Search Results (41)

In cultured skin cells, decreases in antioxidant function and increases in intracellular free Fe²⁺ due to replicative aging have been reported. The Fenton reaction between Fe²⁺ and hydrogen peroxide is a threat to the skin because it produces hydroxyl radicals that attack proteins, nucleic acids and lipids. The purpose of this study was to determine whether exogenous iron modulation alters intracellular hydroxyl radicals in senescent normal human dermal fibroblasts (NHDFs). As previously reported, reduced antioxidant function, the accumulation of Fe²⁺ and increased levels of Reactive Oxygen Species (ROS) were observed in senescent NHDFs. The novel catalase (CAT) activity assay demonstrated a decrease in CAT activity alone in aged NHDFs. However, sufficient CAT activity against hydrogen peroxide was still maintained. Young NHDFs showed an increase in intracellular Fe²⁺ and hydroxyl radical signals after exogenous iron supplementation, both of which were cancelled by an iron chelator. Under the same experimental conditions, aged NHDFs that already showed a higher concentration of intracellular Fe²⁺ and stronger hydroxyl radical signals than young NHDFs also elicited a reduction in these levels after the addition of an iron chelator. These results suggest that exogenous regulation of intracellular iron concentration by iron chelators can suppress hydroxyl radical production independently of senescence progression, offering promise for future developments in senescence prevention research. Full article

Educators in America should be concerned about the failure of dialogue evident in television, newspapers, journals, and social media. Not only are people unable to talk and listen to each other, but some types of research are forbidden, stories are retracted in journals or newspapers because of a backlash, speakers are silenced on college campuses, and sometimes unfruitful conversations lead to violence. One is reminded of Raskolnikov’s dream in Dostoevsky’s Crime and Punishment, where the protagonist dreams of a plague that infects people with the idea that they alone have the truth, have the inability to understand each other, and end up destroying each other. The so-called “cancel culture” that we are suffering today is the fulfillment of this prophetic dream. It is rooted, not in modern technologies, but in the modern understanding of the person as an abstract, disengaged self that inevitably leads to empty conversations. What educators need today is to offer a richer metaphysics of the human person and an environment where students can learn what it means to grow in the truth together. In this paper, I will argue that (1) the failure of modern conversations is rooted in the flawed anthropology of modernity, (2) offer a richer metaphysics of the human person that can enable true dialogue, and (3) propose that Luigi Giussani’s Christian educational method can offer educators in the classroom a way to respond to cancel culture in a fruitful way. Full article

The Harry Potter fandom community around the world prefers a universe of wizards and witches that includes all people, but also has concerns about the author’s perspective regarding gender identity. This disjunction paralyzes the cultural reader with moral confusion, which is a danger to their emotional investment in the text. Although scholars have analyzed this phenomenon using fragmented prisms, such as social media activism, cognitive engagement, translation, pedagogy, and fan creativity, there is no unifying model that can be used to understand why reading pleasure endures. This article aims to fill this gap by examining these strands of research in a divergent manner, adopting a convergent mixed-methods study approach. Based on neurocognitive (EEG) values, cross-cultural focus groups, social media analysis, and corpus linguistics, we outline the terrain of reader coping mechanisms. We identify separate fan fractions and examine the corresponding practices. The results are summarized by proposing a model called the MDRL (Moral dissonance repair loop) which is a theoretical model that shows how translation smoothing, pedagogical reframing and fan-based re-moralization interact with one another in creating a system that enables the reader to be collectively able to obtain their relations with the text back to a manageable point and continue being engaged. This model makes a theoretical contribution to new areas in the study of fans, moral psychology, and cognitive literature. Full article

