Search Results (2,387)

Growing concerns within the petrochemical industry regarding the security and sustainability of supply sources have prompted a search for alternative solutions. In this context, research focused on plant-based chemistry aims to develop molecules that can be transformed into new materials, biotechnological tools or industrial alternatives to fossil compounds. In addition, society and cosmetics companies are seeking to simplify formulations. Accordingly, we provide compounds that combine several functionalities, providing 2-in-1 or even 3-in-1 products. We present here a green hemi-synthetic strategy to obtain original polyfunctional derivatives of catechin by grafting fatty acid or fatty-alanine compounds. These bi-modular and tri-modular compounds exhibit surface activity and radical scavenging activity. They show significant antibacterial activity against E. coli as well. Full article

Background: Treosulfan combined with fludarabine (FluTreo) has emerged as a reduced-toxicity alternative to conventional myeloablative conditioning in allogeneic hematopoietic cell transplantation (allo-HCT) for acute myeloid leukemia (AML) and related myeloid malignancies. Purpose: This study evaluates the safety, engraftment kinetics, and long-term outcomes of the FluTreo FT14 regimen in a real-world adult cohort. Materials and Methods: We conducted a prospective cohort study of 186 consecutive adults (18–70 years) undergoing allo-HCT between January 2015 and December 2024. Eligible diagnoses included de novo or secondary AML, myelodysplastic syndrome, and myelofibrosis. All received peripheral blood stem cells from matched or mismatched unrelated donors, HLA-matched siblings, or haploidentical relatives. The FT14 protocol comprised fludarabine 150 mg/m² over five days and treosulfan 42 g/m² over three days, with rabbit antithymocyte globulin (5 mg/kg) for unrelated grafts. Primary endpoints were neutrophil and platelet engraftment, donor chimerism, incidence of acute and chronic graft-versus-host disease (GVHD), overall survival (OS), disease-free survival (DFS), relapse, and treatment-related mortality (TRM). Kaplan–Meier, Cox regression, and Fine and Gray models were applied. Results: Median age was 59 years; diagnoses included de novo AML (43%), secondary AML (16%), MDS (25%), and MF (16%). Neutrophil and platelet engraftment medians were 10 and 12 days, respectively. Full donor chimerism (≥99%) was achieved by day 31. Grade III conditioning-related toxicity occurred in 3.2% of cases. Five-year cumulative incidences of grade II–IV acute GVHD and moderate/severe chronic GVHD were 37.6% and 30.6%. Median follow-up was 16.3 months; relapse occurred in 25.3%. Five-year OS and DFS were 71% and 49% overall (75.8% and 59% in CR1), with TRM of 15.3%. Disease relapse and acute GVHD independently predicted inferior OS, and acute GVHD predicted TRM. Conclusions: The FluTreo FT14 regimen achieves rapid engraftment, universal high donor chimerism, low severe toxicity, and durable survival, supporting its use as a myeloablative, reduced-toxicity conditioning option in myeloid malignancies. Full article

The development of organic–inorganic hybrid materials bearing both acidic and basic functionalities is a challenging task, as it requires preserving the integrity and availability of two distinct active sites within a single porous network, such as silica. In this study, we report a synthetic approach that combines established grafting and co-condensation methods to achieve a controlled distribution of acidic and basic sites in mesoporous silica. Although each strategy is well known individually, their deliberate integration provides a distinctive pathway to tune the spatial arrangement and mutual preservation of the two functionalities. A comprehensive, multi-technique characterization approach, including in situ analyses and probe molecule adsorption, was used to monitor structural and chemical changes in both the organic and inorganic components. The results reveal that the coexistence of acidic and basic groups is highly sensitive to the synthetic conditions and activation treatments. In particular, basic sites are prone to protonation during the conversion of thiol groups into strong sulphonic acid sites, resulting in a partial loss of basic activity. The extent of this effect depends on the specific preparation route. In conclusion, the combined synthetic and characterization approach offers valuable insights into the nature, stability, and availability of the functional sites, guiding the rational design of advanced bifunctional hybrid materials. Full article

