Search Results (144)

Chrysin is a dietary flavonoid with antioxidant and anti-inflammatory activity, but its clinical potential is limited by poor oral bioavailability. This randomized double-blind three period crossover trial evaluated the pharmacokinetics of a novel micellar chrysin formulation co-encapsulated with quercetin and rutin (LMC) compared with a non-micellar chrysin formulation (NMC) and unformulated chrysin (UFC). Secondary objectives included in vitro permeability (Caco-2) and a 30-day safety assessment of daily LMC supplementation. Sixteen healthy adults received a single oral dose of each formulation in randomized order separated by a 7-day washout. Plasma chrysin was quantified over 24 h to determine pharmacokinetic parameters. In vitro Caco-2 assays evaluated permeability, and clinical biochemistry of 15 participants were assessed weekly during 30 days of daily LMC use. LMC achieved >2-fold higher systemic exposure than unformulated chrysin (AUC_0–24 = 914.8 ± 697.5 ng·h/mL; C_max = 87.3 ± 59.4 ng/mL; both p < 0.05) and >2.6-fold higher than NMC, supported by >10-fold higher in vitro permeability. Daily LMC supplementation was well tolerated, with only mild, reversible adverse events and no clinically relevant safety changes, despite higher systemic exposure. Small, but significant, reductions in fasting glucose were observed in both sexes. The novel micellar chrysin–quercetin–rutin formulation substantially improved bioavailability and was well tolerated during 30 days of daily use, supporting its potential as an advanced delivery strategy for flavonoids with poor oral absorption and identifying glucose regulation as a physiological effect of interest. Full article

Floods are among the most frequent and damaging natural hazards in the Mekong River Basin, where the interplay of monsoon-driven climate variability, complex topography, and rapid socio-economic change creates high exposure and vulnerability. This study presents a comprehensive assessment of flood disaster patterns, loss distribution, and regional disparities across five countries in the Lower Mekong Basin—Cambodia, Laos, Myanmar, Thailand, and Vietnam. Using multivariate spatiotemporal analysis based on EM-DAT, MRC, and national government datasets, the study quantifies flood frequency, casualties, and affected population to reveal cross-country differences in disaster impact and timing. Results show that while Vietnam and Thailand experience high flood frequency and storm-induced events, Laos and Cambodia face riverine flooding under constrained economic and infrastructural conditions. The findings highlight a basin-wide increase in flood frequency over recent decades, driven by climate change, land use transitions, and uneven development. The analysis identifies critical gaps in adaptive governance, particularly the need for dynamic policy frameworks that can adjust to spatial disparities in flood typologies (e.g., Vietnam’s storm floods vs. Cambodia’s riverine floods) and improve transboundary coordination of reservoir operations. Despite the region’s extensive reservoir capacity, most infrastructure prioritizes hydropower over flood mitigation. The study evaluates the role of regional cooperation frameworks such as the Lancang–Mekong Cooperation (LMC), demonstrating how strengthened institutional flexibility and knowledge-sharing mechanisms could enhance progress toward Sustainable Development Goals (SDGs) related to water governance (SDG 6), resilient infrastructure (SDG 9), and disaster risk reduction (SDG 11). By constructing the first integrated national-level flood disaster database for the basin and conducting comparative analysis across countries, this research provides empirical evidence to support differentiated yet coordinated flood risk governance strategies at both national and transboundary levels. Full article

Background: Platelet-rich fibrin (PRF) is an autologous platelet concentrate (APC) produced through blood centrifugation. Despite the development of various centrifugation systems, protocol variability continues to pose challenges in selecting the optimal method. This study investigated the effects of three different centrifuges and collection tubes on the fibrin characteristics and growth factor release in leukocyte- and platelet-rich fibrin (L-PRF) and advanced platelet-rich fibrin plus (A-PRF+). Methods: Blood samples from six healthy female volunteers were processed using three centrifuges (Duo, IntraSpin, and LMC-3000) and three collection tubes (Pyrex, A-P, and silica-coated plastic) under high- (~700× g for 12 min) and low-speed (~200× g for 8 min) protocols. Fibrin clot weight and length were assessed. Growth factor release of platelet-derived growth factor-BB (PDGF-BB) and vascular endothelial growth factor (VEGF) was quantified using ELISA. Fibrin architecture was examined via scanning electron microscopy (SEM). Results: High-speed protocols tended to produce larger clots, whereas low-speed protocols generated smaller but more biologically active matrices. The IntraSpin and Duo centrifuges yielded greater clot dimensions and higher growth factor release than the LMC-3000. While tube type had no significant effect on growth factor levels, silica-coated tubes tended to produce the largest clots. The Pyrex tubes demonstrated comparable or superior growth factor release. Conclusions: PRF quality is influenced by centrifuge design, g-force, and tube material. Low-speed protocols with certified centrifuges are recommended, and FDA-approved glass tubes may provide a reliable alternative to reduce silica-related risks. Standardization and appropriate material selection are essential for consistent, safe, and effective regenerative outcomes. Full article

