Search Results (495)

Urban trees are vital for air pollution mitigation, but their function is often compromised by exposure to particulate matter (PM), which impairs physiological processes and reduces growth. Enhancing tree resilience is therefore essential for maintaining their ecosystem services in polluted urban environments. This study examined the early growth and biochemical responses of Norway spruce (Picea abies) and Norway maple (Acer platanoides) seedlings to foliar PM exposure and assessed whether biochar (BC) soil amendment can alleviate PM-induced stress. Seedlings were cultivated outdoors under three treatments: Control (no treatment), PM (foliar exposure to particulate matter), and PM + BC (PM exposure with 10% biochar added to the substrate). Results revealed that Norway maple showed significant biochemical sensitivity to PM, including substantial reductions in chlorophyll and increases in antioxidant activity. However, Norway spruce showed more moderate pigment changes but reduced height growth. BC modulated oxidative and phenolic responses (TPC, TFC, MDA) and partially mitigated PM-induced stress, although its effectiveness varied by species. For Norway spruce, BC significantly enhanced resilience by restoring height growth, stabilizing pigments, and reducing oxidative stress compared with treatment using PM alone. In contrast, for Norway maple, BC failed to restore chlorophyll levels and increased oxidative and phenolic activity, yielding mixed outcomes. Despite physiological differences between the two species, multivariate PCA consistently showed that PM-treated seedlings diverged from the control cluster, whereas PM + BC-treated seedlings were closer to the controls, with mitigation substantially stronger in Norway spruce. These findings demonstrate that biochar can reduce PM-induced stress, but its successful implementation depends fundamentally on selecting appropriate species traits and understanding their specific metabolic response strategies. Full article

Deep planting of young trees—defined as the burial of the root collar below soil grade—is widely recognized by practitioners as an improper technique that can impair tree development and establishment. Despite this knowledge, research has shown that urban trees are frequently planted too deeply. To better understand the impacts of planting depth on the urban forest, we conducted a literature review of peer-reviewed and professional studies relevant to the effects of planting depth in urban trees. Most studies reported effects on tree establishment (34%), growth (23%), and root development (22%). A general conclusion across reviewed articles was evident: trees planted too deep exhibited higher mortality, slower establishment, and reduced growth, primarily due to poor root development. Effects of planting depth were also species-specific—Norway Maple (Acer platanoides L.), Turkish Hazel (Corylus colurna L.), White Ash (Fraxinus americana L.), and Green Ash (Fraxinus pennsylvanica Marshall) showed minimal differences in performance when deeply planted, while Baldcypress (Taxodium distichum L. Rich), which tolerates anoxic conditions, performed better at or below grade than when planted above grade, although the findings in these studies only measured the effects of planting depth relative to limited measured parameters. We also compiled a reference table that links tree species to their performance based on planting depth. These findings highlight the critical role of planting depth in shaping root architecture and long-term success, emphasizing the need for adherence to best practices concerning proper planting, tree maintenance (e.g., mulching), and production in the nursery. Full article

Acer saccharum Marsh., valued for its ornamental, material, and edible uses, is an important temperate tree species in the Northern Hemisphere. A blight disease affecting branches of A. saccharum was first identified in 2023 in Shandong, China. The causal agent was identified as Botryosphaeria wangensis G.Q. Li & S.F. Chen based on cultural and morphometric characteristics. Phylogenetic analysis was performed by amplifying and sequencing the internal transcribed spacer (ITS) region of rDNA, the translation elongation factor 1α (tef1) partial gene, the β-tubulin (tub2) partial gene, and the second largest subunit of RNA polymerase II (rpb2), in combination with morphological data. Symptoms observed in the field were replicated in a pathogenicity test through inoculation of A. saccharum branches, thus satisfying Koch’s postulates. To our knowledge, this is the first report worldwide of B. wangensis infecting A. saccharum. Full article

