Search Results (250)

Antimony (Sb), a toxic metalloid, inhibits plant growth and threatens human health. Yellow Stripe-Like (YSL) proteins play crucial roles in metal ion transport and cellular homeostasis. While the OPT gene family has been characterized in some species, its genome-wide organization and functional involvement in Sb stress response remain unexplored in Brassica juncea. Here, we identified 47 high-confidence BjOPT genes and combined transcriptomic approaches to elucidate their regulatory roles under Sb stress. Phylogenetic tree, conserved motifs, and gene structure analyses consistently distinguished the BjOPT and BjYSL subfamilies. Comparative and collinearity analyses indicated that OPT genes in Brassica species (including B. rapa, B. nigra, and B. juncea) expanded independently of whole-genome triplication events. Transcriptomic profiling revealed significant enrichment of differentially expressed genes (DEGs) related to key biological processes (oxidative and toxic stress response, metal ion transport, and auxin efflux) and pathways (glutathione metabolism, MAPK signaling, and phytohormone transduction), highlighting their roles in Sb detoxification and tolerance. Notably, three BjYSL3 (BjA10.YSL3, BjB02.YSL3, and BjB05.YSL3) genes exhibited strong up-regulation under Sb stress. Heterologous expression in yeast demonstrated that both BjA10.YSL3 and BjB02.YSL3 enhance Sb tolerance, suggesting their potential role in transporting Sb–nicotianamine (NA) or phytosiderophore (PS) complexes. These findings advance our understanding of Sb tolerance mechanisms and provide a basis for developing metal-resistant crops and phytoremediation strategies. Full article

Salicornia europaea is an extremely salt-tolerant annual halophyte. It occurs in coastal and inland saline habitats and is increasingly cultivated for food, nutraceuticals, and environmental remediation. This study examined whether inland populations from contrasting saline sites exhibit heritable differences in shoot and root morphology. Seeds from four isolated sites (Ciechocinek, Inowrocław, Salzgraben, and Soltauquelle) were grown at 0, 200, 400, and 1000 mM NaCl, and morphometric traits were quantified from digital images. Corresponding soil samples were also analyzed. A strong relationship was found between population origin and responses to salt stress. Optimal growth generally occurred at 200–400 mM NaCl. Shoot canopy area consistently best discriminated among populations. Inowrocław and Salzgraben performed best under extreme salinity (1000 mM), whereas Ciechocinek showed the weakest growth. Root analyses revealed a shift from radial expansion at moderate salinity to elongation at higher levels, with Salzgraben and Soltauquelle maintaining the longest roots. Soil analyses indicated substantial site differences: Ciechocinek had the highest salinity, resulting in the smallest trait development, Inowrocław was rich in Ca²⁺ and organic matter, while the German sites had lower salinity but higher pH and bicarbonates. These findings demonstrate population-specific adaptive strategies and soil-legacy effects, supporting targeted ecotype selection for saline agriculture and phytoremediation. Full article

The uptake and accumulation of nanoplastics by plants have emerged as a major research focus. Exogenous selenium nanoparticles (SeNPs) are widely used to mitigate the toxicity of abiotic stresses, such as nanoplastics (NPs) and polyethylene (PE—NPs) nanoplastics, and represent a feasible strategy to enhance plant performance. However, the molecular mechanisms by which SeNPs alleviate the phytotoxicity of microplastics and nanoplastics remain poorly defined. To address this gap, we used Pistia stratiotes L. (P. stratiotes) as a model and silicon dioxide nanoparticles (SiO₂NPs) as a comparator, integrating physiological assays, ultrastructural observations, and proteomic analyses. We found that NP stress caused ultrastructural damage in root tips, exacerbated oxidative stress, and intensified membrane lipid peroxidation. SeNPs treatment significantly mitigated NP-induced oxidative injury and metabolic suppression. Compared to the NPs group, SeNPs increased T-AOC by 38.2% while reducing MDA and ·OH by 33.3% and 89.6%, respectively. Antioxidant enzymes were also elevated, with CAT and POD rising by 47.1% and 39.2%. SeNPs further enhanced the photosynthetic capacity and osmotic adjustment, reflected by increases in chlorophyll a, chlorophyll b, and soluble sugar by 49.7%, 43.8%, and 27.0%, respectively. In contrast, proline decreased by 17.4%, indicating stress alleviation rather than an osmotic compensation response. Overall, SeNPs outperformed SiO₂NPs. These results indicate that SeNPs broadly strengthen anti-oxidative defenses and metabolic regulation in P. stratiotes, effectively alleviating NP-induced oxidative damage. Proteomics further showed that SeNPs specifically activated the MAPK signaling cascade, phenylpropanoid biosynthesis, and energy metabolic pathways, enhancing cell-wall lignification to improve the mechanical barrier and limiting NPs translocation via a phytochelatin-mediated vacuolar sequestration mechanism. SiO₂NPs produced similar but weaker alleviative effects. Collectively, these findings elucidate the molecular basis by which SeNPs mitigate NPs’ phytotoxicity and provide a theoretical foundation and practical outlook for using nanomaterials to enhance phytoremediation in aquatic systems. Full article