We present a comprehensive information-theoretic evaluation of three widely used rigid water models (TIP3P, SPC, and SPC/$\epsilon$) through systematic analysis of water clusters ranging from single molecules to 11-molecule aggregates. Five fundamental descriptors—Shannon entropy, Fisher information, disequilibrium, LMC complexity, and Fisher–Shannon complexity—were calculated in both position and momentum spaces to quantify electronic delocalizability, localization, uniformity, and structural sophistication. Clusters containing 1, 3, 5, 7, 9, and 11 molecules (denoted 1 M, 3 M, 5 M, 7 M, 9 M, and 11 M) were selected to balance computational tractability with representative scaling behavior. Molecular dynamics simulations validated the force fields against experimental bulk properties (density, dielectric constant, self-diffusion coefficient), while statistical analysis using Shapiro–Wilk normality tests and Student’s t-tests ensured robust discrimination between models. Our results reveal distinct scaling behaviors that correlate with experimental accuracy: SPC/$\epsilon$ demonstrates superior electronic structure representation with optimal entropy–information balance and enhanced complexity measures, while TIP3P shows excessive localization and reduced complexity that worsen with increasing cluster size. The transferability from clusters to bulk properties is established through systematic convergence of information-theoretic measures toward bulk-like behavior. The methodology establishes information-theoretic analysis as a useful tool for comprehensive force field evaluation. Full article

Background: Policymakers in Korea are calling for Local Medical Centers (LMCs) to address regional healthcare disparities by expanding their roles beyond safety-net functions yet often overlook local community perspectives. Methods: Face-to-face survey data collected in 2022 from 2057 adults residing in Chungcheongnam-do were analyzed in this study, using Importance–Performance Analysis to assess how residents of large cities, non-large cities, and rural areas prioritize nine LMC functions. Results: While all valued public health policy and infectious disease control amid COVID-19, notable regional variations appeared: non-large city residents prioritized unmet healthcare needs and operational efficiency, rural respondents emphasized post-discharge care coordination due to aging and chronic disease, and large city residents focused on safety-net roles. Staff training and medical innovation ranked lowest across regions. Conclusions: The results highlight the inadequacy of one-size-fits-all policies and the importance of regionally tailored, resident-informed strategies for equitable public health in Korea. Full article

Urban mobility plays a vital role in shaping sustainable cities, yet the effectiveness of public transportation is often undermined by poor last-mile connectivity (LMC). In the National Capital Region (NCR) of Delhi, despite the Delhi Metro Rail serving as a key transit system, limited integration with surrounding areas hinders accessibility, which particularly affects women, elderly adults, and socioeconomically disadvantaged groups. This study evaluates LMC performance at two key metro stations, Nehru Place and Botanical Garden, using a mixed-methods approach that includes user surveys, spatial survey, thematic analysis, and infrastructure scoring across five critical pillars: accessibility, safety and comfort, intermodality, service availability, and inclusivity. The findings communicate notable contrasts. Botanical Garden exhibits strong intermodal linkages, pedestrian-friendly design, and supportive signage, while Nehru Place indicates a need for infrastructural improvements, safety advancement and upgrades, and strengthened universal design features. These disparities limit effective metro usage and discourage a shift from private to public transport. The study highlights the importance of user-centered, multimodal solutions and the need for cohesive urban governance to address LMC gaps. By identifying barriers and opportunities for improvement, this research paper contributes to the formulation of more inclusive and sustainable urban transport strategies in Indian metropolitan regions. Full article

