Search Results (3,579)

Work-related musculoskeletal disorders (WMSDs) and psychological stress remain major occupational health challenges among teaching professionals in adult education, yet their interconnected causes are often underexplored. This narrative review aims to identify multifactorial risk factors that contribute to these conditions and to propose a comprehensive framework that enhances understanding of teaching professionals’ well-being. A systematic synthesis of recent epidemiological and occupational health studies was conducted to analyse both immediate and underlying determinants across human, workplace, organisational, and socioeconomic dimensions. The findings reveal that more than two-thirds of teaching professionals experience WMSDs, particularly in the neck and lower back, while psychological stress affects over seventy percent globally. The combined effects of poor ergonomics, prolonged static postures, excessive workload, and limited organisational support contribute significantly to both physical and psychological strain. Broader contextual influences such as job insecurity, insufficient institutional resources, and societal undervaluation further intensify these risks. The review identifies a reciprocal relationship between physical discomfort and psychological distress, where each condition amplifies the other through behavioural and physiological mechanisms. The proposed integrative framework establishes a foundation for targeted interventions and evidence-based policy, promoting a shift toward holistic, system-oriented approaches to occupational health for teaching professionals in professional education settings. Full article

Our understanding of Ghrelin, an endogenous ligand of the growth hormone secretagogue receptor 1a (GHSR1a), has expanded from considering it to be a “hunger hormone” to a pleiotropic regulator of whole-body physiology. This review synthesizes the current advances spanning ghrelin biogenesis, signaling, and systems biology. Physiologically, preproghrelin processing and O-acylation by ghrelin O-acyltransferase (GOAT) generate acyl-ghrelin, a high-potency GHSR1a agonist; des-acyl ghrelin predominates in circulation and exerts context-dependent, GHSR1a-independent, or low-potency effects, while truncated “mini-ghrelins” can act as competitive antagonists. The emergence of synthetic ligands, agonists, antagonists, and reverse-agonists has provided the necessary tools to decipher GHSR1a activity. Recent cryo-EM structures of GHSR1a with peptide and small-molecule ligands reveal a bipartite binding pocket and provide a framework for biased signaling, constitutive activity, and receptor partner selectivity. Beyond the regulation of feeding and growth-hormone release, ghrelin modulates glucose homeostasis, gastric secretion and motility, cardiovascular tone, bone remodeling, renal hemodynamics, and innate immunity. Ghrelin broadly dampens pro-inflammatory responses and promotes reparative macrophage phenotypes. In the emerging scholarship on ghrelin’s activity in the central nervous system, ghrelin has been found to influence neuroprotection, stress reactivity, and sleep architecture, and has also been implicated in depression, Alzheimer’s disease, and substance-abuse disorders. Practical and transitional aspects are also highlighted in the literature: approaches for ghrelin stabilization; recent GHSR1a agonists/antagonists and inverse agonists findings; LEAP-2-based strategies; and emerging GOAT inhibitors. Together, structural insights and pathway selectivity position the ghrelin system as a druggable axis for the management of inflammatory diseases, neuropsychiatric and addiction conditions, and for obesity treatment in the post-GLP-1 receptor agonist era. Full article

The hypothalamus orchestrates systemic homeostasis by integrating neuroendocrine, autonomic, and immune–metabolic functions. In this review, we synthesize evidence that hypothalamic microglia act as dual hubs along a periphery–central–periphery axis across three domains: Peripheral effects: Microglia decode circulating cues and relay them to hypothalamic circuits, thereby modulating autonomic tone and endocrine pathways that impact immune–metabolic balance. Central mechanisms: Microglia sense and shape hypothalamic neural circuits via cytokine–neurotransmitter crosstalk, synaptic remodeling, and glial–neuronal signaling that tune neuroendocrine output. Translational links: Microglial states associate with biomarkers and clinical phenotypes in metabolic and inflammatory disorders, suggesting therapeutic entry points. Drawing on the literature from the last 20 years, we highlight convergent mechanisms, unresolved heterogeneity across models, and priorities for causal dissection and target validation. This framework clarifies how hypothalamic microglia coordinate local CNS processes with systemic physiology in health and disease. Full article