The growing global demand for mined commodities has intensified the environmental challenges associated with mine tailings. Currently, an estimated 62,381 mining properties impact approximately 50 million square kilometers of the Earth’s land surface. Annual tailings production exceeds 10 billion tonnes and is projected to reach 19 billion tonnes by 2025. This review examines phytoremediation strategies and the associated legal framework in South Africa, highlighting a critical disconnect between existing remediation approaches, environmental legislation, and the broader sustainable development agenda. To address these gaps, a fundamental shift towards a circular economy paradigm is essential—one that aligns research, policy, and practice to foster innovative, sustainable solutions. Phytoremediation using bioenergy crops such as Vetiver grass (Chrysopogon zizanioides) offers a holistic approach that integrates environmental restoration with circularity and economic viability, while avoiding competition with food crops for arable land. Full article

The use of high biomass production plants in studies of metal phytoremediation is an established practice. This strategy aims to identify plants that tolerate unusual amounts of metals such as nickel (Ni). When comparing the biomass production capacity of a Ni hyperaccumulator, such as plants from the Alyssum genus, a production ranging from 0.5 to 4 t ha⁻¹ per crop cycle is observed ; on the other hand, species with a high biomass production capacity, for example Canavalia ensiformis, can produce 20 t ha⁻¹ to 25 t ha⁻¹ of green phytomass, 5 t ha⁻¹ to 8 t ha⁻¹ of dry phytomass and 1000 kg ha⁻¹ to 1800 kg ha⁻¹ of seeds. In this context, we planned an experiment to verify the tolerance and Ni accumulation capacity in Canavalia ensiformis. Our hypothesis was that increasing Ni concentration in the soil would not hinder the plant’s biomass production. We conducted a completely randomized experiment with five concentrations of Ni added to the soil and five replicates in a greenhouse during the vegetative stage. We evaluated the plant’s development, biomass production, and Ni accumulation in its organs. Our results demonstrated high tolerance to the metal, maintaining a biomass accumulation capacity of 68% of the dry mass in the soil with 277.8 mg kg⁻¹ of Ni at the highest concentration tested, compared to plants in the control soil. Considering that under these conditions the plants obtained a biomass of 10 g of leaves and 15 g of roots, and a nickel accumulation capacity of 75.05 mg kg⁻¹ in leaves and 102 mg kg⁻¹ in roots, the total Ni accumulation in the plants reached 2.37 mg Ni/plant in the soil with 277.8 mg kg⁻¹ of Ni. This soil Ni concentration would be lethal for most plants, and the metal concentration in the tissue exceeds the established limits for non-tolerant crops. With these results, this study aims to provide a foundation for improving the use of Canavalia ensiformis in phytoremediation. Full article

The remediation of soils contaminated with heavy metals and radionuclides remains a significant environmental challenge. This study evaluated the phytoremediation potential of industrial hemp (Cannabis sativa L.) in soil collected from a historical evaporation dam, characterized by high levels of diverse metals, including Al, Cr, Fe, and radioactive elements (U, Th). Three treatments were applied: a control, a metal-spiked treatment (chelated with citric acid), and an NPK + spike treatment. A separate six-month greenhouse trial compared plants grown with and without NPK nutrients. Results demonstrated that the addition of a chelating agent significantly enhanced the bioavailability and subsequent uptake of key metals, including U, Se, and Pd. NPK fertilization combined with chelation resulted in the greatest plant biomass (≈4.5 g) and height (>18 cm), which correlated with higher total metal accumulation. Bioaccumulation factors (BAF > 1) were highest for B, Sr, Cd, and Bi, with values for Cd and U reaching 1.3 and 2.1, respectively. Foliar analysis revealed that leaves accumulated significantly higher metal concentrations than stems (e.g., Translocation Factor (TF) ~ 2.0 for Cd, Pb, and U), acting as the primary sink. This study concludes that hemp, particularly when assisted with chelating agents and adequate nutrition, is a highly effective candidate for the phytoremediation of multi-metal contaminated soils. The NPK + chelation strategy is the most promising for maximizing both biomass production and metal extraction efficiency. Full article