Biogenic hydroxyapatite is known for its osteoinductive potential due to its similarity to human bone and biocompatibility, but insufficient vascularization compared to autogenous bone during early implantation limits bone integration and osteogenesis. Fluorine has been shown to improve hydroxyapatite’s mechanical properties and the coupling of osteogenic and angiogenic cells. In this study, fluorine-modified biogenic hydroxyapatite (FPHA) with varying fluorine concentrations was prepared and tested for its ability to promote vascularized osteogenesis. FPHA prepared in this study retained the natural porous structure of biological cancellous bone and released F⁻ ions when immersed in cell culture medium. The extraction solutions of FPHA0.25 and FPHA0.50 promoted the formation of capillary-like tubes by human umbilical vein endothelial cells (HUVECs), with FPHA0.25 significantly upregulating vegf mRNA and VEGF protein levels in co-cultured human bone marrow mesenchymal stem cells (HBMSCs). Additionally, FPHA0.25 and FPHA0.50 upregulated pdgf-bb mRNA and PDGF-BB protein levels in HUVECs. In vivo experiments using a rabbit cranial defect model demonstrated that FPHA0.25 promoted early bone formation and angiogenesis in the defect area, enhanced VEGF secretion, and increased PDGFR-β expression in endothelial and mesenchymal cells. These findings suggest that fluorine-modified biogenic hydroxyapatite with an optimal fluorine concentration (FPHA0.25) may offer a promising strategy to enhance the body’s innate bone-healing potential by accelerating vascularization. Full article

Periodontal disease is considered one of the diabetic complications with high morbidity and severity. Recent studies demonstrated the involvement of the epigenome on diabetic complications. Histone modifications change chromatin architecture and gene activation. Histone modifications have been reported to alter chromatin structure and regulate gene transcription. In this study, we investigated the impacts of H3 lysine 4 trimethylation (H3K4me3) and specific histone methyltransferases of H3K4 methylation, su(var)3-9, enhancer-of-zeste, and trithorax domain 1A (SETD1A) on periodontal tissue affected by the diabetic condition. We observed the increase in H3K4me3 and SETD1A in gingival tissue of diabetic rats compared with the normal rats. Cultured human fibroblasts (hGFs) confirmed a high glucose-induced increase in H3K4me3 and SETD1A. We further demonstrated that high glucose increased the gene expression of matrix metalloproteinase (MMP) 1 and MMP13, which were canceled by sinefungin, an SETD1A inhibitor. Our investigation suggests that diabetes triggers histone modifications in the gingival tissue, resulting in gingival inflammation. Histone modifications may play crucial roles in the development of periodontal disease in diabetes. Full article

When analyzing road safety across cultural boundaries, driver behavior is a crucial component to consider. Given that driver behavior directly affects both the likelihood of accidents and the severity of their implications, it is crucial to comprehend and analyze it. The present study examined the differences in dangerous, aberrant, and positive driving behaviors across China and Pakistan. The effects of these behaviors on road traffic accidents were also considered. In the study, 1253 respondents completed a questionnaire package consisting of the Dula Dangerous Driving Index (DDDI), Aberrant Driving Behavior Questionnaire (DBQ), Positive Driving Behavior Scale (PDBS), and items related to demographics. Generalized linear models were utilized to compare and determine the factors responsible for dangerous driving behaviors. Mean scores for DBQ and PDBS items were compared. Finally, binary logistic regression models were used to find the factors responsible for traffic accidents across both countries. The results indicated that aggressive and risky driving predicted traffic accidents in both countries, followed by errors and violations, which also predicted traffic accidents significantly. Positive driving behaviors predicted accidents negatively in both samples. Furthermore, it was found that Chinese drivers compared to Pakistani drivers are less aggressive and risk-taking and commit fewer violations and errors while driving. To increase road safety in Pakistan, traffic laws must be strictly enforced uniformly, and violations must result in severe penalties, i.e., demerit points or cancellation of a driver’s license. Programs for road safety awareness and education must be expanded. Employing national culturally concentrated road safety strategies may be a more effective way to encourage safe driving behaviors. Full article

Patients treated for systemic urinary tract infections commonly have nonspecific presentations, and the specificity of the results of the urinalysis and urine cultures is low. In the following narrative review, we will describe the widespread misuse of urine testing, and consider how to limit testing, the disutility of urine cultures, and the use of antibiotics in hospitalized adult patients. Automated dipstick testing is more precise and sensitive than the microscopic urinalysis which will result in false negative test results if ordered to confirm a positive dipstick test result. There is evidence that canceling urine cultures if the dipstick is negative (negative leukocyte esterase, and nitrite) is safe and helps prevent the overuse of urine cultures. Because of the side effects of introducing a urine catheter, for patients who cannot provide a urine sample, empiric antibiotic treatment should be considered as an alternative to culturing the urine if a trial of withholding antibiotic therapy is not an option. Treatment options that will decrease both narrower and wider spectrum antibiotic use include a period of watching and waiting before antibiotic therapy and empiric treatment with antibiotics that have resistance rates > 10%. Further studies are warranted to show the option that maximizes patient comfort and safety. Full article