Recent breakthroughs in machine learning, artificial intelligence, and the emergence of large datasets have made the integration of eye tracking increasingly feasible not only in computing but also in many other disciplines to accelerate innovation and scientific discovery. These transformative changes often depend on intelligently analyzing and interpreting gaze data, which demand a substantial technical background. Overcoming these technical barriers has remained an obstacle to the broader adoption of eye tracking technologies in certain communities. In an effort to increase accessibility that potentially empowers a broader community of researchers and practitioners to leverage eye tracking, this paper presents an open-source software platform: Beach Environment for the Analytics of Human Gaze (BEACH-Gaze), designed to offer comprehensive descriptive and predictive analytical support. Firstly, BEACH-Gaze provides sequential gaze analytics through window segmentation in its data processing and analysis pipeline, which can be used to achieve simulations of real-time gaze-based systems. Secondly, it integrates a range of established machine learning models, allowing researchers from diverse disciplines to generate gaze-enabled predictions without advanced technical expertise. The overall goal is to simplify technical details and to aid the broader community interested in eye tracking research and applications in data interpretation, and to leverage knowledge gained from eye gaze in the development of machine intelligence. As such, we further demonstrate three use cases that apply descriptive and predictive gaze analytics to support individuals with autism spectrum disorder during technology-assisted exercises, to dynamically tailor visual cues for an individual user via physiologically adaptive visualizations, and to predict pilots’ performance in flight maneuvers to enhance aviation safety. Full article

DNA is continuously exposed to endogenous and exogenous factors that induce oxidative modifications leading to mutations and genomic instability. Oxidative DNA damage plays a dual role, contributing to physiological signaling at low levels while promoting mutagenesis, carcinogenesis and degenerative diseases when unpaired. Among various lesions, an oxidized base, such as 8-oxo-2′-deoxyguanosine (8-oxodG), is one of the major biomarkers of oxidative stress and genomic damage. Cells have evolved sophisticated repair processes, including base excision repair (BER), nucleotide excision repair (NER), and mismatch repair (MMR), to maintain genomic integrity. Dysregulation or polymorphism of these repair genes has been linked with cancer, neurologic, and cardiovascular disorders. This review discusses an overview of what is presently known concerning oxidative DNA damage and repair mechanisms, particularly emphasizing their molecular players, signaling routes, and human disease implications. It further refers to the latest advances in CRISPR-based technologies and multi-omics approaches that are redefining our understanding of DNA damage response (DDR) networks and creating new frontiers for therapeutic interventions. Full article

Huntingtin (HTT) is a large, ubiquitously expressed scaffold protein that participates in multiple cellular processes, including vesicular transport, transcriptional regulation, and energy metabolism. The mutant form of HTT (mHTT), characterized by an abnormal polyglutamine (polyQ) expansion in its N-terminal region, is the causative agent of Huntington’s disease (HD), a progressive neurodegenerative disorder. Current therapeutic efforts for HD have primarily focused on lowering HTT levels through gene silencing or promoting mHTT degradation. However, accumulating evidence suggests that post-translational modifications (PTMs) of HTT—such as phosphorylation, ubiquitination, acetylation, and SUMOylation—play pivotal roles in modulating HTT’s conformation, aggregation propensity, subcellular localization, and degradation pathways. These modifications regulate the balance between HTT’s physiological functions and pathological toxicity. Importantly, dysregulation of PTMs has been linked to mHTT accumulation and selective neuronal vulnerability, highlighting their relevance as potential therapeutic targets. A deeper understanding of how individual PTMs and their crosstalk regulate HTT homeostasis may not only provide mechanistic insights into HD pathogenesis but also uncover novel, more specific strategies for intervention. In this review, we summarize recent understanding on HTT PTMs, discuss their implications for disease modification, and outline critical knowledge gaps that remain to be addressed. Full article

