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Keywords = uveal melanoma mass spectrometry imaging

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9 pages, 984 KB  
Communication
Multi-Modal Mass Spectrometric Imaging of Uveal Melanoma
by Laura M. Cole, Joshua Handley, Emmanuelle Claude, Catherine J. Duckett, Hardeep S. Mudhar, Karen Sisley and Malcolm R. Clench
Metabolites 2021, 11(8), 560; https://doi.org/10.3390/metabo11080560 - 23 Aug 2021
Cited by 3 | Viewed by 4072
Abstract
Matrix assisted laser desorption ionisation mass spectrometry imaging (MALDI-MSI), was used to obtain images of lipids and metabolite distribution in formalin fixed and embedded in paraffin (FFPE) whole eye sections containing primary uveal melanomas (UM). Using this technique, it was possible to obtain [...] Read more.
Matrix assisted laser desorption ionisation mass spectrometry imaging (MALDI-MSI), was used to obtain images of lipids and metabolite distribution in formalin fixed and embedded in paraffin (FFPE) whole eye sections containing primary uveal melanomas (UM). Using this technique, it was possible to obtain images of lysophosphatidylcholine (LPC) type lipid distribution that highlighted the tumour regions. Laser ablation inductively coupled plasma mass spectrometry images (LA-ICP-MS) performed on UM sections showed increases in copper within the tumour periphery and intratumoural zinc in tissue from patients with poor prognosis. These preliminary data indicate that multi-modal MSI has the potential to provide insights into the role of trace metals and cancer metastasis. Full article
(This article belongs to the Special Issue Spatial Metabolomics)
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