Background/Objectives: The use of dentin grafts in bone regeneration has gained increasing attention as an alternative to conventional grafting materials. Mesenchymal stem cells (MSCs), known for their osteogenic potential, have been combined with various biomaterials to enhance regenerative outcomes. This study aimed to evaluate the regenerative potential of dentin grafts and bovine-derived xenografts, with or without MSCs, in experimentally created bone defects in a rat model. Methods: A total of 25 male rats were randomly assigned to five groups: control, dentin graft, dentin graft and MSC, xenograft, and xenograft and MSC. Standardized 2-mm cortical defects were created bilaterally in the femoral shafts. Histological and immunohistochemical analyses were performed after a 90-day healing period. Statistical evaluation was carried out using the Kruskal–Wallis H test and Bonferroni-adjusted pairwise comparisons. Results: Complete healing was achieved in all groups without evidence of complications or inflammatory reactions. Immunohistochemical staining demonstrated no positive vascular endothelial growth factor (VEGF), collagen type I (COL1), or osteopontin (OPN) reactions in defect areas, consistent with complete maturation, although collagen type 3 (COL3) positivity was observed in residual xenograft material. Quantitative analysis showed that the dentin graft and MSC group achieved the highest degree of new bone formation (M = 92.88%, SD = 6.09), significantly greater than the control (p = 0.002) and xenograft groups (p = 0.013). Conclusions: Both dentin grafts and xenografts demonstrated enhanced bone defect healing when combined with MSCs. Nevertheless, dentin grafts in conjunction with MSCs yielded the most favorable regenerative outcomes, suggesting their clinical superiority over conventional xenografts. Full article

Xenograft bone, autologous bone grafts or allogeneic bones from a bone bank are used for bone augmentation, reconstruction or other purposes, when the volume, shape, and size of each jawbone defect require different bone materials. In the case of some bigger and locally advanced bone defects, the use of allogeneic bone can be suitable and used with great success if the wound and bone are especially carefully maintained; however, the healing period of each bone depends on good and stable wound closure followed by improved local antiseptic protocol. The individuality of each bone defect might also require additional prophylactic titanium plating in order to decrease the risk of possible mandibular fracture or to help improve bone stability, reduce bone mobility and possible inflammation or granulation tissue formation. Early radiological estimation of bone healing evaluation might be troublesome and not fully visible in radiological evaluation in the early stages of bone healing. On the other hand, possible bone inflammation, radiolucent defects, and granulation formation could be noted in cases of acute or long-lasting bone grafting material inflammation, bacterial contamination within the bone defect area, or the presence of fistula. The presented case describes a very good outcome from a dentigerous cyst removal with bone defect grafting and plating; however, because of wound dehiscence and allogeneic bone graft exposure, the patient required one additional procedure. Full article

Glass fiber (GF) reinforced unsaturated polyester resin (UP) composites are used in cured-in-place pipe (CIPP) rehabilitation technology of drainage systems due to their low cost and excellent force chemical properties. However, the weak interfacial compatibility between GF and the polymer matrix limits the stress transfer efficiency. Herein, a strategy of a polyhydric boehmite (AlOOH) layer coated on GF (GF-AlOOH) was developed for improving the mechanical properties of UP composites, and the enhancement effects of the coating process were analyzed. The AlOOH-modified GFs significantly improved the flexural and tensile strengths of the modified composites by 41.21% and 21.05%, respectively. Moreover, the enhancement mechanism was explored by analyzing the surface chemical structure of GF-AlOOHs. The nano-AlOOH was grafted on the GF surface by O=Al–OH. Meanwhile, the increase in the mechanical properties of UP/GF-AlOOH was mainly attributed to the combined effect of mechanical interlocking interaction, covalent bonding and hydrogen bonding, which improved the interfacial adhesion between GF and UP. In summary, this work provides effective guidance for achieving high-quality interfaces in GF composites and offers important insights into designing durable and cost-effective materials for CIPP rehabilitation and broader infrastructure applications. Full article