Cardiovascular disorders and the medications used to treat them can affect physiological patterns of behavior. The aim of the present study was to determine whether the dual inhibition of neprilysin and angiotensin II—sacubitril/valsartan (ARNI) can modify anxiety-like behavior in male spontaneously hypertensive rats (SHR). We compared ARNI with two other drugs in the portfolio of heart failure treatment, captopril and ivabradine. Six groups (n = 13) of 12-week-old rats were treated for six weeks: control (Wistar rats), control + ARNI, SHR, SHR + ARNI, SHR + captopril, and SHR + ivabradine. The elevated plus maze test, the open field test, and the light–dark box test were used to determine anxiety-like behavior. SHRs exhibited higher systolic blood pressure (SBP), heart rate (HR), left ventricular weight (LVW), and hydroxyproline concentration (LVHP) but displayed a reduced level of anxiety-like behavior in comparison to controls. ARNI reduced SBP, HR, and LVW but had no significant effect on the level of anxiety in SHR, and similar results were achieved by captopril and ivabradine. Additionally, correlation analysis indicated that anxiety-like behavior in Wistar rats or SHR, either with or without cardiovascular therapy, was independent of SBP, HR, LVW, or LVHP. The level of anxiety-like behavior can, therefore, be considered part of the inherent neurobehavioral traits unrelated to fundamental hemodynamic or structural cardiovascular parameters. Full article

Transient Receptor Potential Vanilloid (TRPV) channels represent one of the seven subfamilies of TRP receptors and are widely expressed throughout the human body where they play pivotal roles in various physiological processes. In the gastrointestinal (GI) system, TRPV channels regulate critical functions such as nutrient absorption, motility, and secretions. Beyond maintaining cellular homeostasis, these channels are involved in pain and inflammation, contributing to diverse pathologies. Their central role in the pathophysiology of different digestive system disorders has made TRPV channels a significant focus of research. Moreover, the involvement of TRPV channels in numerous GI cancers has further heightened research interest in the role of these channels. Accordingly, this review elucidates the structural components and intricate signaling pathways of TRPV channels, focusing on the unique characteristics of each family member (TRPV1–6) in GI physiology. Furthermore, we explore the therapeutic potential of targeting these channels to modulate their physiological and pathological roles, highlighting their promise in treating GI disorders. Additionally, we address the challenges associated with their therapeutic application, considering their interactions in different systems, inherent biochemical characteristics, and the alterations required for effective design. Full article

Anxiety disorders are among the most prevalent mental health conditions worldwide, yet their assessment and treatment have long been limited by insufficient validity. To address this challenge, researchers have increasingly sought to translate approach–avoidance conflict paradigms from animal models into human experimental tasks. This review synthesizes the translational practices of four classic paradigms, namely the conditioned conflict paradigm, the open-field test, the Morris water maze, and the elevated plus maze, and introduces a “three-level, five-dimension” evaluation framework. The framework encompasses experimental design (reproducibility and operability), construct measurement (construct validity), and applied functionality (predictive and discriminant validity). Evaluation of existing studies indicates that human translational paradigms are generally feasible, showing strengths in operability and reproducibility. These paradigms reveal behavioral patterns consistent with animal anxiety models, underscoring their translational potential. However, evidence remains largely limited to behavioral indices, with little integration of subjective, physiological, or neural measures. Predictive validity is scarcely tested, and discriminant validity is confined to broad group differences rather than clinical subtypes. Current human translational paradigms provide a useful starting point but fall short of capturing the complexity of human anxiety. Future research should strengthen ecological validity, incorporate multimodal indicators, and expand testing in clinical populations to enhance predictive and discriminant validity. Such efforts are essential for advancing these paradigms toward dynamic tracking and individualized applications in both research and clinical contexts. Full article