Abdominal fat deposition is an important economic trait in poultry, as excessive accumulation reduces feed efficiency and carcass yield. The gut microbiota is known to influence host energy metabolism and fat storage, suggesting its potential involvement in fat deposition. This study examined the relationship between intestinal microbiota and abdominal fat deposition in an F₂ population derived from Cherry Valley Ducks (♂) × Runzhou Crested White Ducks (♀) at 42 days of age. Based on abdominal fat rate, ducks with values of 0–0.75% and 1.5–2.25% were defined as the low (LF) and high (HF) abdominal fat groups, respectively. A combined multi-omics approach was used, including 16S rRNA gene sequencing, metagenomics, and whole transcriptomics, to compare high and low abdominal fat rate groups. 16S rRNA gene sequencing results showed that the cecum had the highest microbial diversity among all intestinal segments (duodenum, jejunum, ileum, and rectum) and was significantly enriched in carbohydrate metabolism pathways, highlighting its key role in nutrient utilization and growth. Therefore, the cecum was selected for further analysis. Metagenomic analysis of the cecum contents revealed significantly different intestinal microbial β diversity between the high and low abdominal fat rate groups (p < 0.05). The low abdominal fat rate group was enriched in beneficial microorganisms such as Paenibacillus, Butyrivibrio, Coprococcus, Ruminococcaceae, Veillonellaceae (Clostridiales), and Firmicutes. Conversely, the high abdominal fat rate group was characterized by an increased abundance of Bacteroidetes, including both beneficial and potentially pathogenic taxa such as Alistipes and Eggerthellales. The integrated analysis of metagenomic and whole transcriptome sequencing showed that Firmicutes and Bacteroidetes were not only related to energy metabolism, lipid metabolism, and amino acid metabolism, but also to the expression of FGF2, FKBP5, PNPLA2, PLIN3, FGFR2, DGAT2, and ACER2. In addition, Firmicutes and Bacteroidetes were also associated with 7 lncRNAs: XR_003493494.1, XR_003492471.1, XR_001190174.3, TCONS_00005095, XR_001190238.3, TCONS_00005095, and XR_003492841.1. In conclusion, this study highlights that the cecal microbiota is closely associated with abdominal fat deposition in ducks, elucidating its potential influence on host metabolism and gene expression. These findings enhance our understanding of the gut microbiota’s relationship with obesity and offer new strategies to modulate gut–microbe interactions to reduce abdominal fat accumulation in poultry. Full article

Acer truncatum Bunge exhibits remarkable cold tolerance at the mature seedling stage, yet the mechanisms governing its seed germination under low-temperature conditions remain poorly understood. To elucidate the molecular and physiological mechanisms underlying low-temperature germination in A. truncatum seeds, we selected A. truncatum seeds as the experimental material. The seeds were evenly divided into two groups and subjected to germination under 25 °C (control) and 4 °C (low-temperature stress) conditions, followed by transcriptome sequencing and physiological and biochemical analyses. Transcriptome sequencing analyzed differential genes and physiological indicators. Fourteen transcription factor families were identified (ARR-B, AP2-EREBP, bHLH, NAC, FAR1, MADS, WRKY, AB13VP1, bZIP, C3H, CROS, LOB, TCP, and SBP). These regulate seed germination under abiotic/biotic stress. GO term enrichment occurred in biological processes. KEGG enrichment involved carbon metabolism, the glutathione pathway, the citrate cycle, and glycolysis. Most genes were upregulated. Citrate cycle and glycolysis correlated with seed activity, promoting germination. The glutathione cycle greatly improves the stress resistance of seed germination. There were 1804 genes that were upregulated and 8075 genes that were downregulated during seed germination. Among differential genes, CBF 5 was significantly downregulated but most WRKY families and LEA14-A were upregulated to maintain cell homeostasis. Meanwhile, GSH, SOD, POD, and proline (Pro) levels increased with prolonged stress. MDA rose initially, then declined. Soluble protein content first increased, then decreased, but remained higher than controls. Seeds germinated under low temperature, but germination potential was slightly lower than at room temperature. We propose that LEA protein, antioxidant enzymes, and Pro accumulation enhance cold tolerance. This study elucidates the physiological and molecular mechanisms underlying seed germination, advancing the understanding of cold tolerance in A. truncatum. Full article