In 2023, the number of Chinese tourists visiting Malaysia had not yet returned to pre-pandemic levels, unlike those from some regions where tourism numbers have normalized. The lack of established research methodologies complicates the determination of whether negative news reports contribute to reduced visit intentions among these tourists. Through semi-structured interviews with a total of 69 individuals, including tourists who have visited, those who planned to visit but canceled, and local industry professionals, and using thematic analysis, this study identified ten primary factors diminishing Chinese tourists’ visit intentions regarding Malaysia. Notably, the findings suggest that the main reasons are not primarily associated with negative media coverage. The research indicates that improvements in multilingual services, targeted marketing strategies, effective use of Chinese social media platforms, promotion of local culture, addressing inaccuracies in religious and cultural guidance, and reducing regional disparities in infrastructure could enhance the visit intentions of Chinese tourists regarding Malaysia. This study not only offers a comprehensive framework for understanding the factors influencing visitation intentions but also provides an effective methodology for assessing the impact of unforeseen events on tourist behavior. It further proposes practical strategies to enhance the recovery of tourist arrivals. Full article

In the field of historical buildings, the wide use of lime as a binder in various contexts of application emerges from a series of artistic and archaeological evidence in the Apulia (Italy) from the 4th century BCE to the 15th century CE. The large availability of carbonate rocks in the geological substratum from Daunian Subappennines to Salento areas strongly influenced the material culture of the region. In this paper, significant study cases were presented to bring to light the technological complexity, almost completely cancelled by the widespread presence of industrial products, in the use of lime over the centuries. Through examples of use from antiquity to the modern age in Apulia (Egnatia, Lamapopoli, Tertiveri, Siponto, Lucera and Monopoli sites), technological solutions indicating an ecological dimension of production were discussed, bearing witness to technologies on a human scale and sustainability. The comparison of petrographical (POM, SEM-EDS) and mineralogical (XRPD) results indicated the technological trend and custom for lime production in the Apulian region that starts from the choice of the stone to be calcined and the aggregates and passes through the modalities of lime hydration and preparation of the mixture up to the laying. Full article

The Russian–Ukrainian conflict, in addition to causing an unacceptable loss of human life, is straining the integrity of Ukraine’s cultural heritage, despite the fact that both countries involved are parties to the 1954 Hague Convention for the Protection of Cultural Property in the Event of Armed Conflict and its First Protocol. Churches are one of Ukraine’s most important historical assets, as well as symbolic places of Orthodox religious identity common to both the invaders and the invaded. The destruction of these places and their deliberate damage on the part of both sides appear to be part of a more general conflict concerning internal disagreements between Russian and Ukrainian Orthodoxy, which, in turn, reflect two different historical views of the Russian–Ukrainian relationship. A brief reconstruction of relations between the Orthodox Churches operating on the territory of Ukraine demonstrates how religious affiliation has affected the conflict, causing it to become decisive and deeply divisive, so much so that the Patriarchate of Moscow has become an active part of the conflict. This circumstance favours the hypothesis that it is precisely the religious cultural heritage that is most at risk of deliberate destruction. The Russians, by destroying the symbolic places of Ukrainian religious identity (urbicide), affirm the spiritual unity of the Russian and Ukrainian peoples. For their part, the Ukrainians attempt to erase the Russian presence and the common religious cultural roots by destroying buildings of worship dear to the tradition of the Moscow Patriarchate (cancel culture). They reject the imperial traditions of Russia and, at the same time, claim an independent Church. The question arises as to whether the reconstruction process following the war will take into account the original cultural–religious identities, or whether it will take the opportunity to adopt a new (also) religious identity instead, and whether the old and new instruments offered by law are adequate. Full article