Background: Hemiplegic cerebral palsy (HCP) is a motor dysfunction disorder resulting from perinatal developmental brain injury, predominantly impairing upper limb function in children. Nonetheless, there has been insufficient research on the brain activation patterns and inter-brain coordination mechanisms of HCP children when performing motor control tasks, especially in contrast to children with typical development(CD). Objective: This cross-sectional study employed functional near-infrared spectroscopy (fNIRS) to systematically compare the cerebral blood flow dynamics and brain network characteristics of HCP children and CD children while performing upper-limb mirror training tasks. Methods: The study ultimately included 14 HCP children and 28 CD children. fNIRS technology was utilized to record changes in oxygenated hemoglobin (HbO) signals in the bilateral prefrontal cortex (LPFC/RPFC) and motor cortex (LMC/RMC) of the subjects while they performed mirror training tasks. Generalized linear model (GLM) analysis was used to compare differences in activation intensity between HCP children and CD children in the prefrontal cortex and motor cortex. Finally, conditional Granger causality (GC) analysis was applied to construct a directed brain network model, enabling directional analysis of causal interactions between different brain regions. Results: Brain activation: HCP children showed weaker LPFC activation than CD children in the NMR task (t = −2.032, p = 0.049); enhanced LMC activation in the NML task (t = 2.202, p = 0.033); and reduced RMC activation in the MR task (t = −2.234, p = 0.031). Intragroup comparisons revealed significant differences in LMC activation between the NMR and NML tasks (M = −1.128 ± 2.764, t = −1.527, p = 0.025) and increased separation in RMC activation between the MR and ML tasks (M = −1.674 ± 2.584, t = −2.425, p = 0.031). Cortical effective connectivity: HCP group RPFC → RMC connectivity was weaker than that in CD children in the NMR/NML tasks (NMR: t = −2.491, p = 0.018; NML: t = −2.386, p = 0.023); RMC → LMC connectivity was weakened in the NMR task (t = −2.395, p = 0.022). Conclusions: This study reveals that children with HCP exhibit distinct abnormal characteristics in both cortical activation patterns and effective brain network connectivity during upper limb mirror training tasks, compared to children with CD. These characteristic alterations may reflect the neural mechanisms underlying motor control deficits in HCP children, involving deficits in prefrontal regulatory function and compensatory reorganization of the motor cortex. The identified fNIRS indicators provide new insights into understanding brain dysfunction in HCP and may offer objective evidence for research into personalized, precision-based neurorehabilitation intervention strategies. Full article

This study proposes a couple of analytical solutions that characterize the anisotropic dense celestial bodies within the Rastall-Rainbow theoretical framework. The analysis assumes a static spherically symmetric matter distribution and derives the corresponding modified field equations. By utilizing well-established radial metric functions and merging them with the two principal pressures, we obtain differential equations related to the time component. Subsequently, we perform the integration of these equations to determine the remaining geometric quantity that encompasses various integration constants. The proposed interior solutions are then matched with the Schwarzschild exterior metric at the boundary of the compact object, facilitating the determination of the constants. Additionally, the incorporation of the non-minimal coupling parameter into these constants is accomplished by enforcing the null radial pressure at the boundary. Afterwards, we rigorously examine the physical characteristics and critical stability conditions of the formulated models under observational data from two pulsars, say 4U 1820-30 and LMC X-4. It is concluded that our models are well-aligned with essential criteria required to ensure the physical viability of stellar structures, subject to specific parametric values. Full article