The sustainable conversion of biogenic waste into high-value materials presents a promising approach for addressing environmental and industrial challenges. This work reports an advancement into antioxidant-enriched phosphate minerals derived from green conversion of biogenic calcium carbonates of crustaceans. We demonstrate the effectiveness of Raman technology in controlling conversion using phosphoric acid treatment. The effects of reaction parameters—including acid stoichiometry, granular size distribution, and thermal treatment at 700 °C and 1200 °C—were systematically evaluated. Raman spectroscopy results validated by X-ray diffraction (XRD) and SEM-EDX analyses revealed mixed-phase minerals monetite, brushite, whitlockite or hydroxylapatite, respectively. Notably, reducing particle size enhanced conversion efficiency by increasing the reactive surface area, while the use of excess phosphoric acid facilitated conversion to monocalcium phosphate and promoted the degradation of the organic matrix. Thermal treatment further altered the product composition: heating at 700 °C produced a whitlockite-rich phase, whereas treatment at 1200 °C shifted the balance toward hydroxylapatite. The synthesized calcium phosphate compounds, including hydroxylapatite, monocalcium phosphate, whitlockite, and brushite, hold significant practical utility in biomedical applications (such as bone grafts and dental implants), agriculture, and industrial processing. Moreover, we have proven that by controlling the reaction parameters the final product composition can be tailored according to the specific needs. A greener approach yields brushite, monetite, or monocalcium phosphate, while a more energy-demanding process, including heating to 1200 °C, yields a high-purity hydroxylapatite. This research offers a sustainable analytical route for producing high-purity calcium phosphate materials from wasted biomaterials, contributing to both the bioeconomy as well as scientific innovation. Full article

The interfacial properties in carbon fiber (CF)-reinforced polymer composites are substantially limited by the chemically inactive and smooth CF surfaces. In this study, zeolitic imidazolate framework 90 (ZIF90) was chemically grafted onto CF surfaces via polyethyleneimine (PEI) as a coupling agent to construct a hierarchical reinforcement interface in CF/epoxy composite. The successful synthesis of CF grafted with PEI and ZIF90 (CF-PEI-ZIF90) was systematically characterized by Fourier-transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), thermogravimetric analysis (TGA), scanning electron microscopy (SEM), and X-ray diffraction (XRD). The incorporation of ZIF90 nanocrystals and PEI molecules into CF surfaces effectively improved interfacial adhesion through mechanical interlocking and chemical interactions, thereby optimizing stress transfer efficiency at the fiber–matrix interface and improving the interfacial properties of the composite. Additionally, the resultant CF-PEI-ZIF90/epoxy composite demonstrated significant mechanical enhancement, with the tensile and bending strengths increasing by 33.5% and 21.4%, respectively, compared to unmodified CF/epoxy composites. This work provides a novel strategy for enhancing the interfacial performance of CF composites by leveraging the unique properties of metal-organic frameworks, which is critical for advancing high-performance structural materials in aerospace and automotive applications. Full article

This study synthesizes three new composites: chitin-cross-linked poly(ortho-phenylenediamine)-grafted silicon dioxide (CT-PoPD-grafted SiO₂), chitosan-cross-linked PoPD-grafted SiO₂ (CS-PoP-grafted SiO₂), and guar-gum-cross-linked PoPD-grafted SiO₂ (GG-PoPD-grafted SiO₂). These biopolymer-based materials were developed as cost-effective, biocompatible adsorbents with increased surface area for removing Acid Red 1 AR1) and Crystal Violet (CV) dyes. Structural and morphological analyses through Fourier-transform infrared spectroscopy, X-ray diffraction, and X-ray photoelectron spectroscopy (XPS) confirmed their successful synthesis. Adsorption studies were conducted under various conditions, including adsorbent dosage, pH, temperature, and contact time. Among the composites, GG-PoPD-grafted SiO₂ demonstrated superior performance, achieving 99.1% and 95.6% removal of AR1 and CV, respectively. Kinetic analysis revealed a pseudo-second-order model, while thermodynamic results indicated a spontaneous and endothermic adsorption process. In conclusion, the GG-PoPD-grafted SiO₂ composite exhibits significant potential as an effective and sustainable material for wastewater treatment. Full article