Introduction: The Mediterranean diet (MD) has been linked to better cognition, but evidence in older adults at high dementia risk is limited. Moreover, the traditional Mediterranean Diet Adherence Screener (MEDAS) counts daily wine consumption as a beneficial component, which may distort genuine diet–cognition relationships. Objective: Evaluate whether MD adherence, as measured with the original MEDAS (MEDAS-O) versus a version that reverses the wine item (MEDAS-R), is associated with cognitive function in Portuguese adults aged 55–85 years at increased dementia risk. Methodology: The sample comprised 75 participants from the NUTRIMIND randomised controlled trial (mean age 70.5 ± 7.0 years). MD adherence was evaluated using the original version of MEDAS (MEDAS-O) and an adapted version with a reverse score in the wine question (MEDAS-R). Cognitive function was assessed via the Montreal Cognitive Assessment (MoCA), Addenbrooke’s Cognitive Examination Revised (ACE-R) and Mini-Mental State Examination (MMSE). Statistical analysis was performed using Analysis of Covariance (ANCOVA) models adjusted for age, sex, BMI, education, and physical activity. Results: MEDAS-R was positively associated with better MMSE performance (p = 0.043) and showed a borderline association with the MoCA (p = 0.051), but not with the ACE-R score (p = 0.356). No association was found between MEDAS-O and cognitive function. Better cognitive scores were more frequently observed among participants with higher education (p < 0.001). Conclusions: Reversing the wine item changes how MEDAS relates to cognitive function. These findings support re-evaluating how wine is scored in MD adherence measures. Full article

This paper explores the dynamic behavior of different wood species in the form of violin boards, based on experimental modal analysis using a single-input, multiple-output configuration. Thus, two groups of species were studied: the first group for the violin top plates, being analyzed Picea abies (spruce), Taiwania cryptomerioides Hayata (Taiwania), and Cryptomeria japonica (Japanese cedar), and the second group, with species for the back plates, such as Acer pseudoplatanus (maple), Populus nigra (poplar), Salix alba (willow), and Firmiana simplex (Chinese parasol). The results highlighted the frequency spectrum and the dominant resonance frequency, as well as the frequency damping, the signal processing analysis being based on Fast Fourier Transform and Wigner–Ville distribution of signals. The results highlighted that the lowest values of acoustic radiation are recorded for maple wood (7.8 m⁴ kg⁻¹ s⁻¹) and Taiwania (10.08 m⁴ kg⁻¹ s⁻¹), and the highest values for spruce (14.7 m⁴ kg⁻¹ s⁻¹) and Chinese parasol (15.58 m⁴ kg⁻¹ s⁻¹). Regarding the resonance frequency, the Taiwania and Japanese cedar plates present the dominant frequency around 600–635 Hz in comparison with Norway spruce having 920 Hz. The ratios between dominant frequencies of the Chinese parasol, poplar, maple, and willow are 1:1.42:2.62:2.98. It can be concluded that spruce and maple wood present the best dynamic properties, but when using other species, Japanese cedar wood for the top plate and Chinese parasol wood for the back plate represent species with potential in the construction of stringed musical instruments. Either a mechano-thermal treatment or an appropriate finish can enhance the acoustic qualities of these wood species, research that can be undertaken in the future. Full article

In this study, we developed three forest management scenarios for Simcoe County Forest in Southern Ontario, Canada, using the Physiological Principles Predicting Growth (3-PG) model to simulate future forest growth and carbon dynamics. The focus was on four main species: Red pine (Pinus resinosa), White pine (Pinus strobus), Sugar maple (Acer saccharum), and Red oak (Quercus rubra). We parameterized, calibrated, and validated parameters of the 3-PG model for these four species and applied the model to evaluate the performance of management scenarios incorporating timber and carbon values in Simcoe County Forest. The first scenario, “business as usual,” maintained the existing management plan for the forest, ensuring stable timber income (531.2 CAD/hectare) and moderate carbon sequestration. The second scenario aimed to optimize management for the highest timber Net Present Value (NPV), with half of the trees harvested before 2030, followed by gradual thinning over 15 years. This approach yielded the highest financial returns (1634.1 CAD/hectare) but the lowest carbon sequestration potential. The third scenario integrated carbon certification, emphasizing the retention of sugar maple stands over the next 20 years. This scenario produced financial returns (580.2 CAD/hectare) higher than the “business as usual” scenario, while saving 49.33 tons of biomass per hectare. Overall, this study provides a reference for the implementation of carbon sequestration projects. Full article