Understanding the interplay between plant leaf functional traits and plant and soil factors under different soil thicknesses is significant for quantifying the interaction between plant growth and the environment. However, in the context of ecological restoration of vegetation in mining areas, there has been a lot of research on trees, shrubs, and grasses, but the characteristics and correlations of leaf functional traits of vines have not been fully studied to a large extent. Here, we report the differences in leaf functional traits of six vine plants (Parthenocissus quinquefolia, Pueraria lobata, Hedera nepalensis, Campsis grandiflora, Mucuna sempervirens, and Parthenocissus tricuspidata) with distinct growth forms in different soil cover thicknesses (20 cm, 40 cm, and 60 cm). In addition, soil factor indicators under different soil cover thicknesses were measured to elucidate the linkages between leaf functional traits of vine plants and soil factors. We found that P. lobata showed a resource acquisition strategy, while H. nepalensis demonstrated a resource conservation strategy. C. grandiflora and P. tricuspidata shifted toward more conservative resource allocation strategies as the soil cover thickness increased, whereas M. sempervirens showed the opposite trend. In the plant trait–trait relationships, there were synergistic associations between specific leaf area (SLA) and leaf nitrogen content (LNC); leaf moisture content (LMC) and leaf nitrogen-to-phosphorus ratio (LN/P); and leaf specific dry weight (LSW), leaf succulence degree (LSD), and leaf dry matter content (LDMC). Trade-offs were observed between SLA and LSW, LSD, and LDMC; between leaf phosphorus content (LPC) and LN/P; and between LMC, LSW, and LDMC. In the plant trait–environment relationships, soil nutrients (pH, soil total phosphorus content (STP), and soil ammonium nitrogen content (SAN)) and soil enzyme activities (cellulase (CB), leucine aminopeptidase (LAP), enzyme C/N activity ratio, and enzyme N/P activity ratio) were identified as the primary drivers of variation in leaf functional traits. Interestingly, nitrogen deficiency constrained the growth of vine plants in the mining area. Our study revealed that the responses of leaf functional traits of different vines under different soil thicknesses have significant species specificity, and each vine shows different resource acquisition and conservation strategies. Furthermore, soil cover thickness primarily influences plant functional traits by directly affecting soil enzyme activities and nutrients. However, the pathways through which soil thickness impacts these traits differ among various functional traits. Our findings provide a theoretical basis and practical reference for selecting vine plants and optimizing soil cover techniques for ecological restoration in mining areas. Full article

This paper presents an online efficiency optimization strategy for a six-phase induction generator (6PIG) operating in both healthy and faulty modes for micro-hydropower applications. The proposed method is based on an extended Loss Model Control (LMC) approach, in which the direct axis stator current Id is dynamically optimized in real time to minimize the total electrical losses. Unlike conventional LMC strategies, this method explicitly incorporates switching losses into the loss model, along with stator and rotor copper losses and iron losses. The optimization problem is solved using a numerical minimization routine, allowing the control system to adapt continuously to variations in torque requests. The proposed approach is validated under both healthy and faulty configurations of the 6PIG. It is implemented and tested through simulation in MATLAB/Simulink^® and experimentally validated on a 24 kW squirrel cage six-phase induction generator (SC6PIG). The results are compared in terms of power losses, energy saving, and efficiency. Full article

Sustainable management of soils degraded by heavy metals is a major environmental challenge. The aim of this study was to evaluate the acclimatization ability of the hybrid Populus nigra L. × Populus maximowiczii under variable soil moisture conditions. In a greenhouse experiment, it was shown that both soil moisture level and the presence of metals significantly affected plant growth and metabolism. The hybrid showed high nickel (Ni) accumulation at low and medium soil moisture content (LMC, MMC) (BCF 4.56 and 4.99), while copper (Cu) accumulation was highest at MMC (BCF 5.53). Nickel translocation to aerial parts increased after exposure (TF up to 0.63), while Cu translocation was limited (TF below 0.94). Increased humidity promoted the biosynthesis of low molecular weight organic acids (LMWOAs) in roots, with the highest total content recorded in the Cu treatment under high soil moisture content (HMC) (230 μg g⁻¹ FW). In the stems, the highest levels of sum LMWOAs were found under HMC conditions (6764 μg g⁻¹ FW in the control sample), while among the phenolic acids, the highest content of chlorogenic acid (~144 μg g⁻¹ FW) was determined under LMC conditions under Ni stress, which indicates a strong defense response of the plant. The obtained results emphasize the importance of selecting appropriate water conditions in remediation strategies and indicate that the tested poplar hybrid may be a promising tool in improving the quality of degraded soils. Full article

The interface morphology plays an important role in the mechanical properties of laminated metal composites (LMCs). In this study, binary LMCs with different crystallographic characteristics, namely Fe/Al (BCC/FCC), Ni/Al (FCC/FCC), and Mg/Al (HCP/FCC), were fabricated through the hierarchical roll-bonding process. The influence of interface morphology on the mechanical properties of the binary LMCs was investigated systematically. The results show that the strength–hardness coefficient (R) decreases with increasing interface morphology factor (α) for the LMCs, indicating that the strengthening effect of LMCs decreases with increased curvature of the interface. The experimental results reveal that α increases with the increase in rolling deformation (thickness reduction) for the LMCs, which is consistent with the finite element simulation results. The dependence of mechanical properties on interface morphology is mainly related to the microstructural inhomogeneity caused by localized deformation in the harder layer, including the formation of shear bands and variations in grain morphology, size, and orientation, which can lead to stress concentration in the necking zone. Full article