Sometimes, for medical reasons, when a frozen tissue has already thawed, an operation by re-transplantation may be cancelled, and ovarian tissues should be re-frozen for transplantation next time. Research about the repeated cryopreservation of ovarian cells is rarely reported. It has been published that there is no difference in the follicle densities, proportions of proliferation of early preantral follicles, appearance of atretic follicles, or ultrastructural quality of frozen-thawed and re-frozen-rethawed tissue. However, the molecular mechanisms of a repeated cryopreservation effect on the developmental potential of ovarian cells are unknown. The aim of our experiments was to investigate the effect of re-freezing and re-thawing ovarian tissue on gene expression, gene function annotation, and protein–protein interactions. The morphological and biological activity of primordial, primary, and secondary follicles, aimed at using these follicles for the formation of artificial ovaries, was also detected. Second-generation mRNA sequencing technology with a high throughput and accuracy was adopted to determine the different transcriptome profiles in the cells of four groups: one-time cryopreserved (frozen and thawed) cells (Group 1), two-time cryopreserved (re-frozen and re-thawed after first cryopreservation) cells (Group 2), one-time cryopreserved (frozen and thawed) and in vitro cultured cells (Group 3), and two times cryopreserved (re-frozen and re-thawed after first cryopreservation) and in vitro cultured cells (Group 4). Some minor changes in the primordial, primary, and secondary follicles in terms of the morphology and biological activity were detected, and finally, the availability of these follicles for the formation of artificial ovaries was explored. It was established that during cryopreservation, the CEBPB/CYP19A1 pathway may be involved in regulating estrogen activity and CD44 is crucial for the development of ovarian cells. An analysis of gene expression in cryopreserved ovarian cells indicates that two-time (repeated) cryopreservation does not significantly affect the developmental potential of these cells. For medical reasons, when ovarian tissue is thawed but cannot be transplanted, it can be immediately re-frozen again. Full article

The repair of orthopedic and maxillofacial defects in modern medicine currently relies heavily on the use of autograft, allograft, void fillers, or other structural material composites. This study examines the in vitro osteo regenerative potential of polycaprolactone (PCL) tissue scaffolding, fabricated via a three-dimensional (3D) additive manufacturing technology, i.e., a pneumatic micro extrusion (PME) process. The objectives of this study were: (i) To examine the innate osteoinductive and osteoconductive potential of 3D-printed PCL tissue scaffolding and (ii) To perform a direct in vitro comparison of 3D-printed PCL scaffolding with allograft Allowash^® cancellous bone cubes with regards to cell-scaffold interactions and biocompatibility with three primary human bone marrow (hBM) stem cell lines. This study specifically examined cell survival, cell integration, intra-scaffold cell proliferation, and differentiation of progenitor cells to investigate the potential of 3D-printed PCL scaffolds as an alternative to allograft bone material for the repair of orthopedic injuries. We found that mechanically robust PCL bone scaffolds can be fabricated via the PME process and the resulting material did not elicit detectable cytotoxicity. When the widely used osteogenic model SAOS-2 was cultured in PCL extract medium, no detectable effect was observed on cell viability or proliferation with multiple test groups showing viability ranges of 92.2% to 100% relative to a control group with a standard deviation of ±10%. In addition, we found that the honeycomb infill pattern of the 3D-printed PCL scaffold allowed for superior mesenchymal stem-cell integration, proliferation, and biomass increase. When healthy and active primary hBM cell lines, having documented in vitro growth rates with doubling times of 23.9, 24.67, and 30.94 h, were cultured directly into 3D-printed PCL scaffolds, impressive biomass increase values were observed. It was found that the PCL scaffolding material allowed for biomass increase values of 17.17%, 17.14%, and 18.18%, compared to values of 4.29% for allograph material cultured under identical parameters. It was also found that the honeycomb scaffold infill pattern was superior to the cubic and rectangular matrix structures, and provided a superior microenvironment for osteogenic and hematopoietic progenitor cell activity and auto-differentiation of primary hBM stem cells. Histological and immunohistochemical studies performed in this work confirmed the regenerative potential of PCL matrices in the orthopedic setting by displaying the integration, self-organization, and auto-differentiation of hBM progenitor cells within the matrix. Differentiation products including mineralization, self-organizing “proto-osteon” structures, and in vitro erythropoiesis were observed in conjunction with the documented expression of expected bone marrow differentiative markers including CD-99 (>70%), CD-71 (>60%), and CD-61 (>5%). All of the studies were conducted without the addition of any exogenous chemical or hormonal stimulation and exclusively utilized the abiotic and inert material polycaprolactone; setting this work apart from the vast majority of contemporary investigations into synthetic bone scaffold fabrication In summary, this study demonstrates the unique clinical potential of 3D-printed PCL scaffolds for stem cell expansion and incorporation into advanced microstructures created via PME manufacturing to generate a physiologically inert temporary bony defect graft with significant autograft features for enhanced end-stage healing. Full article

