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Role of Microorganisms and Their Metabolites in Agriculture, Food and the Environment 2.0

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Microbiology".

Deadline for manuscript submissions: 30 August 2024 | Viewed by 475

Special Issue Editor


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Guest Editor
1. Department of Microbiology, Prof. Wacław Dąbrowski Institute of Agricultural and Food Biotechnology–State Research Institute, 02-532 Warsaw, Poland
2. Institute of High Pressure Physics, Polish Academy of Sciences, 01-142 Warsaw, Poland
Interests: food microbiology; food safety; high pressure food processing; Alicyclobacillus acidoterrestris; bacteriophage; biopreservation; biocontrol; biobanking
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Special Issue Information

Dear Colleagues,

Even though people have been using microorganisms for thousands of years, they still surprise us with their possibilities.

The production of fermented foods is one of the oldest food processing technologies and has been known since ancient times. Nowadays, microorganisms are used in the production of foods not only as a strategy for food preservation but also for improving specific characteristics, such as flavor, aroma, texture and digestibility.

The antagonistic activity of microorganisms is widely used, both in biological plant protection to reduce environmental degradation due to the widespread use of fungicides and against food-borne pathogens to prevent disease.

In turn, the ability of microorganisms to adsorb, accumulate and degrade common and emerging pollutants has attracted the use of biological resources in treating contaminated food, feed and the environment.

This Special Issue will attempt to shed light on the molecular aspects and enhance the current research in the field of new high-throughput technologies, such as genomics, metagenomics, transcriptomics, metatranscriptomics and metabolomics, allowing a more thorough description of the microbial constituents of the different environments.

As volume 1 of the Special Issue “Role of Microorganisms and Their Metabolites in Agriculture, Food and the Environment” was successful, we reopen this issue again in the International Journal of Molecular Sciences (https://www.mdpi.com/journal/ijms, ISSN 1422-0067, IF 5.6, JCR Category Q1). This second Special Issue, “Role of Microorganisms and Their Metabolites in Agriculture, Food and the Environment 2.0”, welcomes original manuscripts and review articles addressing this hot topic.

Prof. Dr. Barbara Sokolowska
Guest Editor

Manuscript Submission Information

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Keywords

  • fermentation
  • biopreservation
  • biocontrol
  • biodegradation
  • bioremediation
  • bioaugmentation
  • omic-technologies

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Research

28 pages, 9935 KiB  
Article
Biological Characterization and Genomic Analysis of Three Novel Serratia- and Enterobacter-Specific Virulent Phages
by Dziyana Shymialevich, Stanisław Błażejak, Paulina Średnicka, Hanna Cieślak, Agnieszka Ostrowska, Barbara Sokołowska and Michał Wójcicki
Int. J. Mol. Sci. 2024, 25(11), 5944; https://doi.org/10.3390/ijms25115944 - 29 May 2024
Viewed by 211
Abstract
Due to the high microbiological contamination of raw food materials and the increase in the incidence of multidrug-resistant bacteria, new methods of ensuring microbiological food safety are being sought. One solution may be to use bacteriophages (so-called phages) as natural bacterial enemies. Therefore, [...] Read more.
Due to the high microbiological contamination of raw food materials and the increase in the incidence of multidrug-resistant bacteria, new methods of ensuring microbiological food safety are being sought. One solution may be to use bacteriophages (so-called phages) as natural bacterial enemies. Therefore, the aim of this study was the biological and genomic characterization of three newly isolated Serratia- and Enterobacter-specific virulent bacteriophages as potential candidates for food biocontrol. Serratia phage KKP_3708 (vB_Sli-IAFB_3708), Serratia phage KKP_3709 (vB_Sma-IAFB_3709), and Enterobacter phage KKP_3711 (vB_Ecl-IAFB_3711) were isolated from municipal sewage against Serratia liquefaciens strain KKP 3654, Serratia marcescens strain KKP 3687, and Enterobacter cloacae strain KKP 3684, respectively. The effect of phage addition at different multiplicity of infection (MOI) rates on the growth kinetics of the bacterial hosts was determined using a Bioscreen C Pro growth analyzer. The phages retained high activity in a wide temperature range (from −20 °C to 60 °C) and active acidity values (pH from 3 to 12). Based on transmission electron microscopy (TEM) imaging and whole-genome sequencing (WGS), the isolated bacteriophages belong to the tailed bacteriophages from the Caudoviricetes class. Genomic analysis revealed that the phages have linear double-stranded DNA of size 40,461 bp (Serratia phage KKP_3708), 67,890 bp (Serratia phage KKP_3709), and 113,711 bp (Enterobacter phage KKP_3711). No virulence, toxins, or antibiotic resistance genes were detected in the phage genomes. The lack of lysogenic markers indicates that all three bacteriophages may be potential candidates for food biocontrol. Full article
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