Subsequent chromatographic separation yielded four major compounds, identified as the isoflavonoid irigenin, the flavonoid embinin, the xanthone mangiferin, and the lipid N-lauryldiethanolamine. Among these, irigenin and mangiferin exhibited significant α-glucosidase inhibitory activity, with IC₅₀ values of 32.1 μM and 36.1 μM, respectively. This study provides the first comprehensive metabolomic characterization of I. palaestina, revealing it as a rich source of bioactive phytochemicals spanning multiple metabolite subclasses. These findings emphasize the possible use of I. palaestina for further pharmaceutical investigation and natural product discovery. Full article

Carpesium cernuum L., the most widespread representative of the genus Carpesium, has been traditionally used in some regions of Asia as a remedy for various ailments or as a vegetable. Although the plant is distributed in Europe, there is no data on its medicinal use in this part of the world. The chemical composition of European Carpesium cernuum L. has remained unknown until now, except for the compositions of essential oils distilled from the roots and aerial parts of the plant. Polyphenolic profiles of hydroalcoholic extracts from C. cernuum were studied using the HPLC-MSⁿ technique. The analysis revealed the presence of 24 hydroxycinnamates, which were dominated by caffeoylquinic and caffeoylhexaric acids. Moreover, fractionation of the chloroform extracts from the plant led to the isolation of three new compounds, 8α-angeloyloxy-4β-hydroxy-5β-(3-methylbutyryloxy)-9-oxo-germacran-6α,12-olide, 9β-angeloyloxy-4β,8α-dihydroxy-5β-(3-methylbutyryloxy)-3-oxo-germacran-6α,12-olide, and a dihydrobenzofuran derivative, together with twelve known compounds. 8-Hydroxy-9,10-diisobutyryloxythymol, a monoterpenoid thymol derivative from the roots of the plant, was evaluated for potential neuroprotective and cytotoxic activities using differentiated and undifferentiated SH-SY5Y neuroblastoma cells. At a concentration range of 1–10 μM, the compound provided partial (up to 50%) protection against H₂O₂-induced cell damage in the undifferentiated cells. At concentrations higher than 25 μM, the monoterpenoid significantly reduced the viability of the cells (IC₅₀: 65.7 μM for the undifferentiated cells and 40.9 μM for the differentiated cells). Full article

The influence of heat input (HI) on the microstructure, microhardness, electrochemical corrosion performance of cold metal transfer additively manufactured (CMTAM) nickel–aluminum bronze alloys was investigated. The nickel–aluminum bronze exhibited an α-Cu austenite matrix with minor γ₂-Cu₉Al₄ and κ phases. As HI increased, the microstructure coarsened progressively. Electron backscatter diffraction (EBSD) analysis revealed that with increasing HI, the grain size gradually increased and the Schmid factor increased. Consequently, the microhardness declined from 198.3 HV to 171.7 HV. The decrease in microhardness with increasing heat input is primarily attributed to the grain coarsening and the coarsening and uneven distribution of the κ phase. As the heat input (HI) increased from 243.8 J/mm to 644.7 J/mm, the corrosion current density rose significantly from 2.56 ± 0.04 μA/cm² to 7.52 ± 0.07 μA/cm². This result indicates a marked deterioration in the material’s corrosion resistance. This phenomenon can be attributed to the grain coarsening and the distribution of Al solute within the microstructure. The CMTAM nickel–aluminum bronze alloys hold significant potential for enhancing the reliability and long-term protection of marine engineering equipment. Full article

This study investigates the impact of wall roughness on the performance of the Non-Line-of-Sight (NLOS) component in Visible Light Communication (VLC) systems designed for underground mining environments, adhering to safety and communication standards such as IEC 60079-28(intrinsic safety in explosive atmospheres) and IEEE 802.15.7 (VLC parameters). Using probabilistic models aligned with the ITU-R P.1238 propagation guidelines, the research evaluates how wall materials (e.g., coal, shale, limestone) and their irregular geometries, characterized by surface roughness profiles compliant with ISO 8503-2,influence reflection coefficients (0.05–0.85 range), incidence angles (0°–90°), and irradiance angles (5°–180°), which are critical for signal propagation. Simulation scenarios, parameterized with material reflectivity data from ASTM E423, explore the effects of statistical distributions (uniform, normal with $\mu$ = 0.3, $\sigma$ = 0.2; exponential $\lambda$ = 2; gamma $\alpha$ = 0.5, $\beta$ = 0.2) on power distribution, channel impulse response, and reflection coefficients. The results indicate variations in maximum received power: a decrease of 80% for uniform distribution, an increase of 150% for exponential distribution, and a 100% increase for gamma distribution in reflection conditions. Under incidence and irradiance conditions, uniform distribution exhibited a 158.62% increase, whereas exponential distribution and gamma distribution experienced reductions of 72.22% and 7.04%, respectively. These variations align with IEC 62973-1 EMI limits and emphasize the role of roughness (Ra = 0.8–12.5 μm per ASME B46.1). Full article

Cosmic rays exhibit anomalous diffusion behaviors in the heliospheric environment that cannot be adequately described by classical diffusion models. In this paper, we develop a theoretical framework employing a fractional Fokker–Planck equation to model the anomalous transport of cosmic rays. This approach accounts for the observed non-Gaussian distributions, long-range correlations and memory effects in cosmic ray fluxes. We derive analytical solutions using the Adomian Decomposition Method and express them in terms of Mittag-Leffler functions and Lévy stable distributions. The model parameters, including the fractional orders $\alpha$ and $\mu$ and the entropic index q, are estimated by a short comparison between theoretical predictions and observational data from cosmic ray experiments. Our findings suggest that the integration of fractional calculus and non-extensive statistics can be employed for describing the cosmic ray propagation and the anomalous diffusion observed in the heliosphere. Full article