Low-complexity domains (LCDs) are protein regions characterized by a simple amino acid composition and low sequence complexity, as they are typically composed of repeats or a limited set of a few amino acids. Historically dismissed as “garbage sequences”, these regions are now acknowledged as critical functional elements. This review systematically explores the structural characteristics, biological functions, pathological roles, and research methodologies associated with LCDs. Structurally, LCDs are marked by intrinsic disorder and conformational dynamics, with their amino acid composition (e.g., G/Y-rich, Q-rich, S/R-rich, P-rich) dictating structural tendencies (e.g., β-sheet formation, phase separation ability). Functionally, LCDs mediate protein–protein interactions, drive liquid–liquid phase separation (LLPS) to form biomolecular condensates, and play roles in signal transduction, transcriptional regulation, cytoskeletal organization, and nuclear pore transportation. Pathologically, LCD dysfunction—such as aberrant phase separation or aggregation—is implicated in neurodegenerative diseases (e.g., ALS, AD), cancer (e.g., Ewing sarcoma), and prion diseases. We also summarize the methodological advances in LCD research, including biochemical (CD, NMR), structural (cryo-EM, HDX-MS), cellular (fluorescence microscopy), and computational (MD simulations, AI prediction) approaches. Finally, we highlight current challenges (e.g., structural heterogeneity, causal ambiguity of phase separation) and future directions (e.g., single-molecule techniques, AI-driven LCD design, targeted therapies). This review provides a comprehensive perspective on LCDs, illuminating their pivotal roles in cellular physiology and disease, and offering insights for future research and therapeutic development. Full article

Background: Exposure to potentially traumatic events (PTEs) during childhood and adolescence is relatively common and may result in the development of post-traumatic stress disorder (PTSD). Recent studies have demonstrated the utility of the network approach for examining PTSD symptoms. However, to date, no systematic review has focused exclusively on network-analytic evidence from child and adolescent samples, who require developmental specific evidence to inform clinical practice. Therefore, the present review aimed to summarize network-analytic studies investigating PTSD symptoms among trauma-exposed youth. Methods: Guided by the PRISMA guidelines, a systematic search for network-analytic studies exploring the symptom structure of PTSD-only in children and adolescents was conducted using PubMed and EBSCOHost. The methodological quality of included studies was assessed using the NIH Quality Assessment Tool for Observational Cohort and Cross-Sectional Studies. Results: Twelve studies (n = 12498; k = 18) were retrieved, with seven rated as of fair quality. Studies examined DSM-IV (n = 10) and DSM-5 (n = 2) PTSD symptoms in children and/or adolescents exposed to PTEs (mostly natural disasters). Although central symptoms varied, heightened physiological reactivity consistently emerged among the most central. The strongest associations were observed between symptoms within the same PTSD cluster, and preliminary evidence suggests that network connectivity may increase with time since exposure. Conclusions: Heightened physiological reactivity to trauma-related cues appears to be a clinically relevant feature of youth exposed to PTEs, warranting consideration in assessment and intervention. Clinical and methodological implications and future directions for pediatric PTSD research are discussed. Full article

The brainstem plays a pivotal role in the generation and control of eye movements—including saccades, smooth pursuit, the vestibulo-ocular reflex (VOR), vergence, and gaze holding. Beyond its vital physiological functions, it is also essential for the coordination of balance and movement. Consequently, eye movement disorders of brainstem origin are often accompanied by vertigo, imbalance, unsteady gait, and diplopia, particularly during changes in head or body position. A sound understanding of the neural structures involved in oculomotor and vestibular control is therefore crucial for accurately identifying and localizing a wide variety of brainstem syndromes. However, oculomotor abnormalities resulting from brainstem disease represent a major diagnostic challenge for the neurotologist, owing to the wide spectrum of possible etiologies (vascular, traumatic, degenerative, neoplastic), their variable severity and clinical course (acute, fluctuating, or progressive), and the frequent concomitant involvement of other central structures, particularly the cerebellum. This mini review summarizes the pathophysiological mechanisms and clinical features of oculomotor disorders and nystagmus associated with brainstem disease. Full article

