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Enzyme Biosensors: Challenges and Future Perspectives

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Special Issue Information

Dear Colleagues,

Recent advances in enzyme-based biosensors have propelled these devices to the forefront of analytical technologies, offering enhanced sensitivity, selectivity, and versatility in various applications. Enzyme-based biosensors leverage the catalytic activity of enzymes to convert biochemical reactions into measurable signals, providing valuable information about the presence and concentration of specific target analytes. However, the enzymes employed in these biosensors are intact and face challenges regarding stability, reproducibility, cost-effectiveness, biocompatibility, and biodegradation, underscoring the complexity and multi-faceted nature of biosensor development. Thus, researchers are actively seeking alternative approaches to address these challenges by exploring cutting-edge technologies, such as nanotechnology, advanced materials, microfluidics, and artificial intelligence, to promote the development of more robust, sensitive, and versatile analytical tools with broad applications in healthcare, environmental monitoring, and beyond.

Therefore, this Special Issue, “Enzyme Biosensors: Challenges and Future Perspectives”, encourages researchers and experts in the field to share their recent findings, insights, and methodologies to contribute to the collective knowledge base and drive further advancements in enzyme-based biosensors.

Dr. Pabudi Weerathunge
Prof. Dr. Guang-Bo Ge
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

enzymatic biosensors
artificial enzymes
enzyme-mimicking
nanozymes
nanosensors
point-of-care biosensors
electrochemical enzyme biosensors
optical enzyme biosensors
food safety biosensors
environmental biosensors

Published Papers (2 papers)

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Research

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11 pages, 3189 KiB

Open AccessArticle

Blood Coagulation-Inspired Fibrin Hydrogel for Portable Detection of Thrombin Based on Personal Glucometer

by Dan-Ni Yang, Shu-Yi Wu, Han-Yu Deng, Hao Zhang, Shan Shi and Shan Geng

Biosensors 2024, 14(5), 250; https://doi.org/10.3390/bios14050250 - 16 May 2024

Viewed by 570

Abstract

As one of the biomarkers of coagulation system-related diseases, the detection of thrombin is of practical importance. Thus, this study developed a portable biosensor based on a personal glucometer for rapid detection of thrombin activity. Fibrinogen was used for the detection of thrombin, and the assay principle was inspired by the blood coagulation process, where thrombin hydrolyzes fibrinogen to produce a fibrin hydrogel, and the amount of invertase encapsulated in the fibrin hydrogel fluctuates in accordance with the activity of thrombin in the sample solution. The quantitative assay is conducted by measuring the amount of unencapsulated invertase available to hydrolyze the substrate sucrose, and the signal readout is recorded using a personal glucometer. A linear detection range of 0–0.8 U/mL of thrombin with a limit of detection of 0.04 U/mL was obtained based on the personal glucometer sensing platform. The results of the selectivity and interference experiments showed that the developed personal glucometer sensing platform is highly selective and accurate for thrombin activity. Finally, the reliability of the portable glucometer method for rapid thrombin detection in serum samples was investigated by measuring the recovery rate, which ranged from 92.8% to 107.7%. In summary, the fibrin hydrogel sensing platform proposed in this study offers a portable and versatile means for detecting thrombin using a personal glucometer. This approach not only simplifies the detection process, but also eliminates the need for large instruments and skilled operators, and substantially reduces detection costs. Full article

(This article belongs to the Special Issue Enzyme Biosensors: Challenges and Future Perspectives)

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Review

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23 pages, 5990 KiB

Open AccessReview

Research Progress on the Application of Covalent Organic Framework Nanozymes in Analytical Chemistry

by Dongmei Yao, Ling Xia and Gongke Li

Biosensors 2024, 14(4), 163; https://doi.org/10.3390/bios14040163 - 29 Mar 2024

Viewed by 1051

Abstract

Covalent organic frameworks (COFs) are porous crystals that have high designability and great potential in designing, encapsulating, and immobilizing nanozymes. COF nanozymes have also attracted extensive attention in analyte sensing and detection because of their abundant active sites, high enzyme-carrying capacity, and significantly improved stability. In this paper, we classify COF nanozymes into three types and review their characteristics and advantages. Then, the synthesis methods of these COF nanozymes are introduced, and their performances are compared in a list. Finally, the applications of COF nanozymes in environmental analysis, food analysis, medicine analysis, disease diagnosis, and treatment are reviewed. Furthermore, we also discuss the application prospects of COF nanozymes and the challenges they face. Full article

(This article belongs to the Special Issue Enzyme Biosensors: Challenges and Future Perspectives)

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