Northern hardwood forests of the Lake Superior region face a series of novel disturbance pressures including canopy dieback. Previous studies have linked regional sugar-maple (Acer saccharum) canopy dieback to introduced earthworms, which may have coinciding impacts on the ground-layer plant community. Dieback–earthworm interactions may lead to important longer-term changes in forest structure and function, but these relationships but have not been characterized. We sampled ground-layer plant communities in five national forest units in Michigan, Wisconsin, and Minnesota in 2010, and again just over a decade later in 2021. Non-metric multidimensional scaling ordination and indicator species analysis were used to assess relationships among ground-layer community composition and structure, functional traits, and environmental gradients including forest-floor condition and A. saccharum canopy dieback. Increases in dieback and earthworm disturbance in the decade between inventories were accompanied by a marked divergence in observed ground-layer plant community structure between national forests. Ordinations of 2021 data indicated a strengthening relationship between forest-floor condition and earthworm abundance. Our results suggest that earthworm impacts and A. saccharum dieback are driving changes in the ground layer on broad geographic and temporal scales, with short- and long-term implications for plant-community structure and function, and higher trophic levels. Full article

Urban expansion alters environmental conditions, influencing tree physiology and performance. Urban trees provide cooling, sequester carbon, support biodiversity, filter contaminants, and enhance human health. This study examines how two common urban trees—Norway Maple (Acer platanoides L.) and Little-leaved Linden (Tilia cordata Mill.)—respond to urban site conditions by assessing leaf morphology, stomatal, and gas exchange traits across street and urban park sites in Chicago, IL. Street trees exhibited structural trait adjustments, including smaller leaf area, reduced specific leaf area, and increased stomatal density, potentially reflecting acclimation to more compact and impervious conditions. Norway Maple showed stable photosynthetic assimilation (A), stomatal conductance (g_s), and transpiration (E) across sites, alongside higher intrinsic water-use efficiency (_iWUE), indicating a conservative water-use strategy. In contrast, Little-leaved Linden maintained A and g_s but showed elevated E and _iWUE at street sites, suggesting adaptive shifts in water-use dynamics under street microenvironments. These findings highlight how species-specific physiological strategies and local site conditions interact to shape tree function in cities and underscore the importance of incorporating functional traits into urban forestry planning to improve ecosystem services and climate resilience. Full article

Facial recognition systems are increasingly used for authentication across domains such as finance, e-commerce, and public services, but their growing adoption raises significant concerns about spoofing attacks enabled by printed photos, replayed videos, or AI-generated deepfakes. To address this gap, we introduce a multi-layered Face Recognition-as-a-Service (FRaaS) platform that integrates passive liveness detection with active challenge–response mechanisms, thereby defending against both low-effort and sophisticated presentation attacks. The platform is designed as a scalable cloud-based solution, complemented by an open-source SDK for seamless third-party integration, and guided by ethical AI principles of fairness, transparency, and privacy. A comprehensive evaluation validates the system’s logic and implementation: (i) Frontend audits using Lighthouse consistently scored above 96% in performance, accessibility, and best practices; (ii) SDK testing achieved over 91% code coverage with reliable OAuth flow and error resilience; (iii) Passive liveness layer employed the DeepPixBiS model, which achieves an Average Classification Error Rate (ACER) of 0.4 on the OULU–NPU benchmark, outperforming prior state-of-the-art methods; and (iv) Load simulations confirmed high throughput (276 req/s), low latency (95th percentile at 1.51 ms), and zero error rates. Together, these results demonstrate that the proposed platform is robust, scalable, and trustworthy for security-critical applications. Full article