Nephrotic syndrome (NS) is a systemic disorder characterized not only by glomerular dysfunction but also by profound dysregulation of lipid metabolism and microvascular integrity. Adipose tissue, as a central lipid-handling and endocrine organ, undergoes structural and functional remodeling in chronic renal conditions yet remains underexplored in this context. The aim of this manuscript is to integrate adipose tissue imaging into the diagnostic and mechanistic framework of NS. To establish this perspective, we first summarize current knowledge on adipose tissue architecture and imaging in both physiological states and renal disease. We then present a multimodal imaging approach—combining ultrasound (US), histology, and atomic force microscopy (AFM)—applied to human adipose tissue as a potential diagnostic and pathophysiological marker in NS. Original imaging from our laboratory experience is presented as a demonstrative material, complemented by literature synthesis. Given that different modalities of imaging-based characterization of adipose tissue are sparse across the literature, this pictorial review offers a guide to identifying structural biomarkers of adipose remodeling in NS. By bridging imaging modalities with metabolic and vascular perturbations observed in NS, this work aims to guide future research toward the clinical application of adipose tissue imaging in renal disease. This provides insights into cell size heterogeneity, vascular topology, and subcellular features such as membrane wrinkles and nanodomain organization. We propose that such morphometric parameters, accessible via minimally invasive biopsies, could serve as surrogate markers of adipose remodeling in nephrotic syndrome. This sets the stage for integrating adipose tissue imaging into the diagnostic and mechanistic evaluation of systemic features in NS. Full article

Background/Objectives: Eating disorders (EDs) and body image disturbances are increasingly recognized as important health issues among young athletes. Sports participation may both support healthy development and simultaneously increase vulnerability to disordered eating due to performance pressures and cultural ideals. The aim of this study was to assess the risk of eating disorders and body image among 17–20-year-old athletes. Methods: The study included 428 participants (215 women and 213 men) actively engaged in sports. Standardized psychometric tools were applied, including the Eating Attitudes Test (EAT-26) and the Body Esteem Scale (BES). Statistical analyses examined differences across gender, BMI categories, and sports disciplines, as well as predictors of ED risk. Results: The analysis showed that 32.9% (n = 141; 95% CI: 28.3–37.8%) of respondents were at risk of developing eating disorders, with women being significantly more vulnerable than men (p < 0.001; V = 0.27). Underweight athletes demonstrated a higher risk compared with those of normal weight (OR = 2.86, 95% CI: 1.48–5.55, p < 0.001). The type of sport was also associated with risk (p < 0.001, V = 0.323); the highest prevalence of ED risk occurred among dancers (48.1%) and swimmers (38.9%). Body esteem differed markedly between groups: participants at risk scored lower in Weight Control (p < 0.001, Cohen’s d = 0.94) and Physical Attractiveness (p = 0.072) but higher in Physical Condition (p < 0.001). Regression analyses indicated that gender (β = −3.35, p < 0.001) and Body Esteem—Weight Control (β = −0.45, p < 0.001) were the strongest predictors of EAT-26 scores. Conclusions: The findings confirm the multidimensional nature of eating disorder risk among young athletes, highlighting the role of body image imbalance and gender differences. Early screening, preventive interventions, and multidisciplinary support are essential to protect both the physical and mental health of young athletes. Future research should include objective physiological measures and broader samples to improve generalizability. Full article

Background/Objectives: The aim of the study was to assess the emotional state (stress, mood, and anxiety level, including labor anxiety) of pregnant women depending on the course of pregnancy and the related place of stay (hospital pregnancy pathology department, home). Methods: A total of 100 participants were recruited between 25 and 38 weeks of pregnancy. A total of 88 fully completed questionnaires of women qualified for analysis, including 45 women staying in the hospital (G1) and 43 women who did not require hospitalization (G2). The Depression Anxiety Stress Scale (DASS-42), the Labor Anxiety Questionnaire (KLP II), the Fatigue Assessment Scale (FAS), and a self-administered questionnaire were used. Results: All subjects showed an average moderate level of depression and stress and a high level of anxiety. A statistically significant difference in mood level (DASS depression) was noted between group G1 and group G2 (p = 0.0217). About 35% of all subjects in total and both groups achieved a result indicating a severe or extremely severe level of stress. About 66% of subjects in both groups showed a severe and extremely severe level of anxiety. None of the women studied had values interpreted as a physiological level of anxiety. Conclusions: Regardless of the course of pregnancy and the related place of residence, the risk of emotional disorders is high. It seems reasonable to perform screening tests on pregnant women to identify those who may or already have these problems. Full article