The genus Diaporthe consists of saprobes, endophytes, and important plant pathogens. Members of this genus are widely distributed and have a broad host range, including grapevines. This study aimed to establish a baseline survey to assess the diversity of Diaporthe species infecting propagation material and to explore their dynamics in disease development. Initially, a survey was conducted in a nursery field, and isolations were carried out from 2-month-old symptomatic grafted vines of cv. Agiorgitiko grafted onto rootstock Richter 110. The initial molecular identification of the isolated mycobiome at the genus level was carried out by sequencing the universal internal transcribed spacer (ITS) locus, while subsequent species-level identification of the Diaporthe isolates was performed through phylogenetic approaches coupled with morphological characterization. Based on the combined analysis, five phylogenetically distinct Diaporthe spp. were identified in this study, taxonomically assigned to D. ampelina, D. eres, D. foeniculina, D. serafiniae, and D. novem. Pathogenicity trials demonstrated that the most aggressive species were D. ampelina followed by D. eres, while the remaining species were classified as opportunistic or weak pathogens of grapevine. Overall, accurate identification and monitoring of Diaporthe species involved in propagation material infections are important in order to develop species-specific effective management strategies in grapevine nurseries. Full article

Background and Objectives: Adequate peri-implant soft tissue dimensions are essential for health, hygiene, and esthetics. When ridge volume is sufficient, phenotype modification may avoid bone grafting. This case report describes a pedicled roll flap performed concurrently with single-stage implant placement after spontaneous socket healing, without bone substitute, and assesses soft-tissue stability with serial intraoral scans. Clinical case: A single-tooth edentulous site underwent prosthetically driven, fully guided implant placement. A modified roll flap with vertical and palatal incisions was prepared; the de-epithelialized crestal connective tissue was elevated and rolled into a buccal envelope to augment thickness. No graft material was used. A provisional crown conditioned the emergence profile. Follow-up included photographs, radiographs, and intraoral scan superimpositions at 2 weeks, 3–4 months, 8 months, and 14 months after implant treatment. Healing was uneventful. Buccal soft-tissue thickness increased, keratinized mucosa was preserved, and midfacial levels remained stable. Emergence profile and papillae integrated harmoniously. Crestal bone levels were stable radiographically. Digital scans corroborated soft-tissue thickness maintenance. No donor-site morbidity occurred. Conclusions: In healed sockets with adequate bone, a modified pedicled roll flap at implant placement can thicken the peri-implant phenotype and achieve stable esthetic integration without bone substitutes. Full article

Background: Maxillomandibular bone defects present a complex challenge in regenerative medicine due to anatomical and functional intricacies. Calcium phosphate (CP)-based biomaterials have emerged as promising bone graft substitutes due to their biocompatibility, osteoconductivity, and bioactivity. Aim: This Review highlights recent clinical and experimental advancements in CP-based biomaterials for maxillomandibular bone regeneration, bridging the gap from bench to bedside. Method: An in vitro, in vivo, and clinical literature review was conducted to evaluate the performance of CP ceramics, including hydroxyapatite (HA), tricalcium phosphate (TCP), biphasic ceramics, and novel composites with polymers, growth factors, and nanoparticles. Results: Calcium phosphate-based biomaterials demonstrate excellent bone regeneration potential, with Beta-tricalcium phosphate (β-TCP) and HA being the most widely utilized. Composite scaffolds and 3-dimensional (3D)-printed constructs show enhanced mechanical properties and biological integration. Clinical trials have confirmed the safety and efficacy of CP-based materials, yielding promising outcomes in osteoconduction and defect healing. However, limitations persist regarding mechanical strength and long-term degradation profiles. Conclusions: CP-based biomaterials offer significant clinical promise for maxillomandibular bone regeneration. Continued advancements in scaffold design and biofunctionalization are crucial for overcoming current limitations and fully realizing their therapeutic potential. Full article