Cadmium (Cd) contamination in agricultural soils originates mainly from atmospheric deposition, irrigation water, fertilizers, pesticides, and industrial waste discharges. This human-induced pollution adversely affects soil fertility and structure, disrupts plant growth and physiological activities, and poses severe health risks through food-chain accumulation. Despite increasing research attention, comprehensive assessments that integrate global patterns, remediation strategies, and knowledge gaps remain limited. Therefore, this literature review critically synthesizes findings from 1060 peer-reviewed studies (screened using PRISMA guidelines) retrieved from Scopus and Web of Science databases, focusing on Cd sources, environmental behavior, plant responses, and soil remediation techniques. Results show that most research has been concentrated in Asia—particularly China—and Latin America. The most frequently investigated topics include Cd accumulation in crops, soil amendments, phytoremediation, and microbial-assisted remediation. Among remediation strategies, assisted phytoremediation and integrated biological–chemical approaches (biochar, PGPR, and soil amendments) emerged as the most promising for sustainable Cd mitigation. In conclusion, this review highlights regional disparities in research coverage, emphasizes the effectiveness of combined remediation approaches, and identifies the need for interdisciplinary and field-scale studies to advance sustainable solutions for Cd pollution control in agricultural systems. Full article

The alarming increase in the use of chemically driven pesticides for enhanced crop productivity has severely affected soil fertility, ecosystem balance, and consumer health. Inadequate handling protocols and ineffective remediation strategies have led to elevated pesticide concentrations, contributing to human respiratory and metabolic disorders in humans. In the current context, where agricultural activities and pesticide applications are intertwined, strong and sustainable remediation strategies are essential for environmental protection without sacrificing crop productivity. Various bio-inspired methods have been reported, such as phytoremediation, bioremediation, and in situ remediation; however, limited success has been observed with either single or combined approaches. Consequently, biopolymer biomanufacturing, nanoparticle-based bioengineering, and computational biology for improved understanding of mechanisms have been revisited to incorporate updated methodologies that detail the fate and action of harmful chemical pesticides in agriculture. An in silico mechanistic approach has been emphasized to understand the molecular mechanisms involved in agricultural pesticides’ degradation using nanomaterials. A roadmap has been created by integrating cutting-edge machine learning techniques to develop nature-inspired sustainable agricultural practices and contaminant disposal methods. This review represents a pioneering effort to explore the roles of wet-lab chemistry and in silico methods in mitigating the effects of agricultural pesticides, providing a comprehensive strategy for balancing environmental sustainability and agricultural practices. Full article

Lead (Pb) is a widespread environmental pollutant that severely threatens plant growth and development. While the mechanisms of Pb uptake and accumulation have been extensively studied in herbaceous plants, the glutathione (GSH)-mediated biochemical responses in woody species remain largely unexplored. This knowledge gap limits our understanding of the detoxification strategies of perennial plants with high phytoremediation potential. In this study, two Salix integra clones (P336 and P646) with contrasting Pb tolerance were used to investigate the temporal regulation of GSH metabolism under Pb stress. P336 displayed both early and sustained increases in cysteine (Cys), GSH, ascorbic acid (AsA), phytochelatins (PCs), and the activities of γ-ECS and APX, conferring stronger antioxidant and detoxification capacity than P646. Notably, glutathione reductase (GR) activity remained unchanged in both clones, indicating that GSH homeostasis was maintained mainly through de novo synthesis rather than GR-mediated recycling. These findings demonstrate that Pb tolerance in P336 is achieved through γ-ECS–driven de novo GSH biosynthesis, which sustains both the AsA–GSH cycle and PC synthesis for efficient ROS detoxification and Pb sequestration. By providing the first detailed evidence of GSH-centered detoxification dynamics in a woody phytoremediant, this study advances our mechanistic understanding of Pb tolerance in S. integra and highlights its application potential in the phytoremediation of Pb-contaminated environments. Full article

