The Revolution in Breast Cancer Diagnostics: From Visual Inspection of Histopathology Slides to Using Desktop Tissue Analysers for Automated Nanomechanical Profiling of Tumours

Round 1

Reviewer 1 Report

This paper provides an excellent overview about the use of IT AFM in fast cancer biopsy. Some minor editorials are 

Line 33 :  1 in 8 women -> 1 of ? 

Line 110 :  Please write out the meaning of  "US"  

Line 144 - 149 : Suggestion to take it immediately after Line 133

Line 189 : write out the meaning of OA

Line 310 :  is 3,000 nm 3 µm ? Komma irritates

Line  349   Reconsider wording from " I have summarized......." to "as summarized below" 

Line 387 " I " and Line "388"  harmonize to "we"

Line 420  Sentence starting with "First.." is confusing _ please re-write 

Line 466 - 468 Please try to write these statements by marking them with bullets 

English is perfect

Author Response

please see attachment

Author Response File: Author Response.pdf

Reviewer 2 Report

The authors describe the development of portable desktop tissue analyzer based on indentation-type atomic force microscopy (IT-AFM) for fast nanomechanical profiling of tumors. The authors compare the current profiling of tumors such as H&E, MRI, PET, and X-Ray with IT-AFM to address its advantages. However, the authors do not provide figures that describe the principle of IT-AFM and profiling of tumors. Moreover, there are not a table summary the features that recent studies about IT-AFM.

Author Response

please see attachment

Author Response File: Author Response.pdf

Round 2

Reviewer 2 Report

The author should add a graph to describe the technology and biological mechanisms.

Author Response

Dear Reviewer,

> The author should add a graph to describe the technology and biological mechanisms.

I have included a new figure (Figure 2) and text to explain it (marked in red). I also needed to shift text to guarantee a logic flow.  

Thank you!

Best wishes,

Martin

Author Response File: Author Response.docx