The COVID-19 pandemic has had a significant impact on numerous industries, including the event industry, resulting in widespread disruptions. The widespread cancellations of festivals have been a direct consequence of the pandemic, and, following the reopening, those that have taken place have had to implement changes in response to the new guidelines and regulations created as a result of the pandemic. In this study, we examine the experiences of festivals held in 2021 in the Veszprém–Balaton 2023 European Capital of Culture region (VEB 2023 region). The study aims to adapt the PwC Risk Management Model and identify its four pillars (1: detect; 2: protect; 3: react; 4: restore) in the case of festivals. Our study outlines how festival organizers have faced unprecedented challenges and risks (detect), how they survived in complete uncertainty (protect), what lessons they learned, and what risk management decisions they made as a challenge of the pandemic period from a consumer perspective (react). The present study utilized a qualitative research methodology and involved conducting structured interviews with a total of 19 event organizers from five different events. In addition, to gain a comprehensive understanding of the sustainable consumption habits of festival visitors, a questionnaire survey was administered yielding 1133 responses. The biggest challenges for the organizers during the times of the pandemic were uncertainty and unpredictability, with increased financial, human, and mental risks and burdens. However, the positive benefits of the pandemic period and the future developmental directions of sustainable festivals were also highlighted (e.g., small-scale, family-friendly events). Regarding the future, the aspects of greening aspirations, a need to reflect on the social-environmental criteria of sustainability, and more flexible management decisions to deal with uncertainty have emerged among the festival organizers (restore). The research has revealed that festival visitors themselves will be important allies in the introduction of sustainable measures. The research result helps festival organizers to adapt more effectively to the new socio-economic circumstances caused by the pandemic. Full article

The COVID-19 pandemic has resulted in significant changes in every aspect of our lives. Because of the measures imposed, people were only allowed to leave their homes for certain purposes, and all types of cultural and sports events were canceled. Climbers were greatly affected by these limited options for regular physical activity outside of the home environment. Little is known about the crisis’ effects on the climbing community in German-speaking regions. Thus, we surveyed 1028 German-speaking climbers (mean age 34.6 years, SD 10.4; 50.4% females) from December 2020 to February 2021. A cross-sectional online survey collected data on climbing frequency and preferences as well as levels of life satisfaction, using the standardized Short Life Satisfaction Questionnaire for Lockdowns (SLSQL) before and during the crisis. Results showed that due to the pandemic, study subjects climbed less frequently, preferred outdoor locations to climb, and showed decreased life satisfaction scores (21%, (d = 0.87, p < 0.001). In conclusion, these findings highlighted that the COVID-19 pandemic had a negative effect on climbing sports activities and life satisfaction in this study sample. To preserve physical and mental health, indoor and outdoor sport activities should be continued as much as possible with reasonable hygiene concepts in place. Full article

To elucidate the additive effects of the ROCK inhibitors (ROCK-i), ripasudil (Rip) and Y27632 on bimatoprost acid (BIM-A), a prostaglandin analog (PG), on adipose tissue, two- and three-dimensional (2D or 3D) cultures of 3T3-L1 cells, the most well characterized cells in the field of lipid research, were used. The cells were subjected to a variety of analyses including lipid staining, real-time cellular metabolic analysis, the mRNA expressions of genes related to adipogenesis and extracellular matrices (ECMs) as well as the sizes and physical properties of the 3D spheroids by a micro-squeezer. BIM-A induced strong inhibitory effects on most of the adipogenesis-related changes in the 2D and 3D cultured 3T3-L1 cells, including (1) the enlargement and softening of the 3D spheroids, (2) a dramatic enhancement in lipid staining and the expression of adipogenesis-related genes, and (3) a decrease in mitochondrial and glycolytic metabolic function. By adding ROCK-i to the BIM-A, most of these BIM-A-induced effects were cancelled. The collective findings reported herein suggest that ROCK-i eliminated the PG-induced suppression of adipogenesis in the 3T3-L1 cells, accompanied by the formation of enlarged 3D spheroids. Such effects of adding ROCK-i to a PG in preadipocytes on cellular properties appear to be associated with the suppression of PG-induced adverse effects, and provide additional insight into our understanding of lipid-related research. Full article