Environmental dissemination of antibiotics is a pressing global challenge, driving ecological imbalances and the proliferation of antibiotic resistance genes (ARGs). Conventional treatment technologies often fail to fully eliminate these micropollutants or are cost-prohibitive for widespread use. In this context, phytoremediation—using plants and their associated microbiota to remove, transform, or immobilize contaminants—has emerged as an effective and promising, low-impact, and nature-based approach for mitigating antibiotic pollution in aquatic and terrestrial environments. This review provides a comprehensive synthesis of the physiological, biochemical, and ecological mechanisms by which plants interact with antibiotics, including phytoextraction, phytodegradation, rhizodegradation, and phytostabilization. This review prioritizes phytoremediation goals, with attention to high-performing aquatic (e.g., Lemna minor, Eichhornia crassipes, Phragmites australis) and terrestrial plants (e.g., Brassica juncea, Zea mays) and their ability to remediate major classes of antibiotics. This study highlights the role of rhizosphere microbes and engineered systems in phytoremediation, while noting challenges such as variable efficiency, phytotoxicity risks, limited knowledge of by-products, and environmental concerns with antibiotic degradation. Future perspectives include the integration of genetic engineering, microbiome optimization, and smart monitoring technologies to enhance system performance and scalability. Plant-based solutions thus represent a vital component of next-generation remediation strategies aimed at reducing antibiotic burdens in the environment and curbing the rise in antimicrobial resistance. Full article

The extensive use of antibiotics in animal husbandry leads to the release of unmetabolised residues and the dissemination of antimicrobial resistance genes (ARGs) in manure, posing environmental and public health challenges. Conventional treatment technologies, including hydrolysis, photodegradation, and phytoremediation, are often constrained by incomplete mineralisation, high cost, and environmental variability. Biocatalytic and microbially mediated processes are increasingly recognised as sustainable alternatives. Enzymes, which in clinical contexts confer resistance, can, in environmental matrices, catalyse the dismantling of antibiotic scaffolds, attenuating bioactivity and promoting detoxification. Catalytic classes such as hydrolases, transferases, and oxidoreductases mediate diverse transformations, including hydrolytic cleavage, functional group transfer, and oxidative modification. Microbial consortia and bioaugmentation further enhance biodegradation, while biochar and other amendments reduce ARG persistence. Advances in multi-omics, enzyme engineering, and immobilisation have expanded catalytic repertoires, improved stability, and enabled integration with composting, anaerobic digestion, and hybrid bioprocesses. Nonetheless, incomplete degradation, recalcitrant intermediates, and horizontal gene transfer remain challenges. Importantly, since degradation products may leach into soils and aquatic systems, optimising these processes is critical to prevent residues from entering the water cycle. This review synthesises advances in microbial and enzymatic degradation strategies, highlighting opportunities for sustainable manure management while mitigating water pollution risks. Full article

This study presents a comprehensive bibliometric analysis of 217 publications on nanomaterials for soil and groundwater remediation, sourced from the Scopus database, covering the period from 2010 to 2024. The findings highlight significant contributions from various countries, with India identified as the leading contributor, followed by China and the United States. This reflects robust international collaboration in addressing environmental contamination. The analysis also identifies influential journals in this field, particularly “Science of the Total Environment” and “Environmental Science and Technology”, which are recognized for their high citation impact and play a crucial role in disseminating research findings and advancing knowledge in nanomaterials for environmental remediation. A keyword co-occurrence analysis reveals six distinct clusters that emphasize critical research themes. The first cluster focuses on environmental toxicity, underscoring the risks posed by contaminants, particularly heavy metals and emerging pollutants such as PFAS, highlighting the need for advanced monitoring strategies. The second cluster showcases innovative nanoremediation technologies, particularly zero-valent iron (nZVI) and carbon nanotubes (CNTs), which are noted for their effectiveness in pollutant removal despite challenges like surface passivation and high production costs. The third cluster addresses heavy metals and phytoremediation, advocating integrated strategies that enhance crop resilience while managing soil contamination. The fourth cluster explores photocatalysis and advanced oxidation processes, demonstrating how nanomaterials can enhance pollutant degradation through light-activated catalytic methods. The fifth cluster emphasizes adsorption mechanisms for specific contaminants, such as arsenic and pharmaceuticals, suggesting targeted remediation strategies. Finally, the sixth cluster highlights the potential of nanomaterials in agriculture, focusing on their role in improving soil fertility and supporting plant growth. Overall, while nanomaterials demonstrate significant potential for effective environmental remediation, they also pose risks that necessitate careful consideration and further research. Future studies should prioritize optimizing these materials for practical applications, addressing both environmental health and agricultural productivity. Full article

