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Applied Sciences
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Novel Approaches of Digital Holographic Microscopy in Cell Biology
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Novel Approaches of Digital Holographic Microscopy in Cell Biology

A special issue of Applied Sciences (ISSN 2076-3417). This special issue belongs to the section "Optics and Lasers".

Deadline for manuscript submissions: closed (20 March 2024) | Viewed by 1700

Special Issue Editors

Dr. Kersti Alm

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Guest Editor
Phase Holographic Imaging, 223 63 Lund, Sweden
Interests: tumor biology; imaging; digital holographic cytometry
Special Issues, Collections and Topics in MDPI journals
Prof. Dr. Anette Gjörloff Wingren

E-Mail Website
Guest Editor
Department of Biomedical Science, Faculty of Health and Society, Malmö University, 205 06 Malmö, Sweden
Interests: tumor biology; immunology; imaging; digital holographic cytometry; nanotechnology
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Cell culture as a method has developed into studying undisturbed cells in an environment as close as possible to the natural. Digital holographic microscopy is well suited for this task, as it causes no phototoxicity and the monitoring does not affect the cells. Even though the use of digital holographic microscopy in cell biology is well established by now, the development of new applications is frequent. This special issue is intended to collect and promote novel ways to apply digital holographic microscopy to study cells, and to show the wide spectrum of the technique and the possible applications. Our aim is to provide cell biology researchers with insight into a cell friendly, versatile method with high future potential within life science and medicine.

Dr. Kersti Alm
Prof. Dr. Anette Gjörloff Wingren
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Applied Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • digital holographic microscopy
  • cell culture
  • live cell imaging
  • cell morphology
  • non-invasive
  • cell monitoring

Published Papers (1 paper)

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Research

11 pages, 2369 KiB  
Communication
Application of Digital Holographic Imaging to Monitor Real-Time Cardiomyocyte Hypertrophy Dynamics in Response to Norepinephrine Stimulation
by Wahida Akter, Herman Huang, Jacquelyn Simmons and Alexander Y. Payumo
Appl. Sci. 2024, 14(9), 3819; https://doi.org/10.3390/app14093819 - 30 Apr 2024
Viewed by 477
Abstract
Cardiomyocyte hypertrophy, characterized by an increase in cell size, is associated with various cardiovascular diseases driven by factors including hypertension, myocardial infarction, and valve dysfunction. In vitro primary cardiomyocyte culture models have yielded numerous insights into the intrinsic and extrinsic mechanisms driving hypertrophic [...] Read more.
Cardiomyocyte hypertrophy, characterized by an increase in cell size, is associated with various cardiovascular diseases driven by factors including hypertension, myocardial infarction, and valve dysfunction. In vitro primary cardiomyocyte culture models have yielded numerous insights into the intrinsic and extrinsic mechanisms driving hypertrophic growth. However, due to limitations in current approaches, the dynamics of cardiomyocyte hypertrophic responses remain poorly characterized. In this study, we evaluate the application of the Holomonitor M4 digital holographic imaging microscope to track dynamic changes in cardiomyocyte surface area and volume in response to norepinephrine treatment, a model hypertrophic stimulus. The Holomonitor M4 permits non-invasive, label-free imaging of three-dimensional changes in cell morphology with minimal phototoxicity, thus enabling long-term imaging studies. Untreated and norepinephrine-stimulated primary neonatal rat cardiomyocytes were live-imaged on the Holomonitor M4, which was followed by image segmentation and single-cell tracking using the HOLOMONITOR App Suite software version 4.0.1.546. The 24 h treatment of cultured cardiomyocytes with norepinephrine increased cardiomyocyte spreading and optical volume as expected, validating the reliability of the approach. Single-cell tracking of both cardiomyocyte surface area and three-dimensional optical volume revealed dynamic increases in these parameters throughout the 24 h imaging period, demonstrating the potential of this technology to explore cardiomyocyte hypertrophic responses with greater temporal resolution; however, technological limitations were also observed and should be considered in the experimental design and interpretation of results. Overall, leveraging the unique advantages of the Holomonitor M4 digital holographic imaging system has the potential to empower future work towards understanding the molecular and cellular mechanisms underlying cardiomyocyte hypertrophy with enhanced temporal clarity. Full article
(This article belongs to the Special Issue Novel Approaches of Digital Holographic Microscopy in Cell Biology)
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