Two natural ent-kaurene diterpenoids, ent-15α-angeloyloxykaur-16-en-3β-ol (7) and ent-15α-angeloyloxykaur-16-en-3β,9-diol (8), were extracted from the aerial parts of Distichoselinum tenuifolium, and six new derivatives were synthesised from compound (7). The antitumour properties of these natural and derivative ent-kaurenes (2, 7, 9–13) were evaluated in three cancer cell lines: HT29 (colon cancer), HepG2 (hepatocellular carcinoma), and B16-F10 (murine melanoma). Among them, the synthesised ent-kaurene (13) containing an exomethylene–cyclopentanone moiety showed the strongest antiproliferative effects in all cell lines tested, with significantly lower IC₅₀ values around 2.5 μM. Compounds 13 and 12, together with their precursor (7), were selected for further comparative cytometric and microscopic analyses. Cell cycle studies revealed that derivatives 12 and 13 exhibited promising cytostatic activity by inducing selective G2/M phase arrest, particularly effective in HT29 and HepG2 cells. Conversely, precursor (7) showed no significant effect on B16-F10 cell cycle distribution. The Annexin V-FITC/PI double staining assay confirmed the robust apoptotic effects of compounds (7), 12 and 13, with compound 13 inducing up to 99% total apoptosis and exhibiting significant apoptotic activity in all cell lines tested. These apoptotic effects were closely linked to mitochondrial dysfunction, as evidenced by a marked loss of mitochondrial membrane potential and reduced Rh123 fluorescence in treated cells, thereby activating the intrinsic apoptotic pathway. These findings highlight the critical role of mitochondrial disruption in the cytotoxic mechanisms of these ent-kaurenes and underscore their potential as promising anticancer agents. Full article

In this paper, we present the evaluation of network parameters for the unmanned aerial vehicle (UAV)-enabled triple-hop mixed RF/FSO-based wireless communication system, where one relay is a fixed relay and the second relay is a UAV which acts as decode-and-forward relay for communication from the base station (B) to the multiple mobile users $\left(M_i\right), i\in \{1,2\cdots N\}$. The first hop from B to fixed relay (R1) is the radio frequency (RF) link modeled using $\alpha -\kappa -\mu$ fading distribution, the second hop from the relay R1 to the UAV relay (R2) is a free space optics (FSO) link modeled using Gamma-Gamma fading, and the third hop from R2 to the $M_i$ is, again, an RF link modeled using the Rayleigh fading model. The direct communication between the B and the $M_i$ is not feasible due to the very large distance. We derive the closed form analytical expression for the outage probability of the proposed system and find the effect of the base system parameters on the performance of the system. We also analyze the outage probability of the system at high SNR values to get further insights of the system performance. In addition, altitude optimization of UAV is carried out to know the optimal elevation angle in correspondence with UAV’s optimal altitude in order to maximize performance of the system. Full article

In order to address the problems of low spectrum efficiency in current communication systems and extend the lifetime of energy-constrained relay devices, this paper proposes a novel dual-hop free-space optical (FSO) system that integrates cognitive radio (CR) and energy harvesting (EH). In this system, the source node communicates with two users at the terminal via FSO and terahertz (THz) hard-switching links, as well as a multi-antenna relay for non-orthogonal multiple access (NOMA). There is another link whose relay acts as both the power beacon (PB) in the EH system and the primary network (PN) in the CR system, achieving the double function of auxiliary transmission. In addition, based on the three possible practical working scenarios of the system, three different transmit powers of the relay are distinguished, thus enabling three different working modes of the system. Closed-form expressions are derived for the interruption outage probability per user for these three operating scenarios, considering the Gamma–Gamma distribution for the FSO link, the $\alpha -\mu$ distribution for the THz link, and the Rayleigh fading distribution for the radio frequency (RF) link. Finally, the numerical results show that this novel system can be adapted to various real-world scenarios and possesses unique advantages. Full article

This research presents a closed-form mathematical framework for assessing the performance of a wireless communication system in the presence of multipath fading channels with an instantaneous signal-to-noise ratio (SNR) subjected to the inverse power Lomax (IPL) distribution. It is demonstrated that depending on the channel parameters, such a model can describe both severe and light fading covering most cases of the well-renowned simplified models (i.e., Rayleigh, Rice, Nakagami-m, Hoyt, $\alpha -\mu$, Lomax, etc.). This study provides the exact results for a basic statistical description of an IPL channel, including the PDF, CDF, MGF, and raw moments. The derived representation was further used to assess the performance of a communication link. For this purpose, the exact expression and their high signal-to-noise ratio (SNR) asymptotics were derived for the amount of fading (AoF), outage probability (OP), average bit error rate (ABER), and ergodic capacity (EC). The closed-form and numerical hyper-Rayleigh analysis of the IPL channel is performed, identifying the boundaries of weak, strong, and full hyper-Rayleigh regimes (HRRs). An in-depth analysis of the system performance was carried out for all possible fading channel parameters’ values. The practical applicability of the channel model was supported by comparing it with real-world experimental results. The derived expressions were tested against a numerical analysis and statistical simulation and demonstrated a high correspondence. Full article