This review examines the prevalence, molecular mechanisms, and clinical implications of RAS mutations in Central Nervous System (CNS) tumors, with a particular focus on glioblastoma. We summarize the current understanding of RAS-driven oncogenic pathways, their contribution to tumor progression, and potential therapeutic strategies targeting RAS and its downstream effectors. Although direct RAS mutations are rare in primary CNS tumors, alterations in RAS signaling, such as NF-1 loss and aberrant receptor tyrosine kinase activation, contribute to malignant progression. Furthermore, emerging evidence links RAS mutations to brain metastases, highlighting their significance in CNS oncology. We also discuss recent clinical trials investigating RAS-targeted therapies, including covalent inhibitors, MEK inhibitors, and novel combination approaches. Given the increasing recognition of RAS pathway alterations in CNS malignancies, further research is needed to elucidate their role in tumor biology and explore targeted therapeutic interventions. Full article

Good leptomeningeal collaterals (LMCs) after large vessel occlusion (LVO) extend the time window for endovascular therapy. The mechanisms regulating LMC activation are not fully understood. The aim of this study was to investigate the potential role of two vasoactive molecules endothelin-1 (ET-1)—a vasoconstrictor agent—and nitric oxide (NO)—a vasodilator agent—in the regulation of post-stroke LMCs. Ischemic stroke patients within 6 h of LVO were included. Collateral status was assessed using the Menon scoring system based on computed tomography angiography scans. Patients were accordingly divided into three groups: poor, intermediate, and good LMCs. Recanalization was evaluated using the modified thrombolysis in cerebral infarction (mTICI) score. Serum levels of ET-1 and NO were measured at three time points: T0 (<6 h), T1 (24 h), and T2 (48 h). A total of 105 patients were enrolled (mean age 76 ± 12.8 years): 44 with good (46.2%), 36 with intermediate (37.8%), and 22 with poor LMCs (23.1%). NO values decreased, whereas ET-1 values increased from T0 to T1 in all groups of patients. No significant association was found between serum ET-1 levels and collateral status. Higher ET-1 levels at T1 correlated with poor outcome regardless of the LMC status or the degree of recanalization (p = 0.030). A significant linear positive correlation was revealed at T0 between high levels of ET-1 and the neutrophil count (Spearman’s rho = 0.236, p = 0.035). Subgroup analysis showed a significant inverse correlation at T1 between NO and the collateral score (Spearman’s rho = −0.251, p = 0.021). Although we observed no significant association between LMC score and serum ET-1 concentrations, at 24 h higher ET-1 serum levels were predictive of poor outcome and higher NO levels were correlated with poor collateral status. These findings may indicate an inadequate microvascular reperfusion, possibly due to ET-1-mediated vasoconstriction, neutrophil activation, and NO-mediated oxidative stress, suggesting their potential role in the no-reflow phenomenon. Full article

This study introduces a novel system for evaluating hand motor function through synergy-based analysis during object manipulation in virtual and mixed-reality environments. Conventional assessments of hand function are often subjective, relying on visual observation by therapists or patient-reported outcomes. To address these limitations, we developed a system that utilizes the leap motion controller (LMC) to capture finger motion data without the constraints of glove-type devices. Spatial synergies were extracted using principal component analysis (PCA) and Varimax rotation, providing insights into finger motor coordination with the sparse decomposition. Additionally, we incorporated the HoloLens 2 to create a mixed-reality object manipulation task that enhances spatial awareness for the user, improving natural interaction with virtual objects. Our results demonstrate that synergy-based analysis allows for the systematic detection of hand movement abnormalities that are not captured through traditional task performance metrics. This system demonstrates promise in advancing rehabilitation by enabling more objective and detailed evaluations of finger motor function, facilitating personalized therapy, and potentially contributing to the early detection of motor impairments in the future. Full article