Background: In recent years, indications for liver transplantation have expanded, while the age of transplant recipients has significantly increased due to improvements in perioperative management. As clinical manifestations of posttransplant complications vary and are often nonspecific, the identification of appropriate biomarkers is important for the assessment of graft quality and early recognition of potential complications following liver transplantation. Liver-type FABP (L-FABP) is a small cytoplasmic protein found abundantly in hepatocytes and is involved in the intracellular transport of long-chain fatty acids. Elevated serum levels have been detected in acute and chronic liver failure, kidney failure, and some malignancies. Materials and Methods: We conducted a prospective, single-center study from July 2023 to January 2025, including 29 adult patients who underwent deceased-donor transplantation. Three patients were excluded due to inadequate sample withdrawals. Serum L-FABP was measured preoperatively and on postoperative days 1, 3, 5, 7, and 14. Clinical, surgical, and biochemical data were collected and analyzed using non-parametric statistical tests. Results: L-FABP levels were significantly higher on POD 7 in recipients of grafts from donors ≥ 65 years (p = 0.035), with no corresponding changes in standard liver function markers. While no significant differences in L-FABP levels were found between patients with and without infectious biliary or vascular complications (all p > 0.05), we proved a strong negative correlation between intraoperative blood transfusion volume and L-FABP levels on POD 5 (ρ = −0.677, p < 0.001) and POD 7 (ρ = −0.455, p = 0.025). Conclusions: Our findings suggest that L-FABP holds promise as a biomarker for the early detection of subclinical hepatic graft cellular injury, which is not detected by means of conventional biomarkers for liver function. Full article

Ferulic acid (FA), together with its polymers and derivatives, has been attracting growing attention as a building block for advanced sustainable polymeric materials due to its renewable origin, intrinsic antioxidant activity, and potential for biodegradability. This review aims to provide a comprehensive overview of recent progress in the synthesis and functionalization of FA-based polymers, covering polymerization strategies, enzymatic modifications, and grafting approaches. The physicochemical characteristics of these materials are discussed, with particular emphasis on thermal stability, antioxidant performance, controlled release of active agents, and their impact on the mechanical and barrier properties of polymer matrices. Furthermore, key application domains—including biomedicine, food packaging, and environmental engineering—are examined, highlighting both the advantages and current limitations associated with FA utilization. Finally, perspectives are outlined regarding the necessity for further research to enhance bioavailability, stability, and synthetic efficiency, as well as the potential of FA-derived polymers in the development of next-generation, functional, and environmentally sustainable materials. Full article

Background/Objectives: This study aimed to compare clinical and functional outcomes of anterior cruciate ligament reconstruction (ACLR) using peroneus longus tendon (PLT) versus hamstring tendon (HT) autografts in matched cohorts. Materials and Methods: A retrospective matched cohort study with 1:1 nearest-neighbor matching without replacement on sex (exact), age (±3 years), and time to last follow-up (closest match) was performed on consecutive primary single-bundle ACLR cases. After exclusions and follow-up completion, 77 PLT patients were matched 1:1 with 82 HT patients based on age, sex, and follow-up. Outcomes included the International Knee Documentation Committee (IKDC) Subjective Knee Evaluation Form, Lysholm Knee Score, Tegner Activity Scale, Tampa Scale for Kinesiophobia, knee range of motion, manual strength testing, stability tests, limb circumferences, and the Single-Leg Hop test. Additionally, ankle-specific function in the PLT group was assessed using the AOFAS and FADI scores. Results: Graft diameter was larger with PLT (8.5 ± 0.6 mm) than with HT (8.1 ± 0.7 mm; p = 0.003). Both groups demonstrated significant improvement from the preoperative to the postoperative period on the IKDC, Lysholm, and Tegner scores (all p < 0.001). Between groups, postoperative IKDC and Lysholm scores were comparable (both p > 0.05), and Tegner scores did not differ significantly (p > 0.05). Knee flexion loss was less frequent (9.1% vs. 68.3%; p = 0.001), and knee flexion strength was higher (4.7 ± 0.5 vs. 4.0 ± 0.8; p = 0.001) in PLT. Stability tests, LSI, extension strength, and limb circumferences were similar (all p > 0.05). Saphenous nerve symptoms were less frequent with PLT (13.0% vs. 29.3%; p = 0.010). Ankle function in PLT was preserved (AOFAS 96.9 ± 7.9; FADI 97.4 ± 8.5) with mild eversion limitation in 3 patients (3.9%). Re-rupture rates were low and did not differ significantly between groups (p > 0.05). Infections occurred only after HT, yielding a between-group difference (p = 0.030). Conclusions: PLT autografts provide clinical and functional outcomes comparable to those of HT, with advantages including a larger graft diameter, better preservation of knee flexion function, fewer symptoms related to the saphenous nerve, and excellent ankle outcomes. PLT is a safe and effective alternative for primary ACLR. Full article