Soil lead (Pb) contamination poses a severe threat to agricultural sustainability and food security. Phytoremediation offers a green alternative for remediation, yet its efficiency is limited by poor plant tolerance and restricted metal uptake. In this study we investigated the functional roles of the microbial inoculants Trichoderma guizhouense NJAU4742 and Bacillus velezensis SQR9 in enhancing the performance of Salix suchowensis P1024 grown in Pb-contaminated soil. NJAU4742 significantly increased plant biomass by 34% (p < 0.05), accompanied by increased soil microbial biomass and higher activities of urease, acid phosphatase, and sucrase. In contrast, SQR9 strongly enhanced Pb accumulation by 19% (p < 0.05), which was accompanied by upregulated antioxidant enzymes, reduced lipid peroxidation, and elevated cysteine levels. Random forest and correlation analyses demonstrated that soil nutrient cycling indices (urease, MBC, sucrase) were key predictors of biomass, whereas antioxidant defenses (POD, CAT) primarily explained Pb accumulation. These findings provide new insights into the distinct contributions of NJAU4742 and SQR9 to willow growth and Pb remediation, and provide a basis for developing more effective microbe-assisted phytoremediation strategies. Full article

Cadmium (Cd), a pervasive and highly phytotoxic metal pollutant, poses severe threats to agricultural productivity, ecosystem stability, and human health through its entry into the food chain. Plants have evolved intricate defense mechanisms, among which the strategic manipulation of nutrient elements emerges as a critical physiological and biochemical strategy for mitigating Cd stress. This comprehensive review delves deeply into the multifaceted roles of essential macronutrient elements (nitrogen, phosphorus, potassium, calcium, magnesium, sulfur), essential micronutrient elements (zinc, iron, manganese, copper) and non-essential beneficial elements (silicon, selenium) in modulating plant responses to Cd toxicity. We meticulously dissect the physiological, biochemical, and molecular underpinnings of how these nutrients influence Cd bioavailability in the rhizosphere, Cd uptake and translocation pathways, sequestration and compartmentalization within plant tissues, and the activation of antioxidant defense systems. Nutrient elements exert their influence through diverse mechanisms: competing with Cd for root uptake transporters, promoting the synthesis of complexes that reduce Cd mobility, stabilizing cell walls and plasma membranes to restrict apoplastic flow and symplastic influx, modulating redox homeostasis by enhancing antioxidant enzyme activities and non-enzymatic antioxidant pools, regulating signal transduction pathways, and influencing gene expression profiles related to metal transport, chelation, and detoxification. The complex interactions between nutrients themselves further shape the plant’s capacity to withstand Cd stress. Recent advances elucidating nutrient-mediated epigenetic regulation, microRNA involvement, and the role of nutrient-sensing signaling hubs in Cd responses are critically evaluated. Furthermore, we synthesize the practical implications of nutrient management strategies, including optimized fertilization regimes, selection of nutrient-efficient genotypes, and utilization of nutrient-enriched amendments, for enhancing phytoremediation efficiency and developing low-Cd-accumulating crops, thereby contributing to safer food production and environmental restoration in Cd-contaminated soils. The intricate interplay between plant nutritional status and Cd stress resilience underscores the necessity for a holistic, nutrient-centric approach in managing Cd toxicity in agroecosystems. Full article

Toxic cyanobacteria, including Microcystis, produce harmful toxins that affect aquatic ecosystems and human health. Biotreatment using macrophytes shows promise in mitigating these blooms. This study investigates the bioaccumulation dynamics and biochemical responses of two aquatic macrophytes, Pistia stratiotes and Ceratophyllum demersum, in removing microcystin from contaminated water. P. stratiotes showed high initial bioaccumulation rates with rapid microcystin uptake, which is effective for short-term bioremediation. C. demersum has shown stable bioaccumulation. Biochemical analyses have revealed the activation of plant antioxidant defenses, with both macrophytes showing an increase in carotenoids, glutathione (GSH), and antioxidant enzymes such as superoxide dismutase (SOD) and glutathione-S-transferase (GST) concentrations. In particular, C. demersum has maintained higher antioxidant levels, contributing to its sustained capacity and resilience. Fluctuations in malondialdehyde (MDA) indicated oxidative stress, with P. stratiotes managing such stress through its defenses. Principal Component Analysis (PCA) supports these findings: Pistia’s first two components explained 25.09% and 20.71% of the variance, with Carotenoid and Chl contributing strongly to PC1, and MDA and GST influencing both components. For C. demersum, PC1 and PC2 explained 21.79% and 19.78% of the variance, with Carotenoid and Chl a being major contributors, while SOD and GSH played significant roles in sample differentiation. Integrating both plants into bioremediation strategies could optimize microcystin removal: P. stratiotes offers rapid initial detoxification, while C. demersum ensures continuous, long-term remediation. This combined approach enhances the efficiency and sustainability of phytoremediation. Future research should optimize environmental conditions and explore synergistic effects among multiple plant species for more effective and sustainable bioremediation solutions. Full article