Aging is characterized by a progressive decline in physiological function that impairs performance and increases vulnerability to disease and mortality. Delaying this deterioration is key to promoting healthy aging. Age-associated functional decline is closely linked to alterations in intermediary metabolism, including disrupted lipid metabolism and impaired mitochondrial function. Counteracting these metabolic changes, particularly those affecting basal metabolic rate and energy utilization, may be a feasible strategy to extend healthspan. The Renin-Angiotensin System (RAS), which controls blood pressure through Angiotensin II, an octapeptide hormone generated from Angiotensin I by Angiotensin-Converting Enzyme (ACE), has been identified as a potential target for aging therapies. ACE inhibitors, such as the commonly prescribed vasodilator lisinopril, have been shown to exert beneficial effects on healthspan. Disentangling their systemic effects from direct cellular actions on intermediary metabolism is challenging in humans but can be pursued in model organisms. Drosophila melanogaster expresses two ortholog of mammalian ACE, Ance and Acer, which have diverged to acquire different functions. Since fundamental cellular processes are evolutionarily conserved and flies have an open circulatory system, Drosophila provides a versatile model for translational studies on ACE inhibition and aging. Recent studies in Drosophila reveal sex-, age-, and genetic background-specific effects of lisinopril on metabolic rates and aging-related organismal phenotypes. Integrating preclinical findings from Drosophila with clinical studies will be essential to define the therapeutic potential of RAS inhibition in extending lifespan and delaying aging. Full article

The global forestry discourse frequently highlights the issue of ungulate browsing, which can significantly impact tree regeneration and tree species composition by inhibition of growth and elimination of certain, particularly ecologically valuable, tree species. The forestry field often utilizes the percentage of browsed trees within a specific area, ranging from single hunting grounds to broader provincial scales, as a metric of browsing intensity. This measure correlates with ungulate density, which is known to vary across landscapes, rendering spatially averaged browsing percentages less useful for silvicultural decisions even with accurate results. Addressing this gap, we utilized a GLMM with random effects to assess tree specific browsing pressure more appropriately. We incorporated data from two adjacent areas in the northeastern limestone Alps, focussing on the four important tree species in the region (Abies alba, Acer pseudoplatanus, Fagus sylvatica, and Picea abies). We analyzed data collected with distinct methodologies for the two regions, respectively, Austrian Federal Game Impact Monitoring and Austrian Regeneration and Browsing Monitoring of Federal Forests. Overall, the data documented browsing occurrence on 8933 trees over 632 sampling plots totalling 55,000 hectares. By comparing various models, including those with spatial considerations, we found that treating sampling plot location as a latent state variable improved the model fit and allowed prediction of browsing probability on a landscape scale. This study underlines the value of incorporating spatial elements into models for assessing browsing pressure and its spatial variations, thereby facilitating more informed silvicultural decisions. Full article

Fallen leaves of Norway maple (Acer platanoides L.) were evaluated as a lignocellulosic substrate for anaerobic digestion (AD). The aim was to identify the pretreatment variant that maximizes methane yield. Leaves were milled and dry sieved, and two particle size classes were selected. The 1–2 cm fraction underwent chemical pretreatment by ozonation only, whereas the 0.5–1 cm fraction was first ozonated and then subjected to biological pretreatment with stepwise dosing of an enzymatic preparation. Ozonation conditions were varied with respect to particle size, ozone concentration (O₃), gas flow rate, and contact time. Reducing the particle size from 1–2 cm to 0.5–1 cm increased the cumulative biogas yield by 9.7% (from 64.73 to 71.01 mL g⁻¹ VS) and the methane yield by 13.6% (from 42.01 to 47.75 mL g⁻¹ VS) relative to the control. These findings indicate that particle size reduction combined with ozonation and staged enzymatic dosing is a promising route to improve biomethane recovery from autumn leaf feedstocks. Full article

Urban tree biodiversity represents a valuable natural resource. However, some fast-growing tree species with limited esthetic value play an important ecological role by colonizing degraded areas, such as closed landfills. Our observations indicate that trees like Betula pendula (Roth), Acer negundo (L.), and Populus tremula (L.) reached the size of adult trees in less than 30 years after the landfill’s closure in the 1990s, forming a nature area similar to a natural forest. A resident survey conducted among the inhabitants of Zgierz confirmed that the lack of space provides opportunities for various forms of recreation. The example analyzed indicates a trend that can be replicated in other cities with minimal human intervention and low financial costs for landfill reclamation. The case study presents an ecological approach to managing degraded sites, where nature determines the quality of the soil environment by eliminating pollutants from the residential surroundings. Furthermore, the research framework provides a basis for developing future models for cleaning up urban landfill sites and promoting placemaking. This pilot study shows a model for old landfills in Europe with well-developed spontaneous vegetation that can be transformed into recreation and sports facilities in the urban areas with industrial past times. Full article